| Name |
Location |
Census Division |
Region |
Type |
Summary |
|---|
| Adelaide Hunter Hoodless Homestead | St. George | County of Brant | Golden Horseshoe | Historic house | website, mid-19th century childhood farm home of Adelaide Hunter Hoodless, founder of the Women's Institute |
| Agnes Etherington Art Centre | Kingston | Frontenac County | Eastern | Art | Operated by Queen's University, includes Canadian, European, Inuit and African art, decorative arts |
| Algonquin Provincial Park | Whitney | Nipissing District | Northeastern | Multiple | Includes the visitor center with exhibits about the area's natural and cultural history, and a logging museum |
| Alix Art Gallery | Sarnia | Lambton County | Southwestern | Art | website, includes paintings by the Group of Seven, contemporary and historically significant Canadian visual art and visual culture |
| Allan Macpherson House | Napanee | Lennox and Addington County | Eastern | Historic house | website, information, c.1830 period house, Georgian architecture built by Napanee's first postmaster and a prominent local figure, operated by the Lennox and Addington Historical Society |
| Ameliasburgh Historical Museum | Ameliasburgh | Prince Edward County | Central | Open air | information |
| Anderson Farm Museum | Lively | Greater Sudbury | Northeastern | Agriculture | Operated by Greater Sudbury Heritage Museums, historic 1920s period farmhouse, barn and milk house |
| Annandale National Historic Site | Tillsonburg | Oxford | Southwestern | Historic house | 1880s Victorian house known for its lavish Aesthetic art decorations |
| Arbor Gallery | Vankleek Hill | Prescott and Russell | Eastern | Art | website, contemporary art centre |
| Arkona Lions Museum | Arkona | Lambton County | Southwestern | Natural history | website, area Devonian fossils and First Nations artifacts |
| Arnprior & District Museum | Arnprior | Renfrew County | Eastern | Local history | website |
| Art Gallery of Algoma | Sault Ste. Marie | Algoma District | Northeastern | Art | Visual art |
| Art Gallery of Hamilton | Hamilton | Hamilton | Golden Horseshoe | Art | Includes Canadian and European works |
| Art Gallery of Mississauga | Mississauga | Peel Region | Golden Horseshoe | Art | Emphasis on area artists and Canadian art |
| Art Gallery of Northumberland | Cobourg | Northumberland | Central | Art | website |
| Art Gallery of Peterborough | Peterborough | Peterborough County | Central | Art | Focus is Canadian art |
| Art Gallery of Sudbury | Sudbury | Greater Sudbury | Northeastern | Art | Contemporary art |
| Art Gallery of Windsor | Windsor | Essex County | Southwestern | Art | Significant works of art by local, regional and national artists |
| Artcite | Windsor | Essex County | Southwestern | Art | website, artist-run contemporary and experimental art centre |
| Artery Gallery | Kitchener | Waterloo Region | Golden Horseshoe | Art | website, curated and managed by the student Society of Fine Artists of the University of Waterloo |
| Artspace Peterborough | Peterborough | Peterborough County | Central | Art | website, artist-run contemporary art centre |
| Arthur Child Heritage Museum | Gananoque | Leeds and Grenville | Eastern | Multiple | Area cultural and natural history, including 1000 Islands ecosystem, First Nations and early settlers, military and tourism exhibits, impact of the St. Lawrence River |
| Assiginack Museum Complex | Assiginack | Manitoulin District | Northeastern | Open air | website, information, includes the original late 19th-century town lock-up and jailer's home, pioneer home, school, blacksmith shop and driving shed, and the SS Norisle, a steam-powered automobile ferry museum ship |
| Atikokan Centennial Museum | Atikokan | Rainy River District | Northwestern | Local history | website, includes collection of logging, mining and railroad heavy equipment |
| Atrium Gallery | Ottawa | Ottawa | Eastern | Art | website, works of local and regional artists, owned and operated by the City of Ottawa |
| Aylmer & District Museum | Aylmer | Elgin County | Southwestern | Local history | website |
| Backus Mill Heritage and Conservation Centre | Port Rowan | Norfolk County | Southwestern | Open air | Includes 19th century period buildings, a working historic grist mill and a nature centre |
| Backus-Page House Museum | Wallacetown | Elgin County | Southwestern | Historic house | Operated by the Tyrconnell Heritage Society, mid-19th century period house |
| Bala's Museum | Bala | Muskoka | Central | Biographical | Life of author Lucy Maud Montgomery, author of Anne of Green Gables |
| Ball's Falls Centre for Conservation | Ball's Falls | Niagara Region | Golden Horseshoe | Natural history | Ecology of the Niagara Escarpment Biosphere Reserve, the natural history of the Twenty Valley and its watershed, and the area's cultural history |
| Bancroft Mineral Museum | Bancroft | Hastings County | Central | Natural history | information, information, area gems and minerals, operated by the Bancroft Gem and Mineral Club |
| Banting House National Historic Site | London | Middlesex County | Southwestern | Medical | Life of Sir Frederick Banting, doctor and co-discoverer of insulin |
| Barn Dance Historical Society Entertainment Museum | Wingham | Huron | Southwestern | Music | website, located inside the North Huron Museum, history of the barn dance radio show and its country music performers, information |
| Barnum House | Grafton | Northumberland | Central | Historic house | 19th century period house, operated by Ontario Heritage Trust |
| Base Borden Military Museum | Borden | Simcoe County | Central | Military | History of CFB Borden, includes planes, tanks, uniforms, weapons |
| Bath Museum | Bath | Lennox and Addington County | Eastern | Local history | website |
| Battle Ground Hotel Museum | Niagara Falls | Niagara Region | Golden Horseshoe | Historic house | website, 1850s period hotel on land that was part of the Battle of Lundy's Lane during the War of 1812 |
| Battle of the Windmill National Historic Site | Prescott | Leeds and Grenville | Eastern | Military | 60 foot stone lighthouse tower, site of the 1838 Battle of the Windmill |
| Battlefield House | Hamilton | Hamilton | Golden Horseshoe | Historic house | 1835 period house in park that was the site of the War of 1812 Battle of Stoney Creek |
| Beachville and District Museum | Beachville | Oxford County | Southwestern | Local history | website, includes Victorian period room displays, agriculture equipment and vehicles |
| Beaver River Museum | Beaverton | Durham Region | Central | History | website, operated by the Beaverton Thorah Eldon Historical Society, includes mid-19th century log cabin, 1900 period house and a jail |
| Bell Homestead National Historic Site | Brantford | County of Brant | Golden Horseshoe | Historic house | 1870s period home of Alexander Graham Bell, exhibits on his life, the invention of the telephone, and the origins of Canadian telephone operations to 1880 |
| Belleville Scout-Guide Museum | Belleville | Hastings County | Central | Scouting | website, open by appointment |
| Bellevue House National Historic Site | Kingston | Frontenac County | Eastern | Historic house | Late 1840s period home of Canada's first Prime Minister Sir John Alexander Macdonald |
| Benares Historic House | Mississauga | Peel Region | Golden Horseshoe | Historic house | website, early 20th century period house spanning four family generations |
| Bethune Memorial House National Historic Site | Gravenhurst | Muskoka | Central | Biographical | Life and achievements of Dr. Henry Norman Bethune |
| Bethune-Thompson House | Williamstown | Stormont, Dundas and Glengarry | Eastern | Historic house | website, late 18th-century house, operated by Ontario Heritage Trust |
| Billings Estate National Historic Site | Ottawa | Ottawa | Eastern | Historic house | 1828 home of early settler, costumed guides discuss city and region's history |
| Billy Bishop Home and Museum | Owen Sound | Grey County | Southwestern | Biographical | Late Victorian birthplace home of WW I flying ace Billy Bishop, also features artifacts from Canadians who fought in the air during World War One and World War Two |
| Blackwood Gallery | Mississauga | Peel Region | Golden Horseshoe | Art | website, contemporary art gallery, part of University of Toronto Mississauga |
| Blockhouse Museum | Merrickville | Leeds and Grenville | Eastern | Transportation | website, history of the Rideau Canal, photos, local history exhibits, located in a historic blockhouse, operated by the Merrickville and District Historical Society |
| Bobby Orr Hall of Fame | Parry Sound | Parry Sound District | Northeastern | Sports | Ice hockey history and memorabilia of Bobby Orr, located in the Charles W. Stockey Centre for the Performing Arts |
| Bonnechere Museum | Bonnechere | Renfrew County | Eastern | Multiple | website, local history, culture and natural history, including area Ordovician fossils, geology, First Nations artifacts, fur trade, timber industry |
| Bothwell-Zone Oil Museum | Bothwell | Chatham-Kent | Southwestern | Industry | website, oil industry powerhouse and equipment, operated by the Bothwell-Zone & District Historical Society |
| Bowmanville Museum | Bowmanville | Durham Region | Central | Historic house | website, 1900-1930 period home reflecting the lifestyle of a wealthy merchant family, operated by the Clarington Museums |
| Boyd Heritage Museum | Bobcaygeon | Kawartha Lakes | Central | Local history | website |
| Bradley Museum | Mississauga | Peel Region | Golden Horseshoe | Local history | website |
| Brant Museum and Archives | Brantford | County of Brant | Golden Horseshoe | Local history | website, operated by the Brant Historical Society, period room and business displays |
| Brantford and Area Sports Hall of Recognition | Brantford | County of Brant | Golden Horseshoe | Sports | website, photographs and memorabilia of area sports figures, including Wayne Gretzky |
| Brocksden Country School Museum | Stratford | Perth County | Southwestern | Education | information, 19th century schoolhouse, open by appointment |
| Brockville Museum | Brockville | Leeds and Grenville | Eastern | Local history | website |
| Brubacher House | Waterloo | Waterloo Region | Golden Horseshoe | Historic house | website, operated by the Conrad Grebel University College and the Mennonite Historical Society of Ontario, restored mid-19th century Pennsylvania German farmstead |
| Bruce County Museum and Cultural Centre | Southampton | Bruce County | Southwestern | Multiple | website, exhibits include local history, natural history, maritime, First Nations, agriculture, maritime, military and culture |
| Bruce Mines Museum | Bruce Mines | Algoma District | Northeastern | Local history | website |
| Bunker Military Museum | Cobalt | Timiskaming District | Northeastern | Military | website, information, includes artifacts from the Boer War to the present day |
| Burlington Art Centre | Burlington | Halton Region | Golden Horseshoe | Art | Contemporary art centre, focus on visual art and crafts |
| Buxton National Historic Site and Museum | North Buxton | Chatham-Kent | Southwestern | History | Historic buildings in Underground Railroad community |
| Bygone Days Heritage Village | Collingwood | Simcoe County | Central | Open air | website, mid 19th-century village buildings with authentic furnishings and artifacts |
