MMP8

Matrix metallopeptidase 8
PDB rendering based on 1a85.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1A85​, 1A86​, 1BZS​, 1I73​, 1I76​, 1JAN​, 1JAO​, 1JAP​, 1JAQ​, 1JH1​, 1JJ9​, 1KBC​, 1MMB​, 1MNC​, 1ZP5​, 1ZS0​, 1ZVX​, 2OY2​, 2OY4​, 3DNG​, 3DPE​, 3DPF​, 3TT4​
Identifiers
Symbols	MMP8 ; CLG1; HNC; MMP-8; PMNL-CL
External IDs	OMIM: 120355 MGI: 1202395 HomoloGene: 22482 ChEMBL: 4588 GeneCards: MMP8 Gene
EC number	3.4.24.34
Gene ontology Molecular function • metalloendopeptidase activity • serine-type endopeptidase activity • calcium ion binding • zinc ion binding Cellular component • extracellular region • proteinaceous extracellular matrix • extracellular space Biological process • proteolysis • extracellular matrix disassembly • extracellular matrix organization • collagen catabolic process • endodermal cell differentiation Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	4317	17394
Ensembl	ENSG00000118113	ENSMUSG00000005800
UniProt	P22894	O70138
RefSeq (mRNA)	NM_001304441	NM_008611
RefSeq (protein)	NP_001291370	NP_032637
Location (UCSC)	Chr 11: 102.71 – 102.73 Mb	Chr 9: 7.56 – 7.57 Mb
PubMed search

Neutrophil collagenase, also known as matrix metalloproteinase-8 (MMP-8) or PMNL collagenase (MNL-CL), is a collagen cleaving enzyme which is present in the connective tissue of most mammals.^[1] In humans, the MMP-8 protein is encoded by the MMP8 gene.^[2][3]

Function

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is stored in secondary granules within neutrophils and is activated by autolytic cleavage. Its function is degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.^[1]

References

1. "Entrez Gene: MMP8 matrix metallopeptidase 8 (neutrophil collagenase)".
2. Hasty KA, Pourmotabbed TF, Goldberg GI, Thompson JP, Spinella DG, Stevens RM, Mainardi CL (July 1990). "Human neutrophil collagenase. A distinct gene product with homology to other matrix metalloproteinases". J. Biol. Chem. 265 (20): 11421–4. PMID 2164002.
3. Devarajan P, Mookhtiar K, Van Wart H, Berliner N (June 1991). "Structure and expression of the cDNA encoding human neutrophil collagenase". Blood 77 (12): 2731–8. PMID 1646048.

