PRPF6

Pre-mRNA processing factor 6
Identifiers
Symbols	PRPF6 ; ANT-1; ANT1; C20orf14; Prp6; RP60; SNRNP102; TOM; U5-102K; hPrp6
External IDs	OMIM: 613979 MGI: 1922946 HomoloGene: 5368 GeneCards: PRPF6 Gene
Gene ontology Molecular function • transcription coactivator activity • RNA binding • protein binding • ribonucleoprotein complex binding • poly(A) RNA binding • androgen receptor binding Cellular component • nucleus • nucleoplasm • spliceosomal complex • U5 snRNP • membrane • nuclear speck • U4/U6 x U5 tri-snRNP complex • catalytic step 2 spliceosome Biological process • spliceosomal tri-snRNP complex assembly • spliceosomal complex assembly • RNA splicing, via transesterification reactions • mRNA splicing, via spliceosome • RNA localization • RNA splicing • gene expression • positive regulation of transcription from RNA polymerase II promoter Sources: Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	24148	68879
Ensembl	ENSG00000101161	ENSMUSG00000002455
UniProt	O94906	Q91YR7
RefSeq (mRNA)	NM_012469	NM_133701
RefSeq (protein)	NP_036601	NP_598462
Location (UCSC)	Chr 20: 63.98 – 64.03 Mb	Chr 2: 181.59 – 181.66 Mb
PubMed search

Pre-mRNA-processing factor 6 is a protein that in humans is encoded by the PRPF6 gene.^[1][2][3]

The protein encoded by this gene appears to be involved in pre-mRNA splicing, possibly acting as a bridging factor between U5 and U4/U6 snRNPs in formation of the spliceosome. The encoded protein also can bind androgen receptor, providing a link between transcriptional activation and splicing.^[3]

Interactions

PRPF6 has been shown to interact with TXNL4B,^[4] ARAF^[5][6] and Androgen receptor.^[7]

References

1. Nishikimi A, Mukai J, Kioka N, Yamada M (January 2000). "A novel mammalian nuclear protein similar to Schizosaccharomyces pombe Prp1p/Zer1p and Saccharomyces cerevisiae Prp6p pre-mRNA splicing factors". Biochim Biophys Acta 1435 (1-2): 147–52. doi:10.1016/s0167-4838(99)00203-4. PMID 10561546.
2. Makarov EM, Makarova OV, Achsel T, Luhrmann R (June 2000). "The human homologue of the yeast splicing factor prp6p contains multiple TPR elements and is stably associated with the U5 snRNP via protein-protein interactions". J Mol Biol 298 (4): 567–75. doi:10.1006/jmbi.2000.3685. PMID 10788320.
3. "Entrez Gene: PRPF6 PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae)".
4. Sun, Xiaojing; Zhang Hua; Wang Dan; Ma Dalong; Shen Yan; Shang Yongfeng (July 2004). "DLP, a novel Dim1 family protein implicated in pre-mRNA splicing and cell cycle progression". J. Biol. Chem. (United States) 279 (31): 32839–47. doi:10.1074/jbc.M402522200. ISSN 0021-9258. PMID 15161931.
5. Yuryev, Anton; Wennogle Lawrence P (February 2003). "Novel raf kinase protein-protein interactions found by an exhaustive yeast two-hybrid analysis". Genomics (United States) 81 (2): 112–25. doi:10.1016/S0888-7543(02)00008-3. ISSN 0888-7543. PMID 12620389.
6. Yuryev, A; Ono M; Goff S A; Macaluso F; Wennogle L P (July 2000). "Isoform-specific localization of A-RAF in mitochondria". Mol. Cell. Biol. (UNITED STATES) 20 (13): 4870–8. doi:10.1128/MCB.20.13.4870-4878.2000. ISSN 0270-7306. PMC 85938. PMID 10848612.
7. Zhao, Yue; Goto Kiminobu; Saitoh Masayuki; Yanase Toshihiko; Nomura Masatoshi; Okabe Taijiro; Takayanagi Ryoichi; Nawata Hajime (August 2002). "Activation function-1 domain of androgen receptor contributes to the interaction between subnuclear splicing factor compartment and nuclear receptor compartment. Identification of the p102 U5 small nuclear ribonucleoprotein particle-binding protein as a coactivator for the receptor". J. Biol. Chem. (United States) 277 (33): 30031–9. doi:10.1074/jbc.M203811200. ISSN 0021-9258. PMID 12039962.