| Bytown Museum | Ottawa | Ottawa | Eastern | History | City's early history and building of the Rideau Canal |
| Caledonia Grand Trunk Station | Caledonia | Haldimand County | Golden Horseshoe | Railway | Features local railway artifacts, displays and an operating “G” scale model train |
| Callander Bay Heritage Museum | Callander | Parry Sound District | Northeastern | Multiple | website, located in the home of Dr. Allan Roy Dafoe, who delivered and cared for the Dionne quintuplets; includes period doctor's office and dispensary displays, the Dionne quintuplets, a barber shop, period rooms, area logging and maritime history, and the Alex Dufresne Gallery of art |
| Cambridge Butterfly Conservatory | Cambridge | Waterloo Region | Golden Horseshoe | Natural history | website, butterfly conservatory with live butterflies, birds, plants and natural history exhibits |
| Cambridge Centre for the Arts | Cambridge | Waterloo Region | Golden Horseshoe | Art | website, includes an art gallery |
| Cambridge Galleries | Cambridge | Waterloo Region | Golden Horseshoe | Art | website, part of the Cambridge Public Library system with exhibition space at Queen's Square, Preston and Design at Riverside |
| Cambridge Sculpture Garden | Cambridge | Waterloo Region | Golden Horseshoe | Art | website, outdoor sculpture garden |
| Cameron Highlanders of Ottawa Museum | Ottawa | Ottawa | Eastern | Military | Regimental history and artefacts, located in Cartier Square Drill Hall |
| Campbellford Memorial Military Museum | Campbellford | Northumberland | Central | Military | information, information |
| Canada Agriculture Museum | Ottawa | Ottawa | Eastern | Agriculture | Demonstration farm with live animals, tractors exhibit |
| Canada Aviation Museum | Ottawa | Ottawa | Eastern | Aerospace | History of Canadian aviation, includes civilian and military aircraft |
| Canada Science and Technology Museum | Ottawa | Ottawa | Eastern | Science | Exhibits include Canadian inventors and inventions, communications, digital networks, space exploration, railroads, canoes |
| Canada South Science City | Windsor | Essex County | Southwestern | Science | Open to the public on weekends and summer |
| Canada's First Forestry Station | St. Williams | Norfolk County | Southwestern | Forestry | website, efforts to plant the area's forests |
| Canadian Automotive Museum | Oshawa | Durham Region | Central | Automobile | Features mostly Canadian-made cars, automotive history in Canada |
| Canadian Baseball Hall of Fame | St. Marys | Perth County | Southwestern | Sports | Great players, teams, and accomplishments of baseball in Canada |
| Canadian Bushplane Heritage Centre | Sault Ste. Marie | Algoma District | Northeastern | Aviation | History of bush flying and forest protection in Canada |
| Canadian Canoe Museum | Peterborough | Peterborough County | Central | Maritime | Canoe collection and how the canoe defines the Canadian character and spirit |
| Canadian Clay and Glass Gallery | Waterloo | Waterloo Region | Golden Horseshoe | Art | Historic and contemporary Canadian ceramic, glass and enamel art |
| Canadian Clock Museum | Deep River | Renfrew County | Eastern | Horology | website, heritage of Canada's many clock manufacturers and sellers from the early 19th century to the present |
| Canadian Drilling Rig Museum | Rainham Centre | Haldimand County | Golden Horseshoe | Industry | website, history of the early natural gas fields of Haldimand, Norfolk and Southern Ontario |
| Canadian Firefighters Museum | Port Hope | Northumberland | Central | Firefighting | website, includes vehicles, fire hydrants, working municipal fire alarm system, photo displays, helmets, turnout gear and tools of the trade |
| Canadian Football Hall of Fame | Hamilton | Hamilton | Golden Horseshoe | Sports | Great achievements in Canadian football |
| Canadian Forces Museum of Aerospace Defense | North Bay | Nipissing District | Northeastern | Military | website, evolution of air defence and the contributions Canadians have made, includes NORAD exhibit, located at CFB North Bay |
| Canadian Golf Hall of Fame | Oakville | Halton Region | Golden Horseshoe | Sports | Canada's golf heritage |
| Canadian Medical Hall of Fame | London | Middlesex County | Southwestern | Medical | Honors Canadians who have contributed to the understanding of disease and improving the health of people |
| Canadian Military Heritage Museum | Brantford | County of Brant | Golden Horseshoe | Military | website, includes weapons, uniforms, medals, models, artifacts |
| Canadian Museum of Hindu Civilization | Richmond Hill | York Region | Golden Horseshoe | Religious | website, essence of Hinduism in North America |
| Canadian Museum of Nature | Ottawa | Ottawa | Eastern | Natural history | Galleries include fossils and dinosaurs, Canadian mammals and birds, water, rocks and minerals |
| Canadian Soccer Hall of Fame | Vaughan | York Region | Golden Horseshoe | Sports | Achievements of top Canadian Footballers and Builders who have played and developed the game in Canadae |
| Canadian Transportation Museum & Heritage Village | Kingsville | Essex County | Southwestern | Multiple | website, includes village with late over 20 heritage buildings from the 18th century up to the late 1920s, and the museum with antique, vintage and hot rod cars and trucks |
| Canadian War Museum | Ottawa | Ottawa | Eastern | Military | Canada's military history, includes vehicles, weapons, uniforms, art, artillery, memorabilia |
| Canadian Warplane Heritage Museum | Hamilton | Hamilton | Golden Horseshoe | Aerospace | Military aircraft |
| Cannington Historical Museum | Cannington | Durham Region | Central | Open air | website, includes two log houses, a railway station and caboose, a meeting hall, a driving shed |
| Carleton Place and Beckwith Heritage Museum | Carleton Place | Lanark County | Eastern | Local history | website, local history, culture |
| Carleton University Art Gallery | Ottawa | Ottawa | Eastern | Art | website, part of Carleton University |
| Carman House Museum | Iroquois | Stormont, Dundas and Glengarry | Eastern | Living | information, information, 1835 period Loyalist house |
| Castle Kilbride | Baden | Waterloo Region | Golden Horseshoe | Historic house | Late 19th century Victorian house |
| Centennial Museum of Sheguiandah | Sheguiandah | Manitoulin District | Northeastern | Local history | website |
| Centennial Park | Thunder Bay | Thunder Bay District | Northwestern | Forestry | Features a museum and recreation of a 1910 logging camp, including train |
| Central Manatoulin Pioneer Museum | Mindemoya | Manitoulin District | Northeastern | History | information, information, operated by the Central Manitoulin Historical Society, includes a log home, blacksmith shop, barn and equipment, milk processing and butter making equipment, ice harvesting tools |
| Centrepointe Theatre Gallery | Ottawa | Ottawa | Eastern | Art | Works of local and regional artists, operated by the City of Ottawa |
| CFB Petawawa Military Museums | Petawawa | Renfrew County | Eastern | Military | website, complex includes the Canadian Airborne Forces Museum about the history of The Canadian Airborne Regiment, and the CFB Petawawa Military Museum with exhibits about the history of CFB Petawawa |
| Champlain Trail Museum and Pioneer Village | Pembroke | Renfrew County | Eastern | Open air | website, operated by the Ottawa Valley Historical Society |
| Chapleau Centennial Museum | Chapleau | Sudbury District | Northeastern | Local history | website |
| Chapple Museum | Chapple | Rainy River District | Northwestern | Local history | website |
| Chatham Railroad Museum | Chatham–Kent | Chatham-Kent | Southwestern | Railroad | website, early railroad equipment, located in a baggage car |
| Chatham-Kent Black Historical Society | Chatham-Kent | Chatham-Kent | Southwestern | African American | website, W.I.S.H. Centre features exhibits about area black history and historic sites |
| Chatham-Kent Museum | Chatham-Kent | Chatham-Kent | Southwestern | History | website, local history |
| Chesley Heritage & Woodworking Museum | Chesley | Bruce County | Southwestern | History | information, local history and area woodworking industry |
| Chesterville & District Heritage Centre | Chesterville | Stormont, Dundas and Glengarry | Eastern | History | information, local history |
| Chiefswood National Historic Site | Ohsweken | County of Brant | Golden Horseshoe | Historic house | website, mid-19th century birthplace home of Pauline Johnson, a First Nations author and performer |
| Christ Church Community Museum | Lakefield | Peterborough County | Central | History | website, local history |
| City of Ottawa Art Galleries | Ottawa | Ottawa | Eastern | Art | website, art galleries owned and operated by the City of Ottawa, includes ASP, Barbara Ann Scott, Centrepointe Theatre, City Hall, Gallery 112, Karsh-Masson, Studio and Trinity galleries |
| Clark House | Powassan | Parry Sound District | Northeastern | Historic house | information, operated by the Powassan and Area Historical Society |
| Clarke Museum | Kirby | Durham Region | Central | History | website, operated by the Clarington Museums, includes local history displays, one room schoolhouse, blacksmith shop, agricultural equipment |
| Cloyne & District Historical Society Pioneer Museum | Cloyne | Lennox and Addington County | Eastern | History | website, historic artifacts including tools, clothing, kitchen and other household effects |
| Cobalt Firefighters Museum | Cobalt | Timiskaming District | Northeastern | Firefighting | information |
| Cobalt Mining Museum | Cobalt | Timiskaming District | Northeastern | Mining | website, silver mining |
| Cochrane Railway and Pioneer Museum | Cochrane | Cochrane District | Northeastern | Railway | information, information, include railroad, local history and Tim Horton exhibits |
| Coldwater Canadiana Heritage Museum | Coldwater | Simcoe County | Central | Open air | website, includes 1830s log cabin homestead with historic furnishings and household items, replica general store and post office, display barn, blacksmith shop, print shop, carriage house, slaughter house |
| Collingwood Museum | Collingwood | Simcoe County | Central | History | website, local history including early pioneer life, local settlers, traditional logging practices and pioneer settlement |
| Comber & District Historical Society Museum | Lakeshore | Essex County | Southwestern | History | information, local history |
| Commanda General Store Museum | Commanda | Parry Sound District | Northeastern | History | information, 1885 High Victorian store with counters, flooring and goods from 1885–1934[1] |
| Copper Cliff Museum | Copper Cliff | Greater Sudbury | Northeastern | Historic house | Operated by Greater Sudbury Heritage Museums, turn-of-the-20th-century log cabin mining family home |
| Cornwall Community Museum | Cornwall | Stormont, Dundas and Glengarry | Eastern | History | information, local history |
Correctional Service of Canada Museum
Canada's Penitentiary Museum | Kingston | Frontenac County | Eastern | Prison | History of Kingston Penitentiary and other correctional centres (website), history of Canada's penitentiary system |
| Cottonwood Mansion | Selkirk | Haldimand County | Golden Horseshoe | Historic house | website, late 19th century Victorian mansion |
