Triticale

Triticale
Triticale
Scientific classification
Kingdom: Plantae
(unranked): Angiosperms
(unranked): Monocots
(unranked): Commelinids
Order: Poales
Family: Poaceae
Tribe: Triticeae
Genus: × Triticosecale
Binomial name
× Triticosecale
Wittm. ex A. Camus.
Synonyms

× Triticale Tscherm.-Seys. ex Müntzing

Triticale (× Triticosecale), /trɪtɪˈkeɪliː/ is a hybrid of wheat (Triticum) and rye (Secale) first bred in laboratories during the late 19th century in Scotland and Sweden. Commercially available triticale is almost always a second-generation hybrid, i.e., a cross between two kinds of primary (first-cross) triticales. As a rule, triticale combines the yield potential and grain quality of wheat with the disease and environmental tolerance (including soil conditions) of rye. Only recently has it been developed into a commercially viable crop. Depending on the cultivar, triticale can more or less resemble either of its parents. It is grown mostly for forage or fodder, although some triticale-based foods can be purchased at health food stores or are to be found in some breakfast cereals.

When crossing wheat and rye, wheat is used as the female parent and rye as the male parent (pollen donor). The resulting hybrid is sterile and must be treated with colchicine to induce polyploidy and thus the ability to reproduce itself.

The primary producers of triticale are Poland, Germany, Belarus, France and Russia. In 2014, according to the Food and Agriculture Organization (FAO), 17.1 million tons were harvested in 37 countries across the world.[1]

The triticale hybrids are all amphidiploid, which means the plant is diploid for two genomes derived from different species. In other words, triticale is an allotetraploid. In earlier years, most work was done on octoploid triticale. Different ploidy levels have been created and evaluated over time. The tetraploids showed little promise, but hexaploid triticale was successful enough to find commercial application.[2]

The International Maize and Wheat Improvement Center triticale improvement program wanted to improve food production and nutrition in developing countries. Triticale has potential in the production of bread and other food products, such as cookies, pasta, pizza dough and breakfast cereals.[2] The protein content is higher than that of wheat, although the glutenin fraction is less. The grain has also been stated to have higher levels of lysine than wheat.[3] Assuming increased acceptance, the milling industry will have to adapt to triticale, as the milling techniques employed for wheat are unsuited to triticale. Sell et al.[4] found triticale could be used as a feed grain, and later research found its starch was particularly readily digested.[5] As a feed grain, triticale is already well established and of high economic importance. It has received attention as a potential energy crop, and research is currently being conducted on the use of the crop's biomass in bioethanol production.

Biology and genetics

The grain of wheat, rye and triticale — triticale grain is significantly larger than that of wheat.

Earlier work with wheat-rye crosses was difficult due to low survival of the resulting hybrid embryo and spontaneous chromosome doubling. These two factors were difficult to predict and control. To improve the viability of the embryo and thus avoid its abortion, in vitro culture techniques were developed (Laibach, 1925). Colchicine was used as a chemical agent to double the chromosomes (Blakeslee & Avery 1937). After these developments, a new era of triticale breeding was introduced. Earlier triticale hybrids had four reproductive disorders—namely, meiotic instability, high aneuploid frequency, low fertility and shriveled seed (Muntzing 1939; Krolow 1966). Cytogenetical studies were encouraged and well funded to overcome these problems.

It is especially difficult to see the expression of rye genes in the background of wheat cytoplasm and the predominant wheat nuclear genome. This makes it difficult to realise the potential of rye in disease resistance and ecological adaptation. One of the ways to relieve this problem was to produce secalotricum, in which rye cytoplasm was used instead of that from wheat.

Triticale is essentially a self-fertilizing, or naturally inbred, crop. This mode of reproduction results in a more homozygous genome. The crop is, however, adapted to this form of reproduction from an evolutionary point of view. Cross-fertilization is also possible, but it is not the primary form of reproduction.

