Ribosome-nascent chain complex

Visualisation of an RNC showing the ribosome as a translucent cartoon and the nascent chain as a pink ribbon

Ribosome-nascent chain complex (RNC) refers to the collection of molecules that constitute a ribosome attached to the polypeptide (protein) that it is synthesising. The synthesis of the nascent polypeptide may be stalled by one of several methods. RNCs are produced and purified in laboratories to study the dynamics, biochemistry, folding and interactions of both the ribosome and proteins undergoing synthesis.^[1]^[2]

References

↑ Cabrita, Lisa D.; Dobson, Christopher M.; Christodoulou, John (2010). "Protein folding on the ribosome". Current Opinion in Structural Biology 20: 33–45. doi:10.1016/j.sbi.2010.01.005.
↑ Schaffitzel, Christiane; Ban, Nenad (2007). "Generation of ribosome nascent chain complexes for structural and functional studies". Journal of Structural Biology 158: 463–471. doi:10.1016/j.jsb.2007.01.005.

Structures of the cell / organelles (TH H1.00.01.2-3)

Endomembrane system	Cell membrane Nucleus Nucleolus Endoplasmic reticulum Golgi apparatus Parenthesome Autophagosome Vesicles Exosome Lysosome Endosome Phagosome Vacuole Acrosome Cytoplasmic granules Melanosome Microbody Glyoxysome Peroxisome Weibel–Palade body

Cytoskeleton	Microfilaments Intermediate filaments Microtubules Prokaryotic cytoskeleton MTOCs Centrosome Centriole Basal body Spindle pole body Myofibril

Endosymbionts	Mitochondrion Plastids Chloroplast Chromoplast Gerontoplast Leucoplast Amyloplast Elaioplast Proteinoplast Tannosome

Other internal	RNA Ribosome Spliceosome Vault Cytoplasm Proteasome

External	Undulipodium Cilium Flagellum Axoneme Radial spoke Extracellular matrix Cell wall

Ribosomal RNA / ribosome subunits

Prokaryotes (70S)

Large

50S

23S

Small

30S

16S

Eukaryotes (80S)

Cytoplasmic	Large 60S 5S 5.8S 28S Small 40S 18S

Mitochondrial	MT-RNR1, 12S MT-RNR2, 16S

Gene expression

Introduction
to genetics

Transcription

Types	Bacterial Eukaryotic

Key elements	Transcription factor RNA polymerase Promoter

Post-transcription	Precursor mRNA (pre-mRNA / hnRNA) 5' capping Splicing Polyadenylation Histone acetylation and deacetylation

Translation

Types	Prokaryotic Eukaryotic

Key elements	Ribosome Transfer RNA (tRNA) Ribosome-nascent chain complex (RNC) Post-translational modification (functional groups · peptides · structural changes)

Regulation

Proteins: key methods of study

Experimental	Protein purification Green fluorescent protein Western blot Protein immunostaining Protein sequencing Gel electrophoresis/Protein electrophoresis Protein immunoprecipitation Peptide mass fingerprinting/Protein mass spectrometry Dual-polarization interferometry Microscale thermophoresis Chromatin immunoprecipitation Surface plasmon resonance Isothermal titration calorimetry X-ray crystallography Protein NMR Cryo-electron microscopy Freeze-fracture electron microscopy

Bioinformatics	Protein structure prediction Protein–protein docking Protein structural alignment Protein ontology Protein–protein interaction prediction

Assay	Enzyme assay Protein assay Secretion assay

Display techniques	Bacterial display mRNA display Phage display Ribosome display Yeast display

Super-resolution microscopy	Photoactivated localization microscopy Vertico SMI

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