Further reading

• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1-2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library.". Gene 200 (1-2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Yuryev A, Ono M, Goff SA, et al. (2000). "Isoform-specific localization of A-RAF in mitochondria.". Mol. Cell. Biol. 20 (13): 4870–8. doi:10.1128/MCB.20.13.4870-4878.2000. PMC 85938. PMID 10848612.
• Deloukas P, Matthews LH, Ashurst J, et al. (2002). "The DNA sequence and comparative analysis of human chromosome 20.". Nature 414 (6866): 865–71. doi:10.1038/414865a. PMID 11780052.
• Jurica MS, Licklider LJ, Gygi SR, et al. (2002). "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis.". RNA 8 (4): 426–39. doi:10.1017/S1355838202021088. PMC 1370266. PMID 11991638.
• Zhao Y, Goto K, Saitoh M, et al. (2002). "Activation function-1 domain of androgen receptor contributes to the interaction between subnuclear splicing factor compartment and nuclear receptor compartment. Identification of the p102 U5 small nuclear ribonucleoprotein particle-binding protein as a coactivator for the receptor.". J. Biol. Chem. 277 (33): 30031–9. doi:10.1074/jbc.M203811200. PMID 12039962.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Yuryev A, Wennogle LP (2003). "Novel raf kinase protein-protein interactions found by an exhaustive yeast two-hybrid analysis.". Genomics 81 (2): 112–25. doi:10.1016/S0888-7543(02)00008-3. PMID 12620389.
• Goto K, Zhao Y, Saito M, et al. (2003). "Activation function-1 domain of androgen receptor contributes to the interaction between two distinct subnuclear compartments.". J. Steroid Biochem. Mol. Biol. 85 (2-5): 201–8. doi:10.1016/S0960-0760(03)00196-1. PMID 12943705.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Will CL, Schneider C, Hossbach M, et al. (2004). "The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome.". RNA 10 (6): 929–41. doi:10.1261/rna.7320604. PMC 1370585. PMID 15146077.
• Sun X, Zhang H, Wang D, et al. (2004). "DLP, a novel Dim1 family protein implicated in pre-mRNA splicing and cell cycle progression.". J. Biol. Chem. 279 (31): 32839–47. doi:10.1074/jbc.M402522200. PMID 15161931.
• Suzuki Y, Yamashita R, Shirota M, et al. (2004). "Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions.". Genome Res. 14 (9): 1711–8. doi:10.1101/gr.2435604. PMC 515316. PMID 15342556.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Laggerbauer B, Liu S, Makarov E, et al. (2005). "The human U5 snRNP 52K protein (CD2BP2) interacts with U5-102K (hPrp6), a U4/U6.U5 tri-snRNP bridging protein, but dissociates upon tri-snRNP formation.". RNA 11 (5): 598–608. doi:10.1261/rna.2300805. PMC 1370748. PMID 15840814.
• Batsché E, Yaniv M, Muchardt C (2006). "The human SWI/SNF subunit Brm is a regulator of alternative splicing.". Nat. Struct. Mol. Biol. 13 (1): 22–9. doi:10.1038/nsmb1030. PMID 16341228.
• Fan S, Goto K, Chen G, et al. (2006). "Identification of the functional domains of ANT-1, a novel coactivator of the androgen receptor.". Biochem. Biophys. Res. Commun. 341 (1): 192–201. doi:10.1016/j.bbrc.2005.12.167. PMID 16414017.
• Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry.". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.

External links

• GeneReviews/NCBI/NIH/UW entry on Retinitis Pigmentosa Overview