| Country Heritage Park | Milton | Halton Region | Golden Horseshoe | Agriculture | Formerly the Ontario Agricultural Museum, over 30 buildings depict agriculture and rural life over 170 years |
| Craigleith Heritage Depot | Blue Mountains | Grey County | Central | History | website, information, local history and information centre |
| Crawford Lake Conservation Area | Campbellville | Halton Region | Golden Horseshoe | First Nations | Features reconstructed 15th century Iroquoian village and interpretive programs |
| Criminals Hall of Fame | Niagara Falls | Niagara Region | Golden Horseshoe | Wax | Wax figures of notorious criminals, from mobsters to serial killers |
| Crull Rock Collection | Balmertown | Kenora District | Northwestern | Natural history | information, information, display of rocks and mineral specimens from around the world including local gold samples, located in the municipal office, part of the Red Lake Museum collection |
| Cumberland Heritage Village | Cumberland | Ottawa | Eastern | Living | website, 1920-1930s rural village |
| Currency Museum | Ottawa | Ottawa | Eastern | Numismatic | Operated by the Bank of Canada, features the National Currency Collection |
| Delhi Tobacco Museum and Heritage Centre | Delhi | Norfolk County | Southwestern | Local history | website, includes the agricultural and cultural development of the area |
| Diefenbunker Museum | Carp | Ottawa | Eastern | Military | Cold War bunker for the Canadian government |
| Dionne Quints Museum | North Bay | Nipissing District | Northeastern | Biographical | Birthplace and childhood home of the Dionne quintuplets |
| Discovery North Bay | North Bay | Nipissing District | Northeastern | History | website, formerly the North Bay Area Museum, local history |
| Discovery Harbour | Penetanguishene | Simcoe County | Central | Open air | website, recreated 19th century community with replica ships and smaller vessels, historic buildings, and an original officers' quarters |
| Discovery House Museum | Sarnia | Lambton County | Southwestern | History | website, local history |
| Donald Hughes Annex Museum | Ailsa Craig | Middlesex County | Southwestern | Local history | website, operated by the North Middlesex Historical Society |
| Doon Heritage Village | Kitchener | Waterloo Region | Golden Horseshoe | Living | 1914 rural life |
| Dorothy’s House Museum | Port Hope | Northumberland | Central | Historic house | website, operated by the East Durham Historical Society, 19th century Victorian worker's cottage, open seasonally |
| Dorset Heritage Museum | Dorset | Muskoka | Central | History | website, local history including early pioneer life, local settlers, traditional logging practices and pioneer settlement |
| Dryden & District Museum | Dryden | Kenora District | Northwestern | Local history | website |
| Dufferin County Museum and Archives | Rosemont | Simcoe County | Central | Multiple | website, local history exhibits, reconstructed 1918 pioneer home, art exhibits, collectibles |
| Duke Hunt Museum | Thunder Bay | Thunder Bay District | Northwestern | History | website, originally known as the Paipoonge Museum, located in Oliver Paipoonge, early settler household artifacts, community life |
| Dundas Museum | Dundas | Hamilton | Golden Horseshoe | History | website, local history |
| Dundurn Castle | Hamilton | Hamilton | Golden Horseshoe | Historic house | 40 room 19th century estate of Sir Allan Napier MacNab, one of Canada's first premiers; grounds include Hamilton Military Museum |
| Durham Art Gallery | Durham | Grey County | Southwestern | Art | website |
| Dynamic Earth | Sudbury | Greater Sudbury | Northeastern | Mining | Geology and mining for nickel and gold |
| Ear Falls Museum | Ear Falls | Kenora District | Northwestern | Local history | information, area's aboriginal peoples, gold mining and lumbering industries and transportation |
| Edinburgh Square Heritage and Cultural Centre | Cayuga | Haldimand County | Golden Horseshoe | Local history | Includes original 1857 jail cell and gypsum mining exhibits |
| Edison Museum (Ontario) | Vienna | Elgin County | Southwestern | Biographical | website, home on land of Thomas Edison's parents former home, features Edison authentic artifacts and furniture |
| Eldon House | London | Middlesex County | Southwestern | Historic house | 19th century period mansion and gardens |
| Elgin County Museum | St. Thomas | Elgin County | Southwestern | Local history | website |
| Elgin County Railway Museum | St. Thomas | Elgin County | Southwestern | Railway | Railroad engines, cars and equipment, located in 1913 former Michigan Central Railroad locomotive shops |
| Elgin Military Museum | St. Thomas | Elgin County | Southwestern | Military | Canadian military history of Elgin County and its residents |
| Elliot Lake Nuclear and Mining Museum | Elliot Lake | Algoma District | Northeastern | Mining | website, area's uranium mining history including the Canadian Mining Hall of Fame, also nuclear power, minerals, local history, logging, wildlife, marine artifacts |
| Elman W. Campbell Museum | Newmarket | York Region | Central | Local history | website |
| Englehart & Area Museum | Englehart | Timiskaming District | Northeastern | Local history | website, also art exhibits |
| Erland Lee Museum | Stoney Creek | Hamilton | Golden Horseshoe | Historic house | Late 19th century period Victorian home, recognized as the birthplace of the first Women's Institutes |
| Ermatinger-Clergue National Historic Site | Sault Ste. Marie | Algoma District | Northeastern | Historic house | website, 19th century period house and summer kitchen, and turn-of-the-20th-century blockhouse and gardens |
| Essex County Steam & Gas Engine Museum | McGregor | Essex County | Southwestern | Agriculture | website, antique engines, tractors and farm machinery |
| Eva Brook Donly Museum | Simcoe | Norfolk County | Southwestern | Historic house | Victorian period house, also gallery of local art and history |
| Fairfield Museum (Ontario) | Thamesville | Chatham-Kent | Southwestern | Local history | website |
| Fairfield-Gutzeit House | Bath | Lennox and Addington County | Eastern | Historic house | information, information, Victorian period house |
| Fanshawe Pioneer Village | London | Middlesex County | Southwestern | Living | 19th century village with over 30 original and replica buildings on 46 acres (190,000 m2) |
| Farmtown Park | Stirling | Hastings County | Central | Agriculture | website, previously known as Hastings County Museum of Agricultural Heritage, includes cheese factory, barn display, farm machinery, steam engines and tractors, and an indoor 1930s-1940s heritage village display with period stores |
| Fashion History Museum | Cambridge | Waterloo Region | Golden Horseshoe | Theme Collections | 10,000 historical garments and accessories dating from the mid 18th century to the present website |
| Fieldcote Memorial Park & Museum | Ancaster | Hamilton | Golden Horseshoe | Multiple | website, local history and art exhibits |
| First Hussars Museum | London | Middlesex County | Southwestern | Military | website, artifacts and memorabilia of the 1st Hussars, open by appointment |
| Flour Mill Museum | Sudbury | Greater Sudbury | Northeastern | Historic house | Operated by Greater Sudbury Heritage Museums, early 20th-century house and log cabin |
| Forest City Gallery | London | Middlesex County | Southwestern | Art | website, artist-run centre for contemporary practices in visual/media arts, performance, literature and music |
| Forest-Lambton Museum | Forest | Lambton County | Southwestern | Local history | website, 1890s period rooms, local history, natural history, military and doll displays |
| Fort Erie | Fort Erie | Niagara Region | Golden Horseshoe | Military | Restored War of 1812 period fort |
| Fort Erie Historical Museum | Ridgeway | Niagara Region | Golden Horseshoe | Local history | website, housed in the 1874 Bertie Township Hall |
| Fort Erie Railroad Museum | Ridgeway | Niagara Region | Golden Horseshoe | Railway | website, features a steam engine, historic railway station, area railroad history and artifacts |
| Fort Frances Museum | Fort Frances | Rainy River District | Northwestern | Local history | website, includes natural history, First Nations, fur traders, pioneers, forest industry, community development and growth, art exhibits |
| Fort George National Historic Site | Niagara-on-the-Lake | Niagara Region | Golden Horseshoe | Military | Restored War of 1812 period fort with living history demonstrations |
| Fort Henry National Historic Site | Kingston | Frontenac County | Eastern | Military | 19th-century British military fortress with costumed interpreters |
| Fort Malden National Historic Site | Amherstburg | Essex County | Southwestern | Military | Restored fort buildings and exhibits about the War of 1812, the Rebellion of 1837 and 19th century soldiers' lives |
| Fort St. Joseph National Historic Site | St. Joseph Island | Algoma District | Northeastern | Military | Ruins of War of 1812 fort, exhibits about the site's military history |
| Fort Wellington National Historic Site | Prescott | Leeds and Grenville | Eastern | Military | Restored 1846 period fort with living history demonstrators |
| Fort William Historical Park | Thunder Bay | Thunder Bay District | Northwestern | Living | Reconstructed 1815 fur trading post |
| Forwarders' Museum | Prescott | Leeds and Grenville | Eastern | Transportation | information, history of the forwarding of goods in the area before the construction of the Saint Lawrence Seaway |
| Founders' Museum & Pioneer Village | Thunder Bay | Thunder Bay District | Northwestern | Open air | website, pioneer village with buildings from different periods, located in Oliver Paipoonge |
| Foyer Gallery | Kanata | Ottawa | Eastern | Art | Artist-run gallery for regional art, located in the Nepean Sportsplex |
| Franco-Ontarian Folklore Centre | Sudbury | Greater Sudbury | Northeastern | Ethnic | website, trades and traditions of pioneers, antique musical instruments and the daily life of the first French-Canadian settlers information], heritage of the Franco-Ontarian settlers |
| Frederick Horsman Varley Art Gallery | Markham | York Region | Central | Art | Located in the historic house of artist Frederick Varley |
| Frontenac County Schools Museum | Kingston | Frontenac County | Eastern | Education | website, one-room rural school house in the 1900-1930 era |
| Fulford Place | Brockville | Leeds and Grenville | Eastern | Historic house | Edwardian era mansion, operated by Ontario Heritage Trust |
| Galerie du Nouvel-Ontario | Sudbury | Greater Sudbury | Northeastern | Art | Focus is contemporary art by Franco-Ontarian artists |
| Gallery 101 | Ottawa | Ottawa | Eastern | Art | website, artist-run contemporary art centre |
| Gallery of Early Canadian Flight | Wiarton | Bruce County | Southwestern | Aviation | website, features airplane models and photos, artist's prints, and posters of many of the historic moments in Canada's aviation history, located at Wiarton Airport |
| Gallery Stratford | Stratford | Perth County | Southwestern | Art | Exhibitions of local, national and international visual artists |
| Georgian College Campus Gallery | Barrie | Simcoe County | Central | Art | website, part of Georgian College in the Helen and Arch Brown Centre for Visual Art |
| Georgina Military Museum | Keswick | York Region | Golden Horseshoe | Military | website |