Further reading

• Chandler S, Miller KM, Clements JM, et al. (1997). "Matrix metalloproteinases, tumor necrosis factor and multiple sclerosis: an overview.". J. Neuroimmunol. 72 (2): 155–61. doi:10.1016/S0165-5728(96)00179-8. PMID 9042108.
• Massova I, Kotra LP, Fridman R, Mobashery S (1998). "Matrix metalloproteinases: structures, evolution, and diversification.". FASEB J. 12 (12): 1075–95. doi:10.1142/S0217984998001256. PMID 9737711.
• Nagase H, Woessner JF (1999). "Matrix metalloproteinases.". J. Biol. Chem. 274 (31): 21491–4. doi:10.1074/jbc.274.31.21491. PMID 10419448.
• Bläser J, Triebel S, Reinke H, Tschesche H (1992). "Formation of a covalent Hg-Cys-bond during mercurial activation of PMNL procollagenase gives evidence of a cysteine-switch mechanism.". FEBS Lett. 313 (1): 59–61. doi:10.1016/0014-5793(92)81184-N. PMID 1330697.
• Devarajan P, Mookhtiar K, Van Wart H, Berliner N (1991). "Structure and expression of the cDNA encoding human neutrophil collagenase.". Blood 77 (12): 2731–8. PMID 1646048.
• Bläser J, Knäuper V, Osthues A, et al. (1992). "Mercurial activation of human polymorphonuclear leucocyte procollagenase.". Eur. J. Biochem. 202 (3): 1223–30. doi:10.1111/j.1432-1033.1991.tb16494.x. PMID 1662606.
• Knäuper V, Krämer S, Reinke H, Tschesche H (1990). "Characterization and activation of procollagenase from human polymorphonuclear leucocytes. N-terminal sequence determination of the proenzyme and various proteolytically activated forms.". Eur. J. Biochem. 189 (2): 295–300. doi:10.1111/j.1432-1033.1990.tb15489.x. PMID 2159879.
• Hasty KA, Pourmotabbed TF, Goldberg GI, et al. (1990). "Human neutrophil collagenase. A distinct gene product with homology to other matrix metalloproteinases.". J. Biol. Chem. 265 (20): 11421–4. PMID 2164002.
• Knäuper V, Krämer S, Reinke H, Tschesche H (1990). "Partial amino acid sequence of human PMN leukocyte procollagenase.". Biol. Chem. Hoppe-Seyler. 371 Suppl: 295–304. PMID 2169256.
• Knäuper V, Krämer S, Reinke H, Tschesche H (1990). "Partial amino-acid sequence of human PMN leukocyte procollagenase.". Biol. Chem. Hoppe-Seyler 371 (8): 733. doi:10.1515/bchm3.1990.371.2.733. PMID 2169766.
• Mallya SK, Mookhtiar KA, Gao Y, et al. (1991). "Characterization of 58-kilodalton human neutrophil collagenase: comparison with human fibroblast collagenase.". Biochemistry 29 (47): 10628–34. doi:10.1021/bi00499a008. PMID 2176876.
• Stams T, Spurlino JC, Smith DL, et al. (1995). "Structure of human neutrophil collagenase reveals large S1' specificity pocket.". Nat. Struct. Biol. 1 (2): 119–23. doi:10.1038/nsb0294-119. PMID 7656015.
• Fosang AJ, Last K, Neame PJ, et al. (1995). "Neutrophil collagenase (MMP-8) cleaves at the aggrecanase site E373-A374 in the interglobular domain of cartilage aggrecan.". Biochem. J. 304 (2): 347–51. PMC 1137499. PMID 7998967.
• Bode W, Reinemer P, Huber R, et al. (1994). "The X-ray crystal structure of the catalytic domain of human neutrophil collagenase inhibited by a substrate analogue reveals the essentials for catalysis and specificity.". EMBO J. 13 (6): 1263–9. PMC 394940. PMID 8137810.
• Fosang AJ, Last K, Knäuper V, et al. (1993). "Fibroblast and neutrophil collagenases cleave at two sites in the cartilage aggrecan interglobular domain.". Biochem. J. 295 (1): 273–6. PMC 1134849. PMID 8216228.
• Reinemer P, Grams F, Huber R, et al. (1994). "Structural implications for the role of the N terminus in the 'superactivation' of collagenases. A crystallographic study.". FEBS Lett. 338 (2): 227–33. doi:10.1016/0014-5793(94)80370-6. PMID 8307185.
• Thomas DB, Davies M, Peters JR, Williams JD (1993). "Tamm Horsfall protein binds to a single class of carbohydrate specific receptors on human neutrophils.". Kidney Int. 44 (2): 423–9. doi:10.1038/ki.1993.260. PMID 8397318.
• Cole AA, Chubinskaya S, Schumacher B, et al. (1996). "Chondrocyte matrix metalloproteinase-8. Human articular chondrocytes express neutrophil collagenase.". J. Biol. Chem. 271 (18): 11023–6. doi:10.1074/jbc.271.18.11023. PMID 8631924.
• Nakahara Y, Miyata T, Hamuro T, et al. (1996). "Amino acid sequence and carbohydrate structure of a recombinant human tissue factor pathway inhibitor expressed in Chinese hamster ovary cells: one N-and two O-linked carbohydrate chains are located between Kunitz domains 2 and 3 and one N-linked carbohydrate chain is in Kunitz domain 2.". Biochemistry 35 (20): 6450–9. doi:10.1021/bi9524880. PMID 8639592.
• Pendás AM, Santamaría I, Alvarez MV, et al. (1997). "Fine physical mapping of the human matrix metalloproteinase genes clustered on chromosome 11q22.3.". Genomics 37 (2): 266–8. doi:10.1006/geno.1996.0557. PMID 8921407.

External links

• The MEROPS online database for peptidases and their inhibitors: M10.002