Phikmvlikevirus
| Phikmvlikevirus | |
|---|---|
| Virus classification | |
| Group: | Group I (dsDNA) |
| Order: | Caudovirales |
| Family: | Podoviridae |
| Subfamily: | Autographivirinae |
| Genus: | Phikmvlikevirus |
| Type Species | |
| |
Phikmvlikevirus is a genus of viruses in the order Caudovirales, in the family Podoviridae, in the subfamily Autographivirinae. Bacteria serve as natural hosts. There are currently four species in this genus including the type species Pseudomonas phage phiKMV.[1][2] Bacteriophage phiKMV[3] and its relatives are known to be highly virulent phages, producing large (3–15 mm (0.12–0.59 in) diameter) clear plaques upon plating on a susceptible host.[4][5] The only reported exception is phage LKA1, which yields small plaques (1 mm (0.039 in)) surrounded by a halo. While all other P. aeruginosa-specific phikmvlikeviruses use the Type IV pili as primary receptor, LKA1 particles attach to the bacterial lipopolysacharide layer.
Taxonomy
Group: dsDNA
- Family: Podoviridae
- Sub-Family: Autographivirinae
- Genus: Phikmvlikevirus
- Pantoea phage Limelight
- Pantoea phage Limezero
- Pseudomonas phage LKA1
- Pseudomonas phage phiKMV
Virology
Electron microscopic imaging of purified phage particles revealed these phages as typical members of the Podoviridae, with a head diameter of approximately 60 nm (2.4×10−6 in) and a stubby tail with a length of 8–10 nm. Although phiKMV phage resembles the well-studied podovirus T7 in overall genome architecture, it was the first known T7-like phage which encoded a single-subunit RNA polymerase gene downstream its DNA metabolism genes instead of in the early genomic region. Based on these properties, the genus Phikmvlikevirus is classified within the Autographivirinae.[6]
| Genus | Structure | Symmetry | Capsid | Genomic Arrangement | Genomic Segmentation |
|---|---|---|---|---|---|
| Phikmvlikevirus | Head-Tail | T=7 | Non-Enveloped | Linear | Monopartite |
Life Cycle
Viral replication is cytoplasmic. Entry into the host cell is achieved by adsorption into the host cell. DNA-templated transcription is the method of transcription. The virus exits the host cell by lysis, and holin/endolysin/spanin proteins. Bacteria serve as the natural host. Transmission routes are passive diffusion.[1]
| Genus | Host Details | Tissue Tropism | Entry Details | Release Details | Replication Site | Assembly Site | Transmission |
|---|---|---|---|---|---|---|---|
| Phikmvlikevirus | Bacteria | None | Injection | Lysis | Cytoplasm | Cytoplasm | Passive diffusion |
References
- 1 2 "Viral Zone". ExPASy. Retrieved 15 June 2015.
- 1 2 ICTV. "Virus Taxonomy: 2014 Release". Retrieved 15 June 2015.
- ↑ Lavigne, Rob; Burkal'tseva, Maria V.; Robben, Johan; Sykilinda, Nina N.; Kurochkina, Lidia P.; Grymonprez, Barbara; Jonckx, Bart; Krylov, Victor N.; et al. (2003). "The genome of bacteriophage φKMV, a T7-like virus infecting Pseudomonas aeruginosa". Virology 312 (1): 49–59. doi:10.1016/S0042-6822(03)00123-5. PMID 12890620.
- ↑ Ceyssens, P.-J.; Lavigne, R.; Mattheus, W.; Chibeu, A.; Hertveldt, K.; Mast, J.; Robben, J.; Volckaert, G. (2006). "Genomic Analysis of Pseudomonas aeruginosa Phages LKD16 and LKA1: Establishment of the KMV Subgroup within the T7 Supergroup". Journal of Bacteriology 188 (19): 6924–31. doi:10.1128/JB.00831-06. PMC 1595506. PMID 16980495.
- ↑ Lammens, E.; Ceyssens, P.-J.; Voet, M.; Hertveldt, K.; Lavigne, R.; Volckaert, G. (2009). "Representational Difference Analysis (RDA) of bacteriophage genomes". Journal of Microbiological Methods 77 (2): 207–13. doi:10.1016/j.mimet.2009.02.006. PMID 19232531.
- ↑ Lavigne, Rob; Seto, Donald; Mahadevan, Padmanabhan; Ackermann, Hans-W.; Kropinski, Andrew M. (2008). "Unifying classical and molecular taxonomic classification: analysis of the Podoviridae using BLASTP-based tools". Research in Microbiology 159 (5): 406–14. doi:10.1016/j.resmic.2008.03.005. PMID 18555669.