PIAS4

Protein inhibitor of activated STAT, 4
Identifiers
Symbols	PIAS4 ; PIAS-gamma; PIASY; Piasg; ZMIZ6
External IDs	OMIM: 605989 MGI: 2136940 HomoloGene: 104136 GeneCards: PIAS4 Gene
Gene ontology Molecular function • DNA binding • protein binding • protein C-terminus binding • zinc ion binding • ligase activity • SUMO transferase activity • ubiquitin protein ligase binding • SUMO ligase activity Cellular component • nucleus • nucleoplasm • cytoplasm • nuclear matrix • PML body • transferase complex Biological process • negative regulation of transcription from RNA polymerase II promoter • transcription, DNA-templated • negative regulation of tumor necrosis factor-mediated signaling pathway • Wnt signaling pathway • protein sumoylation • negative regulation of NF-kappaB transcription factor activity • positive regulation of protein sumoylation • post-translational protein modification • cellular protein metabolic process • negative regulation of transcription, DNA-templated • positive regulation of keratinocyte apoptotic process • positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	51588	59004
Ensembl	ENSG00000105229	ENSMUSG00000004934
UniProt	Q8N2W9	Q9JM05
RefSeq (mRNA)	NM_015897	NM_021501
RefSeq (protein)	NP_056981	NP_067476
Location (UCSC)	Chr 19: 4.01 – 4.04 Mb	Chr 10: 81.15 – 81.17 Mb
PubMed search

E3 SUMO-protein ligase PIAS4 is one of several protein inhibitor of activated STAT (PIAS) proteins. It is also known as protein inhibitor of activated STAT protein gamma (PIASg or PIASy), and is an enzyme that in humans is encoded by the PIAS4 gene.^[1][2]

Interactions

PIAS4 has been shown to interact with Mothers against decapentaplegic homolog 6,^[3] Mothers against decapentaplegic homolog 7^[3] and Lymphoid enhancer-binding factor 1.^[4]

References

1. Liu B, Liao J, Rao X, Kushner SA, Chung CD, Chang DD, Shuai K (Sep 1998). "Inhibition of Stat1-mediated gene activation by PIAS1". Proc Natl Acad Sci U S A 95 (18): 10626–31. doi:10.1073/pnas.95.18.10626. PMC 27945. PMID 9724754.
2. "Entrez Gene: PIAS4 Protein inhibitor of activated STAT, 4".
3. Imoto, Seiyu; Sugiyama Kenji; Muromoto Ryuta; Sato Noriko; Yamamoto Tetsuya; Matsuda Tadashi (Sep 2003). "Regulation of transforming growth factor-beta signaling by protein inhibitor of activated STAT, PIASy through Smad3". J. Biol. Chem. (United States) 278 (36): 34253–8. doi:10.1074/jbc.M304961200. ISSN 0021-9258. PMID 12815042.
4. Sachdev, S; Bruhn L; Sieber H; Pichler A; Melchior F; Grosschedl R (Dec 2001). "PIASy, a nuclear matrix-associated SUMO E3 ligase, represses LEF1 activity by sequestration into nuclear bodies". Genes Dev. (United States) 15 (23): 3088–103. doi:10.1101/gad.944801. ISSN 0890-9369. PMC 312834. PMID 11731474.

