MDFI

MyoD family inhibitor
Identifiers
Symbols	MDFI ; I-MF; I-mfa
External IDs	OMIM: 604971 HomoloGene: 21150 GeneCards: MDFI Gene
Gene ontology Molecular function • protein binding • transcription factor binding Cellular component • nucleus • cytoplasm Biological process • negative regulation of transcription from RNA polymerase II promoter • activation of JUN kinase activity • embryo development • dorsal/ventral axis specification • negative regulation of Wnt signaling pathway • cytoplasmic sequestering of transcription factor • negative regulation of DNA binding • embryonic skeletal system morphogenesis • trophoblast giant cell differentiation Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	4188	17240
Ensembl	ENSG00000112559	ENSMUSG00000032717
UniProt	Q99750	P70331
RefSeq (mRNA)	NM_001300804	NM_001109973
RefSeq (protein)	NP_001287733	NP_001103443
Location (UCSC)	Chr 6: 41.64 – 41.65 Mb	Chr 17: 47.82 – 47.83 Mb
PubMed search

MyoD family inhibitor is a protein that in humans is encoded by the MDFI gene.^[1][2][3]

Function

This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures.^[3]

Interactions

MDFI has been shown to interact with:

• C9orf86,^[4]
• GNAI2,^[4]
• MyoD,^[5]
• Myogenin,^[5] and
• SIX1.^[4]

References

1. Kraut N (Sep 1997). "The gene encoding I-mf (Mdfi) maps to human chromosome 6p21 and mouse chromosome 17". Mamm Genome 8 (8): 618–9. doi:10.1007/s003359900517. PMID 9250874.
2. Wang Q, Young TM, Mathews MB, Pe'ery T (Mar 2007). "Developmental regulators containing the I-mfa domain interact with T cyclins and Tat and modulate transcription". J Mol Biol 367 (3): 630–46. doi:10.1016/j.jmb.2007.01.020. PMC 1868487. PMID 17289077.
3. "Entrez Gene: MDFI MyoD family inhibitor".
4. Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
5. Chen CM, Kraut N, Groudine M, Weintraub H (Sep 1996). "I-mf, a novel myogenic repressor, interacts with members of the MyoD family". Cell 86 (5): 731–41. doi:10.1016/s0092-8674(00)80148-8. PMID 8797820.

Further reading

• Chen CM, Kraut N, Groudine M, Weintraub H (1996). "I-mf, a novel myogenic repressor, interacts with members of the MyoD family.". Cell 86 (5): 731–41. doi:10.1016/S0092-8674(00)80148-8. PMID 8797820.
• Kraut N, Snider L, Chen CM, Tapscott SJ, Groudine M (1999). "Requirement of the mouse I-mfa gene for placental development and skeletal patterning.". EMBO J. 17 (21): 6276–88. doi:10.1093/emboj/17.21.6276. PMC 1170953. PMID 9799236.
• Snider L, Thirlwell H, Miller JR, Moon RT, Groudine M, Tapscott SJ (2001). "Inhibition of Tcf3 binding by I-mfa domain proteins.". Mol. Cell. Biol. 21 (5): 1866–73. doi:10.1128/MCB.21.5.1866-1873.2001. PMC 86756. PMID 11238923.
• Ma R, Rundle D, Jacks J, Koch M, Downs T, Tsiokas L (2004). "Inhibitor of myogenic family, a novel suppressor of store-operated currents through an interaction with TRPC1.". J. Biol. Chem. 278 (52): 52763–72. doi:10.1074/jbc.M309610200. PMID 14530267.
• Mizugishi K, Hatayama M, Tohmonda T, Ogawa M, Inoue T, Mikoshiba K, Aruga J (2004). "Myogenic repressor I-mfa interferes with the function of Zic family proteins.". Biochem. Biophys. Res. Commun. 320 (1): 233–40. doi:10.1016/j.bbrc.2004.05.158. PMID 15207726.
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (2005). "Towards a proteome-scale map of the human protein-protein interaction network.". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
• Lim J, Hao T, Shaw C, Patel AJ, Szabó G, Rual JF, Fisk CJ, Li N, Smolyar A, Hill DE, Barabási AL, Vidal M, Zoghbi HY (2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration.". Cell 125 (4): 801–14. doi:10.1016/j.cell.2006.03.032. PMID 16713569.