| Georgina Pioneer Village & Archives | Keswick | York Region | Golden Horseshoe | Open air | website, includes a log house, church, schoolhouse, general store, train station, blacksmith shop, apothecary |
| Gibson Gallery | Amherstburg | Essex County | Southwestern | Art | website, located in an 1896 former railroad station |
| Glanmore National Historic Site | Belleville | Hastings County | Central | Historic house | website, 1890s period mansion |
| Glengarry Pioneer Museum | Dunvegan | Stormont, Dundas and Glengarry | Eastern | Open air | Twelve 19th-century wooden buildings (including the oldest still-functioning bar in Ontario) in a rural setting, with historic tools and artifacts, farm equipment and machinery, furniture, household items, clothing and fabrics |
| Glengarry Sports Hall of Fame | Maxville | Stormont, Dundas and Glengarry | Eastern | Sports | website, achievements of Glengarry County's athletes |
| Glenhyrst Art Gallery of Brant | Brantford | County of Brant | Golden Horseshoe | Art | website, contemporary art |
| Gore Bay Museum | Gore Bay | Manitoulin District | Northeastern | Local history | information, information, also art exhibits, operated by the Western Manitoulin Historical Society |
| Goulbourn Museum | Stittsville | Ottawa | Eastern | Local history | website, including 19th and 20th century rural township life |
| Governor General's Foot Guards Regimental Museum | Ottawa | Ottawa | Eastern | Military | History of the regiment |
| Great War Flying Museum | Brampton | Peel Region | Golden Horseshoe | Aviation | Features flying replicas of World War I aircraft |
| Grey Roots Museum and Archives | Georgian Bluffs | Grey County | Southwestern | Local history | Open air heritage village in summer |
| Griffin House (Ancaster, Ontario) | Ancaster | Hamilton | Golden Horseshoe | Historic house | Owned and operated by the Hamilton Conservation Authority, 19th century period home, part of the Underground Railroad |
| Grimsby Museum | Grimsby | Niagara Region | Golden Horseshoe | Local history | website |
| Grimsby Public Art Gallery | Grimsby | Niagara Region | Golden Horseshoe | Art | website |
| Guelph Civic Museum | Guelph | Wellington County | Southwestern | Local history | Local and natural history |
| Guinness World Records | Niagara Falls | Niagara Region | Golden Horseshoe | Amusement | |
| Haileybury Heritage Museum | Haileybury | Timiskaming District | Northeastern | Local history | website, information, includes a streetcar, fire pumper, tugboat and caboose |
| Haldimand County Museum & Archives | Cayuga | Haldimand County | Golden Horseshoe | Local history | |
| Haliburton Highlands Museum | Haliburton | Haliburton County | Central | Local history | website, includes local history museum, log barn, pioneer house |
| Haliburton Sculpture Forest | Haliburton | Haliburton County | Central | Art | Outdoor sculpture park |
| Halton County Radial Railway | Milton | Halton Region | Golden Horseshoe | Transport | Includes electric streetcars, other railway vehicles, trolleybusses and buses |
| Halton Region Museum | Milton | Halton Region | Golden Horseshoe | Local history | |
| Hamilton Children's Museum | Hamilton | Hamilton | Golden Horseshoe | Children's | website |
| Hamilton Military Museum | Hamilton | Hamilton | Golden Horseshoe | Military | Canada's military history since the War of 1812; located on the ground of Dundurn Castle |
| Hamilton Museum of Steam & Technology | Hamilton | Hamilton | Golden Horseshoe | Technology | website, 19th century waterworks pumping station with two 45-foot (14 m) high, 70-ton steam engines |
| Hammond Museum of Radio | Guelph | Wellington County | Southwestern | Media | website, radios designed and manufactured in Canada, early radio and wireless artifacts, receivers and transmitters |
| Hastings and Prince Edward Regiment Museum | Belleville | Hastings County | Central | Military | Regimental uniforms, history and memorabilia |
| Heritage House Museum | Smiths Falls | Lanark County | Eastern | Historic house | information, Facebooks site, mid-19th century period Victorian home |
| Heritage Schoolhouse | Markham | York Region | Central | Education | website, 19th century schoolhouse museum |
| Hermitage and Gatehouse Museum | Ancaster | Hamilton | Golden Horseshoe | History | History of the ruins of the 19th-century house |
| Highlands Cinemeas and Movie Museum | Kinmount | Kawartha Lakes | Central | Media | website, small 5 screen movie theater and museum about movies, motion picture projectors and associated paraphernalia |
| Hillary House, The Koffler Museum of Medicine | Aurora | York Region | Golden Horseshoe | Medical | 19th-century house used as an office by three generations of doctors, features medical instruments, books, papers, household furnishings, and equipment dating from the early 19th to the late 20th century; operated by the Aurora Historical Society |
| Historic Cornwall Jail | Cornwall | Stormont, Dundas and Glengarry | Eastern | Prison | website, includes cell blocks, common areas, exercise yard and visitation area |
| HMCS Haida National Historic Site | Hamilton | Hamilton | Golden Horseshoe | Maritime | Tribal class destroyer museum ship |
| Hoch Farm | Killaloe, Hagarty and Richards | Renfrew County | Eastern | Historic house | information, 1910-20s period farmhouse |
| Hockey Heritage North | Kirkland Lake | Timiskaming District | Northeastern | Sports | Artifacts of local ice hockey history |
| Homer Watson House & Gallery | Kitchener | Waterloo Region | Golden Horseshoe | Art | website, gallery in home of artist Homer Watson |
| Homewood Museum (Maitland, Ontario) | Maitland | Leeds and Grenville | Eastern | Historic house | website, 1800 stone house, operated by Ontario Heritage Trust |
| Horseless Carriage Museum | Fenelon Falls | Kawartha Lakes | Central | Automobile | website, early automobiles and truck, engines, memorabilia |
| Humphrey Museum | Seguin | Parry Sound District | Northeastern | History | website, 1870s log schoolhouse with displays of pioneer history, also known as Seguin Township Museum |
| Huron County Marine Museum | Goderich | Huron | Southwestern | Maritime | website, lifestyle and culture of those who made a living on Lake Huron, open seasonally, included with admission to the Huron County Museum or Huron Historic Gaol |
| Huron County Museum | Goderich | Huron | Southwestern | Local history | website, furniture, agriculture, salt industry, area art and military displays |
| Huron Historic Gaol | Goderich | Huron | Southwestern | Prison | County jail from 1841 until 1972, courtrooms and early 20th century period governor's house |
| Huronia Museum | Midland | Simcoe County | Central | Multiple | Includes the 16th century period Huron Oendat Village, native art and archaeological collections, contemporary art gallery, local history and maritime exhibits, period room and general store displays |
| Hutchison House Museum | Peterborough | Peterborough County | Central | Living | website, operated by the Peterborough Historical Society, depicts 19th century life in Ontario |
| Hymers Museum | Kakabeka Falls | Thunder Bay District | Northwestern | History | information, local history and area's settlers, exhibits include mining, logging, farming, one-room schools, and the village's first church |
| Ignace Heritage Centre | Ignace | Kenora District | Northwestern | Local history | website, adjoins the Ignace Public Library |
| Ingersoll Cheese & Agricultural Museum | Ingersoll | Oxford | Southwestern | History | website, includes replica 20th-century cheese factory, a barn, local sports hall of fame, a working blacksmith shop and the Ingersoll Community Museum |
| Innisville Museum | Innisville | Lanark County | Eastern | Local history | information |
| International Hockey Hall of Fame | Kingston | Frontenac County | Eastern | Sports | History of ice hockey |
| Ireland House | Burlington | Halton Region | Golden Horseshoe | Historic house | website, homestead furnished for three periods: 1850s, 1890s and 1920s |
| Iron Bridge Museum | Iron Bridge | Algoma District | Northeastern | Local history | information |
| Iroquois Falls Pioneer Museum | Iroquois Falls | Cochrane District | Northeastern | Local history | website |
| Jack Seabrook's Farm Museum | Mindemoya | Manitoulin District | Northeastern | Agriculture | website, tractors, farm equipment and tools |
| Jet Aircraft Museum | London | Middlesex County | Southwestern | Aviation | Focus is Canadian Forces jet aircraft |
| John Freeman Walls Historic Site | Lakeshore | Essex County | Southwestern | Open air | 20-acre (81,000 m2) historical site that features historic buildings and was part of the Underground Railroad |
| John R. Park Homestead and Conservation Area | Harrow | Essex County | Southwestern | Historic house | website, 1850s house and farm buildings |
| Jolley's Riding Toy Museum | Meaford | Grey County | Southwestern | Toy | information, includes over 300 antique bicycles, tricycles, children’s wagons, scooters, pedal cars, hand cars, kiddie cars, ride-on vehicles, horses and sleds |
| Jordan Historical Museum of the Twenty | Jordan | Niagara Region | Golden Horseshoe | Historic house | Depicts early lives of the Loyalists and Pennsylvania Mennonites, includes 1835 period historic house with textiles and weaving displays, and a 1908 period schoolhouse |
| Joseph Brant Museum | Burlington | Halton Region | Golden Horseshoe | Local history | website, reconstructed early 19th century home of Joseph Brant features local history exhibits |
| Joseph Schneider Haus | Kitchener | Waterloo Region | Golden Horseshoe | Historic house | Early 19th century period homestead |
| Kanata Civic Art Gallery | Kanata | Ottawa | Eastern | Art | website, |
| Karsh-Masson Gallery | Ottawa | Ottawa | Eastern | Art | website, municipal art gallery |
| Kawartha Settlers' Village | Bobcaygeon | Kawartha Lakes | Central | Open air | website, historic homes and buildings from 1830–1915 |
| Kay-Nah-Chi-Wah-Nung | Stratton | Rainy River District | Northwestern | First Nations | Exhibits about the culture of the Ojibway people, tours of Manitou mounds |
| Keith M. Boyd Museum | Russell | Prescott and Russell | Eastern | Local history | website, operated by the Russell Historical Society |
| Killarney Museum | Killarney | Sudbury District | Northeastern | Local history | information |
| King Township Museum | King City | York Region | Golden Horseshoe | Local history | Located in a historic railroad station and church |
| Kingston Archaeological Centre | Kingston | Frontenac County | Eastern | Archaeology | website, history of the human occupation of Eastern Ontario over the past 8000 years |
| Kingston Scouting Museum | Kingston | Frontenac County | Eastern | Scouting | website, open by appointment |
| Kingsville Historical Park | Kingsville | Essex County | Southwestern | Military | website, includes the Charlie Campbell Museum of local military history and artifacts, and a restored 19th century range light |
| Kintail School | Lucknow | Bruce County | Southwestern | Education | information, late 19th-century schoolhouse |
| Kitchener-Waterloo Art Gallery | Kitchener | Waterloo Region | Golden Horseshoe | Art | website, emphasis is contemporary art |
| Komoka Railway Museum | Komoka | Middlesex County | Southwestern | Railway | website, housed in a 19th-century Canadian National Railway station, railroad artifacts and memorabilia |