Further reading

• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Liu B, Gross M, ten Hoeve J, Shuai K (2001). "A transcriptional corepressor of Stat1 with an essential LXXLL signature motif". Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3203–7. doi:10.1073/pnas.051489598. PMC 30631. PMID 11248056.
• Nelson V, Davis GE, Maxwell SA (2001). "A putative protein inhibitor of activated STAT (PIASy) interacts with p53 and inhibits p53-mediated transactivation but not apoptosis". Apoptosis 6 (3): 221–34. doi:10.1023/A:1011392811628. PMID 11388671.
• Gross M, Liu B, Tan J, et al. (2001). "Distinct effects of PIAS proteins on androgen-mediated gene activation in prostate cancer cells". Oncogene 20 (29): 3880–7. doi:10.1038/sj.onc.1204489. PMID 11439351.
• Sachdev S, Bruhn L, Sieber H, et al. (2001). "PIASy, a nuclear matrix-associated SUMO E3 ligase, represses LEF1 activity by sequestration into nuclear bodies". Genes Dev. 15 (23): 3088–103. doi:10.1101/gad.944801. PMC 312834. PMID 11731474.
• Ohmine K, Ota J, Ueda M, et al. (2002). "Characterization of stage progression in chronic myeloid leukemia by DNA microarray with purified hematopoietic stem cells". Oncogene 20 (57): 8249–57. doi:10.1038/sj.onc.1205029. PMID 11781839.
• Tan JA, Hall SH, Hamil KG, et al. (2002). "Protein inhibitors of activated STAT resemble scaffold attachment factors and function as interacting nuclear receptor coregulators". J. Biol. Chem. 277 (19): 16993–7001. doi:10.1074/jbc.M109217200. PMID 11877418.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Subramanian L, Benson MD, Iñiguez-Lluhí JA (2003). "A synergy control motif within the attenuator domain of CCAAT/enhancer-binding protein alpha inhibits transcriptional synergy through its PIASy-enhanced modification by SUMO-1 or SUMO-3". J. Biol. Chem. 278 (11): 9134–41. doi:10.1074/jbc.M210440200. PMID 12511558.
• Dahle Ø, Andersen TØ, Nordgård O, et al. (2003). "Transactivation properties of c-Myb are critically dependent on two SUMO-1 acceptor sites that are conjugated in a PIASy enhanced manner". Eur. J. Biochem. 270 (6): 1338–48. doi:10.1046/j.1432-1033.2003.03504.x. PMID 12631292.
• Yamamoto H, Ihara M, Matsuura Y, Kikuchi A (2003). "Sumoylation is involved in beta-catenin-dependent activation of Tcf-4". EMBO J. 22 (9): 2047–59. doi:10.1093/emboj/cdg204. PMC 156076. PMID 12727872.
• Lee PS, Chang C, Liu D, Derynck R (2003). "Sumoylation of Smad4, the common Smad mediator of transforming growth factor-beta family signaling". J. Biol. Chem. 278 (30): 27853–63. doi:10.1074/jbc.M301755200. PMID 12740389.
• Chun TH, Itoh H, Subramanian L, et al. (2003). "Modification of GATA-2 transcriptional activity in endothelial cells by the SUMO E3 ligase PIASy". Circ. Res. 92 (11): 1201–8. doi:10.1161/01.RES.0000076893.70898.36. PMID 12750312.
• Imoto S, Sugiyama K, Muromoto R, et al. (2003). "Regulation of transforming growth factor-beta signaling by protein inhibitor of activated STAT, PIASy through Smad3". J. Biol. Chem. 278 (36): 34253–8. doi:10.1074/jbc.M304961200. PMID 12815042.
• Galleguillos D, Vecchiola A, Fuentealba JA, et al. (2004). "PIASgamma represses the transcriptional activation induced by the nuclear receptor Nurr1". J. Biol. Chem. 279 (3): 2005–11. doi:10.1074/jbc.M308113200. PMID 14559918.
• Gross M, Yang R, Top I, et al. (2004). "PIASy-mediated repression of the androgen receptor is independent of sumoylation". Oncogene 23 (17): 3059–66. doi:10.1038/sj.onc.1207443. PMID 14981544.
• Zoumpoulidou G, Jones MC, Fernandez de Mattos S, et al. (2005). "Convergence of interferon-gamma and progesterone signaling pathways in human endometrium: role of PIASy (protein inhibitor of activated signal transducer and activator of transcription-y)". Mol. Endocrinol. 18 (8): 1988–99. doi:10.1210/me.2003-0467. PMID 15155784.
• Imoto S, Sugiyama K, Yamamoto T, Matsuda T (2004). "The RING domain of PIASy is involved in the suppression of bone morphogenetic protein-signaling pathway". Biochem. Biophys. Res. Commun. 319 (1): 275–82. doi:10.1016/j.bbrc.2004.04.161. PMID 15158472.