Nucleoporin 133

Nucleoporin 133kDa
PDB rendering based on 1xks.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1XKS​, 3CQC​, 3CQG​, 3I4R​
Identifiers
Symbols	NUP133 ; hNUP133
External IDs	OMIM: 607613 MGI: 2442620 HomoloGene: 32402 GeneCards: NUP133 Gene
Gene ontology Molecular function • nucleocytoplasmic transporter activity • protein binding Cellular component • condensed chromosome kinetochore • condensed chromosome outer kinetochore • nuclear envelope • nuclear pore • cytosol • membrane • nuclear pore outer ring • nuclear membrane Biological process • mitotic cell cycle • carbohydrate metabolic process • mRNA export from nucleus • nuclear pore organization • mitotic nuclear envelope disassembly • small GTPase mediated signal transduction • hexose transport • gene expression • regulation of glucose transport • protein transport • glucose transport • viral process • protein sumoylation • viral life cycle • viral transcription • cytokine-mediated signaling pathway • cellular response to heat • post-translational protein modification • cellular protein metabolic process • small molecule metabolic process • paraxial mesoderm development • transmembrane transport • regulation of cellular response to heat Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	55746	234865
Ensembl	ENSG00000069248	ENSMUSG00000039509
UniProt	Q8WUM0	Q8R0G9
RefSeq (mRNA)	NM_018230	NM_172288
RefSeq (protein)	NP_060700	NP_758492
Location (UCSC)	Chr 1: 229.44 – 229.51 Mb	Chr 8: 123.9 – 123.95 Mb
PubMed search

Nucleoporin 133 (Nup133) is a protein that in humans is encoded by the NUP133 gene.^[1][2]

Function

The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells.^[2]

Interactions

Nup133 has been shown to interact with NUP107.^[3][4]

References

1. Vasu S, Shah S, Orjalo A, Park M, Fischer WH, Forbes DJ (Oct 2001). "Novel vertebrate nucleoporins Nup133 and Nup160 play a role in mRNA export". The Journal of Cell Biology 155 (3): 339–54. doi:10.1083/jcb.200108007. PMC 2150853. PMID 11684705.
2. "Entrez Gene: NUP133 nucleoporin 133kDa".
3. Loïodice I, Alves A, Rabut G, Van Overbeek M, Ellenberg J, Sibarita JB, Doye V (Jul 2004). "The entire Nup107-160 complex, including three new members, is targeted as one entity to kinetochores in mitosis". Molecular Biology of the Cell 15 (7): 3333–44. doi:10.1091/mbc.E03-12-0878. PMC 452587. PMID 15146057.
4. Belgareh N, Rabut G, Baï SW, van Overbeek M, Beaudouin J, Daigle N, Zatsepina OV, Pasteau F, Labas V, Fromont-Racine M, Ellenberg J, Doye V (Sep 2001). "An evolutionarily conserved NPC subcomplex, which redistributes in part to kinetochores in mammalian cells". The Journal of Cell Biology 154 (6): 1147–60. doi:10.1083/jcb.200101081. PMC 2150808. PMID 11564755.

Further reading

• Stoffler D, Fahrenkrog B, Aebi U (Jun 1999). "The nuclear pore complex: from molecular architecture to functional dynamics". Current Opinion in Cell Biology 11 (3): 391–401. doi:10.1016/S0955-0674(99)80055-6. PMID 10395558.
• Bodoor K, Shaikh S, Enarson P, Chowdhury S, Salina D, Raharjo WH, Burke B (1999). "Function and assembly of nuclear pore complex proteins". Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire 77 (4): 321–9. doi:10.1139/bcb-77-4-321. PMID 10546895.
• Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA (Apr 1996). "A "double adaptor" method for improved shotgun library construction". Analytical Biochemistry 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID 8619474.
• Yu W, Andersson B, Worley KC, Muzny DM, Ding Y, Liu W, Ricafrente JY, Wentland MA, Lennon G, Gibbs RA (Apr 1997). "Large-scale concatenation cDNA sequencing". Genome Research 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174.
• Belgareh N, Rabut G, Baï SW, van Overbeek M, Beaudouin J, Daigle N, Zatsepina OV, Pasteau F, Labas V, Fromont-Racine M, Ellenberg J, Doye V (Sep 2001). "An evolutionarily conserved NPC subcomplex, which redistributes in part to kinetochores in mammalian cells". The Journal of Cell Biology 154 (6): 1147–60. doi:10.1083/jcb.200101081. PMC 2150808. PMID 11564755.
• Walther TC, Alves A, Pickersgill H, Loïodice I, Hetzer M, Galy V, Hülsmann BB, Köcher T, Wilm M, Allen T, Mattaj IW, Doye V (Apr 2003). "The conserved Nup107-160 complex is critical for nuclear pore complex assembly". Cell 113 (2): 195–206. doi:10.1016/S0092-8674(03)00235-6. PMID 12705868.
• Loïodice I, Alves A, Rabut G, Van Overbeek M, Ellenberg J, Sibarita JB, Doye V (Jul 2004). "The entire Nup107-160 complex, including three new members, is targeted as one entity to kinetochores in mitosis". Molecular Biology of the Cell 15 (7): 3333–44. doi:10.1091/mbc.E03-12-0878. PMC 452587. PMID 15146057.
• Berke IC, Boehmer T, Blobel G, Schwartz TU (Nov 2004). "Structural and functional analysis of Nup133 domains reveals modular building blocks of the nuclear pore complex". The Journal of Cell Biology 167 (4): 591–7. doi:10.1083/jcb.200408109. PMC 2172596. PMID 15557116.
• Nousiainen M, Silljé HH, Sauer G, Nigg EA, Körner R (Apr 2006). "Phosphoproteome analysis of the human mitotic spindle". Proceedings of the National Academy of Sciences of the United States of America 103 (14): 5391–6. doi:10.1073/pnas.0507066103. PMC 1459365. PMID 16565220.
• Beausoleil SA, Villén J, Gerber SA, Rush J, Gygi SP (Oct 2006). "A probability-based approach for high-throughput protein phosphorylation analysis and site localization". Nature Biotechnology 24 (10): 1285–92. doi:10.1038/nbt1240. PMID 16964243.
• Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (Nov 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983.
• Boehmer T, Schwartz TU (Sep 2007). "Purification, crystallization and preliminary X-ray analysis of a Nup107-Nup133 heterodimeric nucleoporin complex". Acta Crystallographica. Section F, Structural Biology and Crystallization Communications 63 (Pt 9): 816–8. doi:10.1107/S1744309107040523. PMC 2376328. PMID 17768364.