| Lake of the Woods Museum | Kenora | Kenora District | Northwestern | Local history | website, themes include First Nations, fur traders, pioneer life, area industries and development |
| Lambton Heritage Museum | Lambton | Lambton County | Southwestern | Multiple | website, local history, pressed glass, agriculture equipment, antique vehicles and engines, late 19th century pioneer home, school, blacksmith shop |
| Lanark and District Museum | Lanark | Lanark County | Eastern | Local history | information |
| Lang Pioneer Village Museum | Peterborough | Peterborough County | Central | Living | 19th century pioneer village |
| Latcham Gallery | Stouffville | York Region | Central | Art | website |
| Laura Secord Homestead | Queenston | Niagara Region | Golden Horseshoe | Historic house | website, early 19th century period home of Laura Secord, a Canadian heroine of the War of 1812 |
| Laurier House National Historic Site | Ottawa | Ottawa | Eastern | Historic house | Home of two prime ministers |
| Lennox and Addington County Museum | Napanee | Lennox and Addington County | Eastern | Local history | Operated by the Lennox and Addington Historical Society |
| Library and Archives Canada | Ottawa | Ottawa | Eastern | Library | Art and history exhibits from its collections |
| Lincoln and Welland Regimental Museum | Niagara-on-the-Lake | Niagara Region | Golden Horseshoe | Military | History and artifacts of The Lincoln and Welland Regiment |
| Lindsay Gallery | Lindsay | Kawartha Lakes | Central | Art | website |
| Lithuanian Museum-Archives of Canada | Mississauga | Peel Region | Golden Horseshoe | Ethnic | website |
| Little Claybelt Homesteaders Museum | New Liskeard | Timiskaming District | Northeastern | Local history | website, local history and pioneer life in the area from the 1880s through the 1940s |
| Little Schoolhouse and Museum | South Baymouth | Manitoulin District | Northeastern | Local history | information, information, local history and one room school exhibits |
| Lockmaster's House Museum | Chaffey's Lock | Leeds and Grenville | Eastern | Transportation | website, local history exhibits, history of the Rideau Canal, located in a historic lockmaster's house |
| London Regional Children’s Museum | London | Middlesex County | Southwestern | Children's | |
| L'Orignal Jail | L'Orignal | Prescott and Russell | Eastern | Prison | Tours of the prison built in the 1820s and decommissioned in 1998 |
| Lorne Scots Regimental Museum | Brampton | Peel Region | Golden Horseshoe | Military | Regimental memorabilia |
| Lost Villages Museum | Long Sault | Stormont, Dundas and Glengarry | Eastern | Open air | Features buildings, photos and memorabilia of The Lost Villages; complex includes a general store, train station, log house, barber shop, school, church and home |
| Louis Tussaud's Waxworks | Niagara Falls | Niagara Region | Golden Horseshoe | Wax | website |
| Lucan Area Heritage & Donnelly Museum | Lucan | Middlesex County | Southwestern | Local history | website |
| Macaulay Heritage Park | Picton | Prince Edward County | Central | Historic house | website, includes 1850s period home, historic church with local history museum |
| Macdonald Stewart Art Centre | Guelph | Wellington County | Southwestern | Art | Exhibits of contemporary and historical art, craft and design |
| Mackenzie Printery & Newspaper Museum | Queenston | Niagara Region | Golden Horseshoe | Media | website, also website, reconstructed home of rebel publisher William Lyon Mackenzie, displays of 500 years of printing technology |
| MacLachlan Woodworking Museum | Kingston | Frontenac County | Eastern | Technology | website, 19th century life and technology, especially woodworking |
| MacLaren Art Centre | Barrie | Simcoe County | Central | Art | |
| Madonna House Apostolate | Combermere | Renfrew County | Eastern | History | Catholic Christian community, includes the Pioneer Museum with historic tools, household items and agriculture equipment |
| Mahoney Dolls House Gallery | Fort Erie | Niagara Region | Golden Horseshoe | Toy | website, features over 140 doll houses from 1780–1995 |
| Maidstone Bicentennial Museum | Lakeshore | Essex County | Southwestern | Local history | website |
| Maison Macdonell-Williamson House | Chute-à-Blondeau | Prescott and Russell | Eastern | Historic house | website, owned by Ontario Heritage Trust, operated by the Friends of the Macdonell-Williamson House, early 19th-century house |
| Marathon District Museum | Marathon | Thunder Bay District | Northwestern | Local history | information |
| Marie Dressler House | Cobourg | Northumberland | Central | Biographical | website, home of actress Marie Dressler, small museum |
| Marine Museum of the Great Lakes | Kingston | Frontenac County | Eastern | Maritime | Exhibits include shipbuilding, boat building, shipping, life of a sailor, marine art, natural history and ecology of the Great Lakes, and the museum ship CCGS Alexander Henry |
| Mariners Park Museum | Milford | Prince Edward County | Central | Open air | information |
| Markham Museum | Markham | York Region | Central | Open air | Buildings include houses, barns, sheds, train station, schoolhouse, general store, church, blacksmith, harness shop, saw mill, cider mill |
| Marten River Provincial Park | Marten River | Nipissing District | Northeastern | Forestry | Features replica 19th-century logging camp |
| Maryboro Lodge | Fenelon Falls | Kawartha Lakes | Central | Local history | website, also known as the Fenelon Museum |
| Massey Area Museum | Massey | Sudbury District | Northeastern | Local history | website, includes early 20th century general store display, First Nations artifacts, period rooms, lumbering, farming, mining and mineral displays |
| Mather-Walls House | Kenora | Kenora District | Northwestern | Historic house | website, information, turn-of-the-20th-century period house, owned by Ontario Heritage Trust, operated in the summer by the Lake of the Woods Historical Society |
| Mattawa Museum | Mattawa | Nipissing District | Northeastern | Local history | website, operated by the Mattawa and District Historical Society, information |
| McCrae House | Guelph | Wellington County | Southwestern | Historic house | Late 19th century period home of poet John McCrae |
| McDougall Cottage | Waterloo | Waterloo Region | Golden Horseshoe | Historic house | website, mid-19th-century Scottish labourer's home |
| McDougall Mill Museum | Renfrew | Renfrew County | Eastern | Local history | website, located in a former stone grist mill |
| McFarland House (Niagara-on-the-Lake, Ontario) | Niagara-on-the-Lake | Niagara Region | Golden Horseshoe | Historic house | website, early 19th century period house |
| McIntosh Gallery | London | Middlesex County | Southwestern | Art | website, part of the University of Western Ontario |
| McMaster Museum of Art | Hamilton | Hamilton | Golden Horseshoe | Art | Part of McMaster University, changing exhibits of historical, modern and contemporary art in five galleries |
| McMichael Canadian Art Collection | Kleinburg | York Region | Golden Horseshoe | Art | Features paintings by Tom Thomson, the Group of Seven and their contemporaries, and First Nations and Inuit artists |
| Meaford Museum | Meaford | Grey County | Southwestern | Local history | website |
| Memory Junction Railway Museum | Brighton | Northumberland | Central | Railway | Located in a former railway station, includes a locomotive, railroad cars, equipment, memorabilia |
| Middleville and District Museum | Middleville | Lanark County | Eastern | Local history | information |
| Military Communications and Electronics Museum | Kingston | Frontenac County | Eastern | Military | History of military communications |
| Miller Museum of Geology | Kingston | Frontenac County | Eastern | Natural history | website, part of Queen's University, rocks, minerals, fossils, area geology |
| Milner House | Chatham-Kent | Chatham-Kent | Southwestern | Historic house | website, turn-of-the-20th-century lifestyle of a successful industrialist and his family |
| Minden Hills Cultural Centre | Minden Hills | Haliburton County | Central | Multiple | website, includes the Agnes Jamieson Gallery with a collection of works by André Lapine; the Minden Hills Museum with historic buildings including a schoolhouse, church, pioneer log home, blacksmith shop, logging camp, agriculture and local history exhibits; and R.D. Lawrence Place with exhibits about the nature activities and writings of RD Lawrence |
| Mission House Museum & Gallery | Combermere | Renfrew County | Eastern | Multiple | website, local history and art |
| Mississagi Lighthouse | Little Current | Manitoulin District | Northeastern | Maritime | website |
| Mississippi Valley Textile Museum | Almonte | Lanark County | Eastern | Textile | website, history of the area's textile industry, modern fiber arts exhibits, textile processes and equipment |
| Modern Fuel | Kingston | Frontenac County | Eastern | Art | website, artist-run contemporary art centre |
| Moore Museum | Mooretown | Lambton County | Southwestern | Open air | website, includes local history exhibits, church, school, fire hall, log cabin, railroad station, Victoria cottage, agriculture hall, blacksmith shop |
| Morningstar Mill | St. Catharines | Niagara Region | Golden Horseshoe | Open air | website, includes historic working grist mill,turbine shed, blacksmith shop, millers house, icehouse, sawmill and barn |
| Movieland Wax Museum of the Stars (Ontario) | Niagara Falls | Niagara Region | Golden Horseshoe | Wax | website |
| MS Norgoma | Sault Ste. Marie | Algoma District | Northeastern | Maritime | Canadian package freighter and passenger ferry museum ship |
| Murney Tower | Kingston | Frontenac County | Eastern | Military | mid-19th century martello tower with period exhibits |
| Murray L. Moore Hydro Museum | Pembroke | Renfrew County | Eastern | Science | website, also known as the Pembroke Hydro Museum, operated by Ottawa River Power |
| Muséoparc Vanier Museopark | Ottawa | Ottawa | Eastern | Local history | website, local history of Vanier and the Francophone heritage of Ottawa |
| Museum in the Square | Brantford | County of Brant | Golden Horseshoe | Art | website, operated by the Brant Historical Society, collection of decorative and fine arts |
| Museum London | London | Middlesex County | Southwestern | Multiple | Art, local history |
| Museum of Classical Antiquities | Ottawa | Ottawa | Eastern | Archaeology | website, teaching collection of the University of Ottawa, pottery and artifacts of daily life from ancient Greek and Roman civilizations dating from the 7th century BC to the 7th century AD |
| Museum of Health Care | Kingston | Frontenac County | Eastern | Medical | Nursing and history of health care |
| Museum of Northern History | Kirkland Lake | Timiskaming District | Northeastern | Multiple | Located in the Sir Harry Oakes Chateau, owned by Ontario Heritage Trust, historic house with exhibits about Harry Oakes and area mining, local history, area wildlife, local art exhibits |
| Museum of Ontario Archaeology | London | Middlesex County | Southwestern | Archaeology | Part of the University of Western Ontario, Ontario's prehistoric past, adjacent reconstructed Neutral Nation village |