Conventional breeding approaches

Top Triticale producers
in 2014
(million metric tons)
 Poland 5.2
 Germany 3.0
 Belgium 2.1
 France 2.0
 Russia 0.7
 China 0.5
 Hungary 0.5
 Spain 0.4
 Lithuania 0.4
 Austria 0.3
World total 17.1
Source:
UN Food & Agriculture Organisation
(FAO)

The aim of a triticale breeding programme is mainly focused on the improvement of quantitative traits, such as grain yield, nutritional quality and plant height, as well as traits which are more difficult to improve, such as earlier maturity and improved test weight (a measure of bulk density). These traits are controlled by more than one gene.[6] Problems arise, however, because such polygenic traits involve the integration of several physiological processes in their expression. Thus the lack of single-gene control (or simple inheritance) results in low trait heritability (Zumelzú et al. 1998).

Since the induction of the CIMMYT triticale breeding programme in 1964, the improvement in realised grain yield has been remarkable. In 1968, at Ciudad Obregón, Sonora, in northwest Mexico, the highest yielding triticale line produced 2.4 t/ha. Today, CIMMYT has released high yielding spring triticale lines (e.g. Pollmer-2) which have surpassed the 10 t/ha yield barrier under optimum production conditions.[7]

Based on the commercial success of other hybrid crops, the use of hybrid triticales as a strategy for enhancing yield in favourable, as well as marginal, environments has proven successful over time. Earlier research conducted by CIMMYT made use of a chemical hybridising agent to evaluate heterosis in hexaploid triticale hybrids. To select the most promising parents for hybrid production, test crosses conducted in various environments are required, because the variance of their specific combining ability under differing environmental conditions is the most important component in evaluating their potential as parents to produce promising hybrids. The prediction of general combining ability of any triticale plant from the performance of its parents is only moderate with respect to grain yield. Commercially exploitable yield advantages of hybrid triticale cultivars is dependent on improving parent heterosis and on advances in inbred-line development.

Triticale is useful as an animal feed grain. However, it is necessary to improve its milling and bread-making quality aspects to increase its potential for human consumption. The relationship between the constituent wheat and rye genomes were noted to produce meiotic irregularities, and genome instability and incompatibility presented numerous problems when attempts were made to improve triticale. This led to two alternative methods to study and improve its reproductive performance, namely, the improvement of the number of grains per floral spikelet and its meiotic behaviour. The number of grains per spikelet has an associated low heritability value (de Zumelzú et al. 1998). In improving yield, indirect selection (the selection of correlated/related traits other than that to be improved) is not necessarily as effective as direct selection. (Gallais 1984)[8]

Lodging (the toppling over of the plant stem, especially under windy conditions) resistance is a complexly inherited (expression is controlled by many genes) trait, and has thus been an important breeding aim in the past.[9] The use of dwarfing genes, known as Rht genes, which have been incorporated from both Triticum and Secale, has resulted in a decrease of up to 20 cm in plant height without causing any adverse effects.

Application of newer techniques

Abundant information exists concerning disease resistance (R) genes for wheat, and a continuously updated on-line catalogue, the Catalogue of Gene Symbols, of these genes can be found at . Another on-line database of cereal rust resistance genes is available at . Unfortunately, less is known about rye and particularly triticale R-genes. Many R-genes have been transferred to wheat from its wild relatives, and appear in the catalogue, thus making them available for triticale breeding. The two mentioned databases are significant contributors to improving the genetic variability of the triticale gene pool through gene (or more specifically, allele) provision. Genetic variability is essential for progress in breeding. In addition, genetic variability can also be achieved by producing new primary triticales, which essentially means the reconstitution of triticale, and the development of various hybrids involving triticale, such as triticale-rye hybrids. In this way, some chromosomes from the R genome have been replaced by some from the D genome. The resulting so-called substitution and translocation triticale facilitates the transfer of R-genes.

