MicX sRNA

MicX Vibrio cholerae sRNA
Secondary structure of MicX sRNA.
Identifiers
Symbol MicX
Rfam RF01808
Other data
RNA type sRNA
Domain(s) Vibrio cholerae

MicX sRNA (formerly known as A10)[1] is a small non-coding RNA found in Vibrio cholerae.[2] It was given the name MicX as it has a similar function to MicA, MicC and MicF in E. coli.[3] MicX sRNA negatively regulates an outer membrane protein (coded for by VC0972) and also a component of an ABC transporter (gene VC0620).[4] These interactions were predicted and then confirmed using a DNA microarray.[2]

MicX was identified through a bioinformatics screen of V. cholerae having been previously predicted.[1] Levels of transcription of this sRNA were compared under several conditions: it was found to be expressed on all tested mediums; richer mediums slightly reduced transcription; repression of certain sigma factors (δS and δE) did not change transcription but it was dramatically reduced in the absence of Hfq protein.[2] This observation is in accordance with other sRNA expression patterns.[5][6]

The MicX RNA gene overlaps with VCA0943 - a gene coding for a maltose transporter permease - but the ribonuclease RNAase E processes the MicX transcript to create an active and stable form containing only the VCA0943 3' UTR.[2]

See also

References

  1. 1 2 Livny, Jonathan; Fogel, Michael A.; Davis, Brigid M.; Waldor, Matthew K. (2005). "sRNAPredict: an integrative computational approach to identify sRNAs in bacterial genomes". Nucleic Acids Research 33 (13): 4096–105. doi:10.1093/nar/gki715. PMC 1180744. PMID 16049021.
  2. 1 2 3 4 Davis, Brigid M.; Waldor, Matthew K. (2007). "RNase E-dependent processing stabilizes MicX, a Vibrio cholerae sRNA". Molecular Microbiology 65 (2): 373–85. doi:10.1111/j.1365-2958.2007.05796.x. PMC 1976385. PMID 17590231.
  3. ↑ Mizuno, T.; Chou, M. Y.; Inouye, M. (1984). "A unique mechanism regulating gene expression: translational inhibition by a complementary RNA transcript (micRNA)". Proceedings of the National Academy of Sciences 81 (7): 1966–70. doi:10.1073/pnas.81.7.1966. PMC 345417. PMID 6201848.
  4. ↑ Song, Tianyan; Mika, Franziska; Lindmark, Barbro; Liu, Zhi; Schild, Stefan; Bishop, Anne; Zhu, Jun; Camilli, Andrew; et al. (2008). "A new Vibrio cholerae sRNA modulates colonization and affects release of outer membrane vesicles". Molecular Microbiology 70 (1): 100–11. doi:10.1111/j.1365-2958.2008.06392.x. PMC 2628432. PMID 18681937.
  5. ↑ Sledjeski, Darren D.; Whitman, Christina; Zhang, Aixia (2001). "Hfq Is Necessary for Regulation by the Untranslated RNA DsrA". Journal of Bacteriology 183 (6): 1997–2005. doi:10.1128/JB.183.6.1997-2005.2001. PMC 95095. PMID 11222598.
  6. ↑ Massé, Eric; Escorcia, Freddy E.; Gottesman, Susan (2003). "Coupled degradation of a small regulatory RNA and its mRNA targets in Escherichia coli". Genes & Development 17 (19): 2374–83. doi:10.1101/gad.1127103. PMC 218075. PMID 12975324.

Further reading

External links

This article is issued from Wikipedia - version of the Friday, January 02, 2015. The text is available under the Creative Commons Attribution/Share Alike but additional terms may apply for the media files.