| Museum of Vision Science | Kitchener | Waterloo Region | Golden Horseshoe | Medical | Part of the University of Waterloo, history of vision science, housed in the School of Optometry |
| Museum on the Boyne | Alliston | Simcoe County | Central | Local history | website, includes 1851 log cabin and an 1858 English barn with agriculture equipment |
| Museum Strathroy-Caradoc | Strathroy-Caradoc | Middlesex County | Southwestern | Local history | website, located in 34 Frank which is also home to Strathroy Public Library and The Art Space gallery |
| Museum of Temporary Art | Victoria Road | City of Kawartha Lakes | Central | Art | website, housed in an restored Victorian general store, the MTA is a museum of the imagination including collage, painting, evolving installations and found art assemblages |
| Muskoka Boat & Heritage Centre | Gravenhurst | Muskoka | Central | Maritime | website, includes history of area steamships, wooden boats and luxury hotels, antique and wooden boat models, toys and Muskoka memorabilia |
| Muskoka Heritage Place | Huntsville | Muskoka | Central | Multiple | website, includes the Muskoka Museum, Muskoka Pioneer Village and the Huntsville & Lake of Bays Railway Society's Portage Flyer Train |
| Muskoka Lakes Museum | Port Carling | Muskoka | Central | Local history | |
| Myrtleville House Museum | Brantford | County of Brant | Golden Horseshoe | Historic house | website, operated by the Brant Historical Society, restored 1837 homestead with furnishings from 140 years of family life |
| Nancy Island Historic Site | Wasaga Beach | Simcoe County | Central | Military | website, information, includes artifacts from the 18th century schooner HMS Nancy, exhibits on the War of 1812 and a lighthouse |
| Naismith Museum | Almonte | Lanark County | Eastern | Biographical | Life of basketball inventor James Naismith |
| National Air Force Museum of Canada | Trenton | Hastings County | Central | Aviation | Formerly known as the RCAF Memorial Museum, Royal Canadian Air Force aircraft and artifacts |
| National Gallery of Canada | Ottawa | Ottawa | Eastern | Art | Collections include Canadian, European, Asian art from the Indian subcontinent, Tibet and China, contemporary art, photography, sculpture, indigenous art from around the world |
| Nepean Fine Arts League Gallery | Nepean | Ottawa | Eastern | Art | website |
| Nepean Museum | Nepean | Ottawa | Eastern | Local history | |
| Net Shed Museum | Meldrum Bay | Manitoulin District | Northeastern | Maritime | information, information, seafaring and fishing history of the region |
| Niagara Apothecary | Niagara-on-the-Lake | Niagara Region | Golden Horseshoe | Medical | website, owned by Ontario Heritage Trust, operated by the Ontario College of Pharmacists, restored 1869 pharmacy |
| Niagara Artists Centre | St. Catharines | Niagara Region | Golden Horseshoe | Art | website, artist-run contemporary art centre |
| Niagara Children's Museum | Niagara Falls | Niagara Region | Golden Horseshoe | Children's | website |
| Niagara Falls Art Gallery | Niagara Falls | Niagara Region | Golden Horseshoe | Art | website |
| Niagara Falls History Museum | Niagara Falls | Niagara Region | Golden Horseshoe | Local history | website, formerly the Lundy's Lane Historical Museum, features exhibits on the War of 1812 |
| Niagara Historical Museum | Niagara-on-the-Lake | Niagara Region | Golden Horseshoe | Local history | website |
| Niagara Pumphouse Visual Arts Centre | Niagara-on-the-Lake | Niagara Region | Golden Horseshoe | Art | website, features regional art |
| Niagara Scouting Museum | Niagara Falls | Niagara Region | Golden Horseshoe | Scouting | website, open by appointment |
| Nipissing Township Museum | Nipissing | Parry Sound District | Northeastern | Local history | website, website, includes pioneer artifacts, reconstructed homes, log church |
| No. 6 RCAF Dunnville Museum | Dunnville | Haldimand County | Golden Horseshoe | Aviation | website, history of the No.6 Service Flying Training School in WW II |
| Norfolk Arts Centre | Simcoe | Norfolk County | Southwestern | Art | website, another website, also known as Lynnwood Arts Centre, located in the 1851 Lynnwood House |
| North American Black Historical Museum | Amherstburg | Essex County | Southwestern | African American | Focus is the Underground Railroad, black history in Canada, includes 19th-century church and log cabin |
| North Hastings Heritage Museum | Bancroft | Hastings County | Central | Local history | information |
| North Huron Museum | Wingham | Huron | Southwestern | Local history | website |
| North Lanark Regional Museum | Appleton | Lanark County | Eastern | Local history | information, includes replicas of a country store and a pioneer log cabin |
| Northern Ontario Railroad Museum | Capreol | Greater Sudbury | Northeastern | Railway | Railroad cars, equipment, artifacts and memorabilia |
| Northwestern Ontario Sports Hall of Fame | Thunder Bay | Thunder Bay District | Northwestern | Sports | Area's sports heritage |
| Nor'Westers and Loyalist Museum | Williamstown | Stormont, Dundas and Glengarry | Eastern | History | website, story of Sir John Johnson and Loyalist followers who settled in town during the American Revolution in 1784, and the North West Company |
| Norwich and District Museum | Norwich | Oxford | Southwestern | Open air | website, includes 1889 Quaker Meeting House, a blacksmith shop, an 1830s saltbox farmhouse, a turn-of-the-20th-century Quaker schoolhouse, and two barns with agricultural and dairy farming displays and equipment |
| O'Hara Mill Homestead | Madoc | Hastings County | Central | Open air | website, includes 19th century saw mill, 1850 period house, carpenter shop, blacksmith shop, storage sheds, schoolhouse and log house |
| Oak Hall | Niagara Falls | Niagara Region | Golden Horseshoe | Historic house | View the mansion's restored Great Hall reception area, the dining room and the living room, and a collection of Niagara Falls art |
| Oakville Galleries | Oakville | Halton Region | Golden Horseshoe | Art | website |
| Oakville Museum | Oakville | Halton Region | Golden Horseshoe | Local history | website, historic estate and grounds with local history exhibits |
| Odette Sculpture Park | Windsor | Essex County | Southwestern | Art | Outdoor sculpture park |
| Oil Museum of Canada | Oil Springs | Lambton County | Southwestern | Industry | website, petroleum industry artifacts, site of the first commercial oil well in North America |
| Old Brittania Schoolhouse | Mississauga | Peel Region | Golden Horseshoe | Education | website, 19th century one room schoolhouse |
| Olde Gaol Museum | Lindsay | Kawartha Lakes | Central | History | website, information, local and Canadian history set in a jail building dating back to the 1860s, operated by the Victoria County Historical Society |
| Old Mill Heritage Centre and Post Office Museum | Kagawong | Manitoulin District | Northeastern | Multiple | information, information, includes the Kagawong Museum with exhibits on local history, agriculture, military, early sawmills, commercial shipping and fishing, a historic post office museum display, and an art gallery |
| Old Stone Mill | Delta | Leeds and Grenville | Eastern | Mill | website, 1810 stone grist mill and adjacent Museum of Industrial Technology with exhibits of early industries of the region including early agricultural equipment |
| Omàmiwininì Pimàdjwowin | Golden Lake | Renfrew County | Eastern | First Nations | website, also known as The Algonquin Way Cultural Centre, heritage of the Algonquins of Pikwàkanagàn First Nation |
| Ontario Provincial Police Museum | Orillia | Simcoe County | Central | Law enforcement | website, history of the Ontario Provincial Police |
| Ontario Regiment Museum | Oshawa | Durham Region | Central | Military | History of The Ontario Regiment (RCAC), includes uniforms, over 80 military vehicles, medals, arms, photographs |
| Orillia Museum of Art & History | Orillia | Simcoe County | Central | Multiple | website, art and local history |
| Orval Berry Museum | Frankford | Hastings County | Central | Local history | information |
| Oshawa Community Museum | Oshawa | Durham Region | Central | Historic house | Includes late 19th century Victorian Henry House, local history exhibits |
| Oshawa Military and Industrial Museum | Oshawa | Durham Region | Central | Military | Features large collection of military vehicles |
| Osgoode Township Historical Society and Museum | Osgood Township | Ottawa | Eastern | Local history | website |
| Ottawa Art Gallery | Ottawa | Ottawa | Eastern | Art | website, contemporary and 20th-century art |
| Ottawa School of Art Galleries | Ottawa | Ottawa | Eastern | Art | |
| Ottawa Sports Hall of Fame | Ottawa | Ottawa | Eastern | Sports | website, located in the Canadian Tire Centre |
| Owen Sound Marine & Rail Museum | Owen Sound | Grey County | Southwestern | Transport | website, housed in a former Canadian National Railway Station on the Owen Sound waterfront, focus is the area's maritime, rail and industrial past |
| Paddy Walker House | Kincardine | Bruce County | Southwestern | Local history | |
| Palmerston Railway Heritage Museum | Palmerston | Wellington County | Southwestern | Railroad | |
| Park House Museum | Amherstburg | Essex County | Southwestern | Historic house | 1850s period house and local history exhibits |
| Parks Canada Discovery Center, Hamilton | Hamilton | Hamilton | Golden Horseshoe | Multiple | website, history and natural history exhibits on Canada's national parks and historic sites |
| Parkwood Estate | Oshawa | Durham Region | Central | Historic house | Early 20th-century mansion and estate gardens |
| Paris Museum and Historical Society | Paris | County of Brant | Golden Horseshoe | Local history | website |
| Peel Art Gallery, Museum and Archives | Brampton | Peel Region | Golden Horseshoe | Multiple | Local history museum, art gallery |
| Pelee Island Heritage Centre | Pelee Island | Essex County | Southwestern | Multiple | website, local history and natural history |
| Penetanguishene Centennial Museum | Penetanguishene | Simcoe County | Central | Local history | website |
| Personal Computer Museum | Brantford | County of Brant | Golden Horseshoe | Technology | Personal computers and artifacts, open on select days |
| Perth Legion Hall of Remembrance | Perth | Lanark County | Eastern | Military | information |
| Perth Museum | Perth | Lanark County | Eastern | Local history | Includes four 19th century period rooms of the Roderick Matheson House |
| Petawawa Research Forest Visitor Centre | Petawawa | Renfrew County | Eastern | Forestry | website, forestry and natural history exhibits, demonstration forest |
| Petawawa Heritage Village | Petawawa | Renfrew County | Eastern | Living | website, mid-19th century rural Ontario village |
| Peterborough Lift Lock | Peterborough | Peterborough County | Central | Transportation | Construction and workings of the Trent Canal |
| Peterborough Museum & Archives | Peterborough | Peterborough County | Central | Art | Focus is Canadian art |
| Petroglyphs Provincial Park | Woodview | Peterborough County | Central | First Nations | History and spiritual significance of the park's petroglyphs |
| Petrolia Discovery | Petrolia | Lambton County | Southwestern | Industry | website, early days of the boom of the petroleum industry |
| Pickering Museum Village | Pickering | Durham Region | Central | Open air | website, 19th century village |