Introgression

Introgression involves the crossing of closely related plant relatives, and results in the transfer of 'blocks' of genes, i.e. larger segments of chromosomes compared to single genes. R-genes are generally introduced within such blocks, which are usually incorporated/translocated/introgressed into the distal (extreme) regions of chromosomes of the crop being introgressed. Genes located in the proximal areas of chromosomes may be completely linked (very closely spaced), thus preventing or severely hampering genetic recombination, which is necessary to incorporate such blocks.[10] Molecular markers (small lengths of DNA of a characterized/known sequence) are used to 'tag' and thus track such translocations. [11] A weak colchicine solution has been employed to increase the probability of recombination in the proximal chromosome regions, and thus the introduction of the translocation to that region. The resultant translocation of smaller blocks that indeed carry the R-gene(s) of interest has decreased the probability of introducing unwanted genes.[12]

Production of doubled haploids

Doubled haploid (DH) plants have the potential to save much time in the development of inbred lines. This is achieved in a single generation, as opposed to many, which would otherwise occupy much physical space/facilities. DHs also express deleterious recessive alleles otherwise masked by dominance effects in a genome containing more than one copy of each chromosome (and thus more than one copy of each gene). Various techniques exist to create DHs. The in vitro culture of anthers and microspores is most often used in cereals, including triticale.[13][14][15] These two techniques are referred to as androgenesis, which refers to the development of pollen. Many plant species and cultivars within species, including triticale, are recalcitrant in that the success rate of achieving whole newly generated (diploid) plants is very low. Genotype by culture medium interaction is responsible for varying success rates, as is a high degree of microspore abortion during culturing.(Johansson et al. 2000)[16][17] The response of parental triticale lines to anther culture is known to be correlated to the response of their progeny.[15][18][19] Chromosome elimination is another method of producing DHs, and involves hybridisation of wheat with maize (Zea mays L.), followed by auxin treatment and the artificial rescue of the resultant haploid embryos before they naturally abort. This technique is applied rather extensively to wheat.[20] Its success is in large part due to the insensitivity of maize pollen to the crossability inhibitor genes known as Kr1 and Kr2 that are expressed in the floral style of many wheat cultivars.[21] The technique is unfortunately less successful in triticale.[22] However, Imperata cylindrica (a grass) was found to be just as effective as maize with respect to the production of DHs in both wheat and triticale.[23]

Application of molecular markers

An important advantage of biotechnology applied to plant breeding is the speeding up of cultivar release that would otherwise take 8–12 years. It is the process of selection that is actually enhanced, i.e., retaining that which is desirable or promising and ridding that which is not. This carries with it the aim of changing the genetic structure of the plant population. The website is a valuable resource for marker assisted selection (MAS) protocols relating to R-genes in wheat. MAS is a form of indirect selection. The Catalogue of Gene Symbols mentioned earlier is an additional source of molecular and morphological markers. Again, triticale has not been well characterised with respect to molecular markers, although an abundance of rye molecular markers makes it possible to track rye chromosomes and segments thereof within a triticale background.

Yield improvements of up to 20% have been achieved in hybrid triticale cultivars due to heterosis.[24][25][26] This raises the question of what inbred lines should be crossed (to produce hybrids) with each other as parents to maximize yield in their hybrid progeny. This is termed the 'combining ability' of the parental lines. The identification of good combining ability at an early stage in the breeding programme can reduce the costs associated with 'carrying' a large number of plants (literally thousands) through it, and thus forms part of efficient selection. Combining ability is assessed by taking into consideration all available information on descent (genetic relatedness), morphology, qualitative (simply inherited) traits and biochemical and molecular markers. Exceptionally little information exists on the use of molecular markers to predict heterosis in triticale.[27] Molecular markers are generally accepted as better predictors than morphological markers of (agronomic traits) due to their insensitivity to variation in environmental conditions.

A useful molecular marker known as a simple sequence repeat (SSR) is used in breeding with respect to selection. SSRs are segments of a genome composed of tandem repeats of a short sequence of nucleotides, usually two to six base pairs. They are popular tools in genetics and breeding because of their relative abundance compared to other marker types, a high degree of polymorphism (number of variants), and easy assaying by polymerase chain reaction. However, they are expensive to identify and develop. Comparative genome mapping has revealed a high degree of similarity in terms of sequence colinearity between closely related crop species. This allows the exchange of such markers within a group of related species, such as wheat, rye and triticale. One study established a 58% and 39% transferability rate to triticale from wheat and rye, respectively.[28] Transferability refers to the phenomenon where the sequence of DNA nucleotides flanking the SSR locus (position on the chromosome) is sufficiently homologous (similar) between genomes of closely related species. Thus, DNA primers (a generally short sequence of nucleotides are used to direct a copying reaction during PCR) designed for one species can be used to detect SSRs in related species. SSR markers are available in wheat and rye, but very few, if any, are available for triticale.[28]

