POLRMT

Polymerase (RNA) mitochondrial (DNA directed)
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 3SPA​, 4BOC​
Identifiers
Symbols	POLRMT ; APOLMT; MTRNAP; MTRPOL; h-mtRPOL
External IDs	OMIM: 601778 MGI: 1915843 HomoloGene: 37996 GeneCards: POLRMT Gene
EC number	2.7.7.6
Gene ontology Molecular function • DNA binding • DNA-directed RNA polymerase activity • protein binding • poly(A) RNA binding Cellular component • mitochondrion • mitochondrial matrix • mitochondrial nucleoid Biological process • transcription from mitochondrial promoter • transcription initiation from mitochondrial promoter • organelle organization • mitochondrion organization • gene expression Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	5442	216151
Ensembl	ENSG00000099821	ENSMUSG00000020329
UniProt	O00411	Q8BKF1
RefSeq (mRNA)	NM_005035	NM_172551
RefSeq (protein)	NP_005026	NP_766139
Location (UCSC)	Chr 19: 0.62 – 0.63 Mb	Chr 10: 79.74 – 79.75 Mb
PubMed search

DNA-directed RNA polymerase, mitochondrial is an enzyme that in humans is encoded by the POLRMT gene.^[1][2]

Function

This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of bacteriophage and mitochondrial polymerases of lower eukaryotes.^[2]

References

1. Tiranti V, Savoia A, Forti F, D'Apolito MF, Centra M, Rocchi M, Zeviani M (Jul 1997). "Identification of the gene encoding the human mitochondrial RNA polymerase (h-mtRPOL) by cyberscreening of the Expressed Sequence Tags database". Hum Mol Genet 6 (4): 615–25. doi:10.1093/hmg/6.4.615. PMID 9097968.
2. "Entrez Gene: POLRMT polymerase (RNA) mitochondrial (DNA directed)".

Further reading

• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Falkenberg M, Gaspari M, Rantanen A, et al. (2002). "Mitochondrial transcription factors B1 and B2 activate transcription of human mtDNA". Nat. Genet. 31 (3): 289–94. doi:10.1038/ng909. PMID 12068295.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• McCulloch V, Shadel GS (2003). "Human mitochondrial transcription factor B1 interacts with the C-terminal activation region of h-mtTFA and stimulates transcription independently of its RNA methyltransferase activity". Mol. Cell. Biol. 23 (16): 5816–24. doi:10.1128/MCB.23.16.5816-5824.2003. PMC 166325. PMID 12897151.
• Grimwood J, Gordon LA, Olsen A, et al. (2004). "The DNA sequence and biology of human chromosome 19". Nature 428 (6982): 529–35. doi:10.1038/nature02399. PMID 15057824.
• Graziewicz MA, Longley MJ, Bienstock RJ, et al. (2004). "Structure-function defects of human mitochondrial DNA polymerase in autosomal dominant progressive external ophthalmoplegia". Nat. Struct. Mol. Biol. 11 (8): 770–6. doi:10.1038/nsmb805. PMID 15258572.
• Gaspari M, Falkenberg M, Larsson NG, Gustafsson CM (2005). "The mitochondrial RNA polymerase contributes critically to promoter specificity in mammalian cells". EMBO J. 23 (23): 4606–14. doi:10.1038/sj.emboj.7600465. PMC 533051. PMID 15526033.
• Kravchenko JE, Rogozin IB, Koonin EV, Chumakov PM (2005). "Transcription of mammalian messenger RNAs by a nuclear RNA polymerase of mitochondrial origin". Nature 436 (7051): 735–9. doi:10.1038/nature03848. PMC 1352165. PMID 16079853.
• Wang Z, Cotney J, Shadel GS (2007). "Human mitochondrial ribosomal protein MRPL12 interacts directly with mitochondrial RNA polymerase to modulate mitochondrial gene expression". J. Biol. Chem. 282 (17): 12610–8. doi:10.1074/jbc.M700461200. PMC 2606046. PMID 17337445.