| Pinhey's Point Historic Site | Dunrobin | Ottawa | Eastern | Historic house | 19th century estate home and park |
| Polar Bear Habitat and Heritage Village | Cochrane | Cochrane District | Northeastern | Open air | website, includes the Hunta Museum with period business displays and antiques, the Cochrane Classic Riders Snowmobile Museum, a blacksmith shop, doctor's office, farm house, general store, service station, schoolhouse, farm implement dealer and more |
| Port Burwell Marine Museum and Historic Lighthouse | Port Burwell | Elgin County | Southwestern | Maritime | Lighthouse, area maritime and shipbuilding history |
| Port Colborne Historical & Marine Museum | Port Colborne | Niagara Region | Golden Horseshoe | Open air | website, includes historic houses, blacksmith shop, a lighthouse with maritime exhibits, historic vehicles, two mid-19th century log houses |
| Port Dover Harbour Museum | Port Dover | Norfolk County | Southwestern | Maritime | website, local fishing industry, shipping, sailing, shipwrecks and maritime heritage |
| Presqu'ile Provincial Park | Brighton | Northumberland | Central | Maritime | Includes the Lighthouse Interpretive Centre and a nature center |
| Prince Township Museum | Prince | Algoma District | Northeastern | Local history | website |
| Princess of Wales' Own Regiment Museum | Kingston | Frontenac County | Eastern | Military | website, history and artifacts of The Princess of Wales' Own Regiment, located in the armoury |
| Princeton District Museum | Princeton | Oxford County | Southwestern | Local history | website, local history and art exhibits |
| Proctor House Museum | Brighton | Northumberland | Central | Historic house | website |
| Pump House Steam Museum | Kingston | Frontenac County | Eastern | Technology | website, former water pumping station, exhibits on engines, railroads, steam launch boat, science |
| R. Tait McKenzie Memorial Museum | Mississippi Mills | Lanark County | Eastern | Art | Located in the Mill of Kintail Conservation Area, life and works by sculptor R. Tait McKenzie |
| Railcar Museum (Moosonee, Ontario) | Moosonee | Cochrane District | Northeastern | History | information |
| Rails End Gallery & Arts Centre | Haliburton | Haliburton County | Central | Art | website |
| Railway Museum of Eastern Ontario | Smiths Falls | Lanark County | Eastern | Railway | Historic railway station with railroad locomotives, cars, artifacts and memorabilia |
| Rainy River District Women's Institute Museum | Emo | Rainy River District | Northwestern | Local history | information, information, local history and pioneer life |
| Rainy River Railroad Heritage Museum | Rainy River | Rainy River District | Northwestern | Railway | information, exhibits displayed inside a restored railway passenger car, also features a caboose, restored steam engine and local history exhibits |
| Raisin River Heritage Centre | St. Andrews | Stormont, Dundas and Glengarry | Eastern | Local history | website, information, operated by the Cornwall Township Historical Society |
| Rayside-Balfour Museum | Chelmsford | Greater Sudbury | Northeastern | Local history | Operated by Greater Sudbury Heritage Museums, local history, agriculture, culture |
| Red Lake Regional Heritage Centre | Red Lake | Kenora District | Northwestern | Local history | website, themes include Aboriginal history, gold mining, fur trade, immigration, art exhibits |
| Reuben R. Sallows Gallery | Goderich | Huron | Southwestern | Art | website, changing exhibits of early 20th century photographs |
| Richmond Hill Heritage Centre | Richmond Hill | York Region | Central | Local history | Exhibits in an 1840s period cottage |
| Rideau Canal Visitor Centre | Smiths Falls | Lanark County | Eastern | Transportation | website, canal history and heritage |
| Rideau District Museum | Westport | Leeds and Grenville | Eastern | Local history | website |
| Rideau Hall | Ottawa | Ottawa | Eastern | Historic house | Tours of the official residence of the Governor General of Canada |
| Ridge House Museum | Ridgetown | Chatham-Kent | Southwestern | Historic house | website, 1875 period historic house |
| Ripley's Believe It or Not! | Niagara Falls | Niagara Region | Golden Horseshoe | Amusement | |
| RiverBrink Art Museum | Queenston | Niagara Region | Golden Horseshoe | Art | Historic house with 18th- and 19th-century Canadian paintings, sculpture and decorative art |
| Riverview Miniature Village | Peterborough | Peterborough County | Central | Commodity | website, display of miniature buildings of Peterborough and the Kawartha area |
| Robert Langen Art Gallery | Waterloo | Waterloo Region | Golden Horseshoe | Art | website, operated by the Wilfrid Laurier University, focus is contemporary Canadian art |
| Robert McLaughlin Gallery | Oshawa | Durham Region | Central | Art | Focus is modern and contemporary Canadian art |
| Rock Legends Wax Museum | Niagara Falls | Niagara Region | Golden Horseshoe | Wax | website |
| Rodman Hall Art Centre | St. Catharines | Niagara Region | Golden Horseshoe | Art | website, part of Brock University |
| Ron Morel Memorial Museum | Kapuskasing | Cochrane District | Northeastern | Railroad | information, information, includes a steam locomotive, two passenger cars and a caboose, railroad artifacts and model railroad display, also local history exhibits |
| Ronathahon:ni Cultural Centre | Akwesasne | Stormont, Dundas and Glengarry | Eastern | First Nations | website, formerly known as North American Traveling College, culture and history of the Mohawk Nation |
| Rose House Museum | Waupoos | Prince Edward County | Central | Historic house | information, early 19th-century house with furnishings from 5 generations |
| Ross Museum | Whitewater Region | Renfrew County | Eastern | History | website, includes period room and business displays, household items, farming tools, logging equipment |
| Rotunda Gallery | Kitchener | Waterloo Region | Golden Horseshoe | Art | website, municipal art gallery located in Kitchener City Hall |
| Royal Botanical Gardens | Burlington | Halton Region | Golden Horseshoe | Natural history | Features exhibits about plants, nature and science |
| Royal Canadian Legion Branch 72 Museum | Pembroke | Renfrew County | Eastern | Military | website, information, open by request and for special events, local branch of the Royal Canadian Legion |
| Royal Canadian Regiment Museum | London | Middlesex County | Southwestern | Military | Regimental history and artifacts |
| Royal Hamilton Light Infantry Heritage Museum | Hamilton | Hamilton | Golden Horseshoe | Military | Regimental history, open by appointment |
| Royal Military College of Canada Museum | Kingston | Frontenac County | Eastern | Military | Located in Fort Frederick, history of the college and the naval history of the site |
| Rydal Bank Community Hall and Church | Plummer Additional | Algoma District | Northeastern | Local history | |
| Ruthven Park National Historic Site | Cayuga | Haldimand County | Golden Horseshoe | Historic house | website, 19th-century mansion estate |
| Sainte-Marie among the Hurons | Midland | Simcoe County | Central | Living | Recreated 17th-century French Jesuit mission headquarters and Wendat Huron inhabitants |
| Sarah Jane Williams Heritage Centre | Bowmanville | Durham Region | Central | Local history | website, operated by the Clarington Museums, features exhibits about Dominion Organ and Piano Company, a large doll and toy collection, and changing history exhibits |
| Sault Ste. Marie Museum | Sault Ste. Marie | Algoma District | Northeastern | Local history | website |
| Sault Ste. Marie Canal National Historic Site | Sault Ste. Marie | Algoma District | Northeastern | Transport | Visitor center, guided tours of the canal locks |
| School House Museum | Deep River | Renfrew County | Eastern | Local history | website, history of Laurentian Hills |
| School On Wheels Railcar Museum | Clinton | Huron | Southwestern | Education | website, historic school car that traveled the region to bring education to children |
| Science North | Sudbury | Greater Sudbury | Northeastern | Science | |
| Scugog Shores Heritage Center and Archives | Port Perry | Durham Region | Central | Multiple | website, local First Nations history and local history, changing exhibits of local history, art, culture |
| Scugog Shores Museum | Port Perry | Durham Region | Central | Open air | website, mid-to-late 19th century village, Ojibway Heritage Interpretive Lands about the natural and cultural history of the Scugog Watershed prior to European settlement, |
| Secrets of Radar Museum | London | Middlesex County | Southwestern | Military | Story of the more than 6,000 Canadian World War II veterans who were recruited into a top-secret project during World War II involving radar |
| Serbian Centre of Windsor | Windsor | Essex County | Southwestern | Ethnic | website, display of Serbian culture and history |
| Sharon Temple National Historic Site | Sharon | York Region | Golden Horseshoe | Open air | Story of the Children of Peace and the early settlers of East Gwillimbury; features the Sharon Temple (1825-1832), the Doan House (1819), David Willson's Study (1829), an 1850s Log House and other buildings on 4.5 acres of park land; operated by the Sharon Temple Museum Society |
| Sheffield Park Black History & Cultural Museum | Clarksburg | Grey County | Southwestern | History | website, early Black pioneer families |
| Shenkman Arts Centre | Ottawa | Ottawa | Eastern | Art | website, includes the Trinity Art Gallery with works of local and regional artists, the Dust Evans Gallery of the Gloucester Pottery School, and the Ottawa School of Art Orléans Campus Gallery |
| Sibbald Point Provincial Park | Sutton | York Region | Central | Local history | Features the Sibbald Memorial Museum, a historic house and local history museum |
| Simcoe County Museum | Minesing | Simcoe County | Central | Local history | website, local history and culture, church, schoolhouse, railway station, farm equipment |
| Sioux Lookout Museum | Sioux Lookout | Kenora District | Northwestern | Local history | website, located on the second floor of the Sioux Lookout Heritage Railway Station |
| Sir John Johnson Manor House | Williamstown | Stormont, Dundas and Glengarry | Eastern | Historic house | information, late 18th century home of Sir John Johnson, 2nd Baronet |
| Ska-Nah-Doht Village and Museum | Mount Brydges | Middlesex County | Southwestern | First Nations | website, recreated longhouse village of 1,000 years ago and museum with archaeological artifacts |
| Sky Harbour Gallery | Goderich | Huron | Southwestern | Aviation | website, located at Goderich Airport, airport history in WW II, county aviaion history |
| Sombra Museum Cultural Centre | Sombra | Lambton County | Southwestern | Local history | website, includes Victorian period home and log cabin |
| South Grey Museum | Flesherton | Grey County | Southwestern | Local history | website |
| South of 60 Arts' Centre | Barry's Bay | Renfrew County | Eastern | Art | |
| Southwestern Ontario Heritage Village | Essex | Essex County | Southwestern | Open air | website, 19th-century and early 20th-century buildings including log cabins, a general store, barber/cobbler shop, church, school, and a transportation museum with antique and classic cars, wagons, trucks, motorcycles, and other memorabilia |