Genetic transformation

The genetic transformation of crops involves the incorporation of 'foreign' genes or rather, very small DNA fragments compared to introgression discussed earlier. Amongst other uses, transformation is a useful tool to introduce new traits or characteristics into the transformed crop. Two methods are commonly employed: infectious bacterial-mediated (usually Agrobacterium) and biolistics, with the latter being most commonly applied to allopolyploid cereals such as triticale. Agrobacterium-mediated transformation, however, holds several advantages, such as a low level of transgenic DNA rearrangement, a low number of introduced copies of the transforming DNA, stable integration of an a-priori characterized T-DNA fragment (containing the DNA expressing the trait of interest) and an expected higher level of transgene expression. Triticale has, until recently, only been transformed via biolistics, with a 3.3% success rate (Zimny et al. 1995).[29] Little has been documented on Agrobacterium-mediated transformation of wheat; while no data existed with respect to triticale until 2005, the success rate in later work was nevertheless low.[30]

Conclusion

Triticale holds much promise as a commercial crop, as it has the potential to address specific problems within the cereal industry. Research of a high standard is currently being conducted worldwide in places like Stellenbosch University in South Africa.

Conventional plant breeding has helped establish triticale as a valuable crop, especially where conditions are less favourable for wheat cultivation. Triticale being a synthesized grain notwithstanding, many initial limitations, such as an inability to reproduce due to infertility and seed shrivelling, low yield and poor nutritional value, have been largely eliminated.

Tissue culture techniques with respect to wheat and triticale have seen continuous improvements, but the isolation and culturing of individual microspores seems to hold the most promise. Many molecular markers can be applied to marker-assisted gene transfer, but the expression of R-genes in the new genetic background of triticale remains to be investigated.[28] More than 750 wheat microsatellite primer pairs are available in public wheat breeding programmes, and could be exploited in the development of SSRs in triticale.[28] Another type of molecular marker, single nucleotide polymorphism (SNP), is likely to have a significant impact on the future of triticale breeding.

Health concerns

As triticale contains gluten, it is unsuitable for people with gluten-related disorders, such as celiac disease, non-celiac gluten sensitivity and wheat allergy sufferers, among others.[31]

Triticale in fiction

An episode of the popular TV series Star Trek, "The Trouble With Tribbles", revolved around the protection of a grain developed from triticale, which writer David Gerrold called "quadro-triticale" at producer Gene Coon's suggestion, and to which he ascribed four distinct lobes per kernel. A later episode titled "More Tribbles, More Troubles", in the animated series, also written by Gerrold, dealt with "quinto-triticale", an improvement on the original that apparently had five lobes per kernel.

The "The Trouble With Tribbles" episode attributed the development of triticale to Canada. In 1953, the University of Manitoba began the first North American triticale breeding program. Early breeding efforts concentrated on developing a high yielding, drought tolerant human food crop species suitable for marginal wheat producing areas.[32]

In the same episode, the character Chekov describes the fictional "quadro-triticale" as being a "Famous Russian Inwention."

