Methyl halide transferase

Methyl halide transferase
Identifiers
EC number	2.1.1.165
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Methyl halide transferase (EC 2.1.1.165, MCT, methyl chloride transferase, S-adenosyl-L-methionine:halide/bisulfide methyltransferase, AtHOL1, AtHOL2, AtHOL3, HMT, S-adenosyl-L-methionine: halide ion methyltransferase, SAM:halide ion methyltransferase) is an enzyme with systematic name S-adenosylmethionine:iodide methyltransferase.^{[1][2][3][4][5][6]} This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + iodide $\rightleftharpoons$ S-adenosyl-L-homocysteine + methyl iodide

This enzyme contributes to the methyl halide emissions from Arabidopsis thaliana.

Chlorine too

The salt marsh plant Batis maritima contains the enzyme methyl chloride transferase that catalyzes the synthesis of chloromethane (CH₃Cl) from S-adenosine-L-methionine and chloride.^[7] This protein has been purified and expressed in E. coli, and seems to be present in other organisms such as white rot fungi (Phellinus pomaceus),^[2] red algae (Endocladia muricata), and the ice plant (Mesembryanthemum crystallinum), each of which is a known CH₃Cl producer.^[7][8]

References

1. Ni, X. and Hager, L.P. (1999). "Expression of Batis maritima methyl chloride transferase in Escherichia coli". Proc. Natl. Acad. Sci. USA 96 (7): 3611–3615. doi:10.1073/pnas.96.7.3611. PMC 22342. PMID 10097085.
2. Saxena, D., Aouad, S., Attieh, J. and Saini, H.S. (1998). "Biochemical characterization of chloromethane emission from the wood-rotting fungus Phellinus pomaceus". Appl. Environ. Microbiol. 64 (8): 2831–2835. PMC 106779. PMID 9687437.
3. Attieh, J.M., Hanson, A.D. and Saini, H.S. (1995). "Purification and characterization of a novel methyltransferase responsible for biosynthesis of halomethanes and methanethiol in Brassica oleracea". J. Biol. Chem. 270 (16): 9250–9257. doi:10.1074/jbc.270.16.9250. PMID 7721844.
4. Itoh, N., Toda, H., Matsuda, M., Negishi, T., Taniguchi, T. and Ohsawa, N. (2009). "Involvement of S-adenosylmethionine-dependent halide/thiol methyltransferase (HTMT) in methyl halide emissions from agricultural plants: isolation and characterization of an HTMT-coding gene from Raphanus sativus (daikon radish)". BMC Plant Biol. 9: 116–116. doi:10.1186/1471-2229-9-116. PMC 2752461. PMID 19723322.
5. Ohsawa, N., Tsujita, M., Morikawa, S. and Itoh, N. (2001). "Purification and characterization of a monohalomethane-producing enzyme S-adenosyl-L-methionine: halide ion methyltransferase from a marine microalga, Pavlova pinguis". Biosci. Biotechnol. Biochem. 65 (11): 2397–2404. doi:10.1271/bbb.65.2397. PMID 11791711.
6. Nagatoshi, Y.and Nakamura, T. (2007). "Characterization of three halide methyltransferases in Arabidopsis thaliana". Plant Biotechnol. 24: 503–506. doi:10.5511/plantbiotechnology.24.503.
7. Ni X, Hager LP (1998). "cDNA Cloning of Batis maritima Methyl Chloride Transferase and Purification of the Enzyme". Proc Natl Acad Sci USA 95 (22): 12866–71. doi:10.1073/pnas.95.22.12866. PMC 23635. PMID 9789006.
8. Ni X, Hager LP (1999). "Expression of Batis maritima Methyl Chloride Transferase in Escherichia coli". Proc Natl Acad Sci USA 96 (7): 3611–5. doi:10.1073/pnas.96.7.3611. PMC 22342. PMID 10097085.

External links

• Methyl halide transferase at the US National Library of Medicine Medical Subject Headings (MeSH)