| Sovereign House | Bronte | Halton Region | Golden Horseshoe | Historic house | website, operated by the Bronte Historical Society, 19th century period house |
| Spencerville Mill | Spencerville | Leeds and Grenville | Eastern | Mill | website, stone grist mill |
| Spruce Lane Farmhouse | Oakville | Halton Region | Golden Horseshoe | Historic house | turn-of-the-20th-century farmhouse with costumed interpreters, located in Bronte Creek Provincial Park, which also includes a nature centre and children's farm |
| St. Catharines Museum | St. Catharines | Niagara Region | Golden Horseshoe | Multiple | website, city's history, history and impact of the Welland Canal, Ontario Lacrosse Hall of Fame and Museum, the Underground Railroad and African Canadian heritage |
| St. Edwards Township Museum | Tobermory | Bruce County | Southwestern | History | website, local and maritime history, also late 19th century log house open by request |
| St. Jacobs Mill | St. Jacobs | Waterloo Region | Golden Horseshoe | Multiple | website, includes the Maple Syrup Museum, St. Jacobs Quilt Gallery, electricity exhibit, Home Hardware exhibit, model train exhibit |
| St. Joseph Island Museum | St. Joseph Island | Algoma District | Northeastern | Open air | website, complex includes a log cabin, church, school, barn, store |
| St. Joseph Museum | Zurich | Huron | Southwestern | Local history | website, operated by the St. Joseph Historical Society |
| St. Marys Museum | St. Marys | Perth County | Southwestern | Local history | website |
| St. Thomas-Elgin Public Art Centre | St. Thomas | Elgin County | Southwestern | Art | website |
| Stanhope Heritage Discovery Museum | Stanhope | Haliburton County | Central | Local history | website, includes log chute |
| Stephen Leacock Museum | Orillia | Simcoe County | Central | Historic house | website, summer home of author and humorist Stephen Leacock, includes exhibits on his life and writings, and a gallery of Canadian writers and art |
| Stonechurch Museum of Art | Quinte West | Hastings County | Central | Art | website, works by Dennis Noble |
| Stones 'n Bones Museum | Sarnia | Lambton County | Southwestern | Natural history | website, fossils, minerals, gemstones, artifacts, shells, butterflies, insects, antlers, horns, dinosaurs |
| Stratford Perth Museum | Stratford | Perth County | Southwestern | Local history | website |
| Sturgeon River House Museum | Sturgeon Falls | Nipissing District | Northeastern | Local history | Includes fur trading, pioneers, blacksmith shop and ice house |
| Swords and Ploughshares Museum | Kars | Ottawa | Eastern | Military | Focus is the Citizen Soldier (the Militiaman and Reservist) at peace and at war, features many military trucks and vehicles |
| Sudbury Region Police Museum | Sudbury | Greater Sudbury | Northeastern | Law enforcement | information |
| Teeterville Pioneer Museum | Teeterville | Norfolk County | Southwestern | History | website, complex includes a log house, smoke house, wayside church, drive barn and early Windham and area artifacts |
| Thames Art Gallery | Chatham-Kent | Chatham-Kent | Southwestern | Art | website |
| Thames Valley Museum School | Norwich | Oxford | Southwestern | Education | website, early 20th century rural schoolhouse |
| Thamesville Old Town Hall Museum | Thamesville | Chatham-Kent | Southwestern | Local history | website |
| Thelma Miles Historical Museum | Matheson | Cochrane District | Northeastern | Local history | website |
| THEMUSEUM | Kitchener | Waterloo Region | Golden Horseshoe | Multiple | Formerly the Waterloo Regional Children's Museum, interactive exhibits for children, changing exhibits of culture, art, history, science |
| Thomas House (Oakville, Ontario) | Oakville | Halton Region | Golden Horseshoe | Historic house | website, 1830-1840s period pioneer home, operated by the Oakville Historical Society |
| Thorold & Beaverdams Historical Society Museum | Thorold | Niagara Region | Golden Horseshoe | Local history | website |
| Thunder Bay Art Gallery | Thunder Bay | Thunder Bay District | Northwestern | Art | Contemporary First Nations art |
| Thunder Bay Historical Museum | Thunder Bay | Thunder Bay District | Northwestern | Local history | |
| Thunder Bay Military Museum | Thunder Bay | Thunder Bay District | Northwestern | Military | website, area's military history, includes regimental memorabilia of The Lake Superior Scottish Regiment |
| Timber Village Museum | Blind River | Algoma District | Northeastern | Forestry | website, lumbering and logging tools, artifacts and equipment |
| Timmins Museum: National Exhibition Centre | Timmins | Cochrane District | Northeastern | Multiple | website, local history and art, recreated 1930's miner's home |
| Timmins Underground Gold Mine Tour | Timmins | Cochrane District | Northeastern | Mining | website, tour and artifacts |
| Tom Thomson Art Gallery | Owen Sound | Grey County | Southwestern | Art | website, features a large collection of memorabilia and works from Tom Thomson and the Group of Seven |
| Treasure Chest Museum | Paisley | Bruce County | Southwestern | History | website, antiques and collectibles, tools, household items, lamps, machinery, glassware, crockery, furniture |
| Tree Museum | Gravenhurst | Muskoka | Central | Art | website, outdoor sculpture gallery |
| Tweed and Area Heritage Centre | Tweed | Hastings County | Central | Local history | website |
| Uncle Tom's Cabin Historic Site | Dresden | Chatham-Kent | Southwestern | Open air | Complex includes home of Josiah Henson, former U.S. slave whose life story was the inspiration for the novel Uncle Tom's Cabin |
| Union Gallery | Kingston | Frontenac County | Eastern | Art | website, part of Queen's University, contemporary art |
| United Empire Loyalist Heritage Centre | Adolphustown | Lennox and Addington County | Eastern | History | website, history of the United Empire Loyalists settlers in the late 18th century |
| University of Waterloo Art Galleries | Waterloo | Waterloo Region | Golden Horseshoe | Art | website, part of the University of Waterloo |
| Upper Canada Village | Morrisburg | Stormont, Dundas and Glengarry | Eastern | Living | 1860s period village with over 40 buildings |
| Uxbridge Historical Centre | Uxbridge | Durham Region | Central | Local history | website |
| Vankleek Hill Museum | Vankleek Hill | Prescott and Russell | Eastern | Local history | website, operated by the Vankleek Hill & District Historical Society |
| Visual Arts Centre of Clarington | Bowmanville | Durham Region | Central | Art | website |
| Waba Cottage Museum and Gardens | White Lake | Renfrew County | Eastern | Historic house | website, also historic log church and log schoolhouse |
| Wallaceburg and District Museum | Wallaceburg | Chatham-Kent | Southwestern | Local history | |
| Waterford Heritage & Agricultural Museum | Waterford | Norfolk County | Southwestern | Local history | website, social history of Waterford and Old Townsend as well as Norfolk County agriculture |
| Waterloo County Gaol and Governor's House | Kitchener | Waterloo Region | Golden Horseshoe | Historic site | website, former jail and governor's house with historic and archaeological exhibits in the lobby |
| Waterloo Region Museum | Kitchener | Waterloo Region | Golden Horseshoe | Local history | Regional history and culture including First Nation's peoples, European settlement, manufacturing heyday, high tech sector, entrance to Doon Heritage Village |
| Watson's Mill | Manotick | Ottawa | Eastern | Mill | 19th century flour grist mill |
| Welland Historical Museum | Welland | Niagara Region | Golden Horseshoe | Local history | website, located in a former Carnie library |
| Wellands Canal Centre | St. Catharines | Niagara Region | Golden Horseshoe | Transportation | website, construction, engineering, operations and impact of the Welland Canal |
| Wellington County Museum and Archives | Fergus | Wellington County | Southwestern | Multiple | website, local history, decorative arts and art |
| Wellington Heritage Museum | Wellington | Prince Edward County | Central | Local history | information |
| West Parry Sound District Museum | Parry Sound | Parry Sound District | Northeastern | Local history | website, also known as Museum on Tower Hill, local history, themes include trapping, First Nations, shipping, logging, agriculture and cottaging, features a fire tower to climb |
| Westfield Heritage Village | Rockton | Hamilton | Golden Horseshoe | Open air | Over 30 historic buildings on a 3.4 square kilometres (840 acres) site |
| Whitchurch-Stouffville Museum | Vandorf | York Region | Central | History | website, complex includes an 1880s period farmhouse, 19th century schoolhouse, log cabin, and local history museum |
| White Water Gallery | North Bay | Nipissing District | Northeastern | Art | website, contemporary visual art |
| Whitehern Historic House and Garden | Hamilton | Hamilton | Golden Horseshoe | Historic house | mid-19th century urban estate with elements from the Georgian, Victorian and Edwardian periods |
| Wilberforce Red Cross Outpost & Historic House | Wilberforce | Haliburton County | Central | Medical | website, site of the first Red Cross Outpost Hospital in Ontario |
| Willoughby Historical Museum | Niagara Falls | Niagara Region | Golden Horseshoe | Local history | website, history of the community of Willoughby |
| Wilno Heritage Park | Wilno | Renfrew County | Eastern | Ethnic | website, include the Polish Kashub Heritage Museum & Skansen, Kashubian and Polish heritage |
| Wilson MacDonald Memorial School Museum | Selkirk | Haldimand County | Golden Horseshoe | Education | information, 1925 period one room schoolhouse, exhibits about poet Wilson MacDonald |
| Windsor's Community Museum | Windsor | Essex County | Southwestern | Local history | |
| Windsor Wood Carving Museum | Windsor | Essex County | Southwestern | Art | website, carved wood art |
| Woodchester Villa | Bracebridge | Muskoka | Central | Historic house | Late 19th century octagon house with local history exhibits |
| Woodland Cultural Centre | Brantford | County of Brant | Golden Horseshoe | First Nations | website, history, culture and art of the Anishinaabe and Onkwehon:we |
| Woodside National Historic Site | Kitchener | Waterloo Region | Golden Horseshoe | Historic house | 1890s Victorian childhood home of Prime Minister William Lyon Mackenzie King |
| Woodstock Art Gallery | Woodstock | Oxford | Southwestern | History | website, changing exhibits and permanent display of works by Florence Carlyle |
| Woodstock Museum | Woodstock | Oxford | Southwestern | Local history | website |
| Woodstock Peace Lighthouse | Woodstock | Oxford | Southwestern | Religious | website, Christian icon art, operated by the Studite Fathers |
| Workers Arts & Heritage Centre | Hamilton | Hamilton | Golden Horseshoe | Multiple | website, art, culture and heritage of working people in Canada |
| Workers' History Museum | Ottawa | Ottawa | Eastern | History | website, workers’ history and heritage, changes surrounding working culture, working people, and the workplace in all sectors of the economy in the National Capital Region and Ottawa Valley |
| Youngtown Rock N Roll Museum | Omemee | Kawartha Lakes | Central | Music | website, celebration of music from the '50s, '60s and '70s, with large collection of Neil Young and Beatles memorabilia |
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