SNAPC4

Small nuclear RNA activating complex, polypeptide 4, 190kDa
Identifiers
Symbols	SNAPC4 ; PTFalpha; SNAP190
External IDs	OMIM: 602777 MGI: 2443935 HomoloGene: 2321 GeneCards: SNAPC4 Gene
Gene ontology Molecular function • DNA binding • transcription factor activity, sequence-specific DNA binding Cellular component • nucleus • nucleoplasm • snRNA-activating protein complex Biological process • regulation of transcription, DNA-templated • transcription from RNA polymerase II promoter • transcription from RNA polymerase III promoter • snRNA transcription • gene expression • snRNA transcription from RNA polymerase II promoter • snRNA transcription from RNA polymerase III promoter Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	6621	227644
Ensembl	ENSG00000165684	ENSMUSG00000036281
UniProt	Q5SXM2	Q8BP86
RefSeq (mRNA)	NM_003086	NM_001290419
RefSeq (protein)	NP_003077	NP_001277348
Location (UCSC)	Chr 9: 136.38 – 136.4 Mb	Chr 2: 26.36 – 26.38 Mb
PubMed search

snRNA-activating protein complex subunit 4 is a protein that in humans is encoded by the SNAPC4 gene.^[1][2]

Model organisms

Snapc4 knockout mouse phenotype

Characteristic	Phenotype
Homozygote viability	Abnormal
Recessive lethal study	Abnormal
Fertility	Normal
Body weight	Normal
Anxiety	Normal
Neurological assessment	Normal
Grip strength	Normal
Hot plate	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Body temperature	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Plasma immunoglobulins	Normal
Haematology	Normal
Peripheral blood lymphocytes	Normal
Micronucleus test	Normal
Heart weight	Normal
Skin Histopathology	Normal
Brain histopathology	Normal
Salmonella infection	Normal[3]
Citrobacter infection	Normal[4]
All tests and analysis from[5][6]

Model organisms have been used in the study of SNAPC4 function. A conditional knockout mouse line, called Snapc4^{tm1a(KOMP)Wtsi[7][8]} was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists — at the Wellcome Trust Sanger Institute.^[9][10][11]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.^[5][12] Twenty six tests were carried out on mutant mice and two significant abnormalities were observed. No homozygous mutant embryos were recorded during gestation and, in a separate study, no homozygous animals were observed at weaning. The remaining tests were carried out on adult heterozygous mutant animals and no further abnormalities were observed.^[5]

Interactions

SNAPC4 has been shown to interact with SNAPC1,^[13] POU2F1^[1][14] and SNAPC2.^[1][13]

References

1. Wong MW, Henry RW, Ma B, Kobayashi R, Klages N, Matthias P, Strubin M, Hernandez N (January 1998). "The Large Subunit of Basal Transcription Factor SNAPc Is a Myb Domain Protein That Interacts with Oct-1". Mol Cell Biol 18 (1): 368–77. PMC 121507. PMID 9418884.
2. "Entrez Gene: SNAPC4 small nuclear RNA activating complex, polypeptide 4, 190kDa".
3. "Salmonella infection data for Snapc4". Wellcome Trust Sanger Institute.
4. "Citrobacter infection data for Snapc4". Wellcome Trust Sanger Institute.
5. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica 88 (S248). doi:10.1111/j.1755-3768.2010.4142.x.
6. Mouse Resources Portal, Wellcome Trust Sanger Institute.
7. "International Knockout Mouse Consortium".
8. "Mouse Genome Informatics".
9. Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–342. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
10. Dolgin E (June 2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
11. Collins FS, Rossant J, Wurst W (January 2007). "A mouse for all reasons". Cell 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
12. van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism.". Genome Biol 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.
13. Ma, B; Hernandez N (February 2001). "A map of protein-protein contacts within the small nuclear RNA-activating protein complex SNAPc". J. Biol. Chem. (United States) 276 (7): 5027–35. doi:10.1074/jbc.M009301200. ISSN 0021-9258. PMID 11056176.
14. Hovde, Stacy; Brooks Aimee; Strong Katie; Geiger James H (March 2002). "Crystallization of the Oct-1/SNAP190 peptide/DNA complex". Acta Crystallogr. D Biol. Crystallogr. (Denmark) 58 (Pt 3): 511–2. doi:10.1107/S0907444901021461. ISSN 0907-4449. PMID 11856838.

Further reading

• Henry RW, Sadowski CL, Kobayashi R, Hernandez N (1995). "A TBP-TAF complex required for transcription of human snRNA genes by RNA polymerase II and III". Nature 374 (6523): 653–6. doi:10.1038/374653a0. PMID 7715707.
• Ma B, Hernandez N (2001). "A map of protein-protein contacts within the small nuclear RNA-activating protein complex SNAPc". J. Biol. Chem. 276 (7): 5027–35. doi:10.1074/jbc.M009301200. PMID 11056176.
• Hovde S, Brooks A, Strong K, Geiger JH (2002). "Crystallization of the Oct-1/SNAP190 peptide/DNA complex". Acta Crystallogr. D Biol. Crystallogr. 58 (Pt 3): 511–2. doi:10.1107/S0907444901021461. PMID 11856838.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Hinkley CS, Hirsch HA, Gu L, et al. (2003). "The small nuclear RNA-activating protein 190 Myb DNA binding domain stimulates TATA box-binding protein-TATA box recognition". J. Biol. Chem. 278 (20): 18649–57. doi:10.1074/jbc.M204247200. PMID 12621023.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Humphray SJ, Oliver K, Hunt AR, et al. (2004). "DNA sequence and analysis of human chromosome 9". Nature 429 (6990): 369–74. doi:10.1038/nature02465. PMC 2734081. PMID 15164053.
• Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Gu L, Esselman WJ, Henry RW (2005). "Cooperation between small nuclear RNA-activating protein complex (SNAPC) and TATA-box-binding protein antagonizes protein kinase CK2 inhibition of DNA binding by SNAPC". J. Biol. Chem. 280 (30): 27697–704. doi:10.1074/jbc.M503206200. PMID 15955816.
• Gu L, Husain-Ponnampalam R, Hoffmann-Benning S, Henry RW (2007). "The protein kinase CK2 phosphorylates SNAP190 to negatively regulate SNAPC DNA binding and human U6 transcription by RNA polymerase III". J. Biol. Chem. 282 (38): 27887–96. doi:10.1074/jbc.M702269200. PMID 17670747.