References

  1. ↑ "Food and Agricultural commodities production". FAO Statistics Division. Retrieved 2016-04-05.
  2. 1 2 Mergoum, Mohamed; Gómez-Macpherson, Helena (2004). "Triticale improvement and production" (PDF). FAO. Retrieved 2010-11-25.
  3. ↑ Larter, E. N. "Triticale". Agriculture. The Canadian Encyclopedia. Retrieved 2009-06-19.
  4. ↑ Sell, J.L.; Hodgson, G.C.; Shebeski, L.H. (1962) Triticale as a potential component of chick rations Canadian Journal of Animal Science, Volume 42, Number 2
  5. ↑ Bird, S. H; Rowe, J. B.; Choct, M.; Stachiw, S.; Tyler, P.; Thompson, R. D. (1999) In vitro fermentation of grain and enzymatic digestion of cereal starch Recent Advances in Animal Nutrition, Vol 12, pp. 53–61
  6. ↑ Alberta Agriculture, Food and Development. "Triticale Production Manual". Government of Alberta, Agricultural and Rural Development. Retrieved 2009-06-23.
  7. ↑ A. R. Hede (2001). "A New Approach to Triticale Improvement" (PDF). Research Highlights of the CIMMYT Wheat Program 1999–2000. Mexico, D. F.: Corporate Communications International Maize and Wheat Improvement Center (CIMMYT). pp. 21–26. Retrieved 18 July 2013.
  8. ↑ Gallais, A. (19–24 June 1983). "Use of Indirect Selection in Plant Breeding. In: Hogenboon, N.G.(ed) et al.". Efficiency In Plant Breeding, Proc. 10th Congress Eucarpia. Pudoc, Wageningen, The Netherlands. pp. 45–60.
  9. ↑ Tikhnenko, N. D.; Tsvetkova, N. V.; Voylokov, A. V. (23 August 2002). "The Effect of Parental Genotypes of Rye Lines on the Development of Quantitative Traits in Primary Octoploid Triticale: Plant Height" (PDF). Russian Journal of Genetics (Russia: MAIK Nauka/Interperiodica) 31 (1): 52–56. doi:10.1023/A:1022070810919. ISSN 1022-7954. Retrieved 2009-06-22.
  10. ↑ Chelkowski, Jerzy; Tyrka, Miroslaw (15 October 2003). "Enhancing the resistance of triticale by using genes from wheat and rye". Journal of Applied Genetics (Poznañ, Poland: Journal of Applied Genetics) 45 (3): 283–295. PMID 15306719. Retrieved 2009-06-22.
  11. ↑ Lee, T. G.; Hong, M. J.; Johnson, J. W.; Bland, D. E.; Kim, D. Y.; Seo, Y. W. (2009). "Development and functional assessment of EST-derived 2RL-specific markers for 2BS.2RL translocations". Theoretical and Applied Genetics (Springer) 119 (4): 663–673. doi:10.1007/s00122-009-1077-3.
  12. ↑ Lukaszewski, Adam (1990). "Frequency of 1RS.1AL and 1RS.1BL Translocations in United States Wheats". Crop Science (Madison, Wisconsin, USA: The Crop Science Society of America) 30 (5): 1151–1153. doi:10.2135/cropsci1990.0011183X003000050041x. Retrieved 2009-06-23.
  13. ↑ Bernard, S.; Charmet, G. (15 June 1984). "Diallel analysis of androgenetic plant production in hexaploid Triticale (X. triticosecale, Wittmack)". TAG Theoretical and Applied Genetics (Berlin / Heidelberg: Springer) 69 (1 / November, 1984): 55–61. doi:10.1007/BF00262539. ISSN 0040-5752. Retrieved 2009-06-19.
  14. ↑ González, J. M.; Jouve, N. (2000). "Improvement of Anther Culture Media for Haploid Production in Triticale". Cereal Research Communications (Hungary: Akadémiai Kiadó) 28: 65–72. ISSN 0133-3720. Retrieved 2009-06-22.
  15. 1 2 González, J. M.; Jouve, N.; Hernádez, I. (January 18, 1997). "Analysis of anther culture response in hexaploid triticale". Plant Breeding (Madrid, Spain: University of Alcal de Henares, Dept. of Cell Biology and Genetics) 116 (3): 301–304. doi:10.1111/j.1439-0523.1997.tb01003.x. ISSN 1439-0523. Retrieved 2009-06-22.
  16. ↑ González, J. M.; Jouve, N. (27 April 2004). "Microspore development during in vitro androgenesis in triticale". Biologia Plantarum (Netherlands: Springer) 49 (1 / March, 2005): 23–28. doi:10.1007/s10535-005-3028-4. ISSN 0006-3134. Retrieved 2009-06-22.
  17. ↑ Johansson, N.; Tuvesson, C.; Ljungberg, A.; Karlsson, K. E.; Suijs, L. W.; Josset, J. P. (2 June 2000). "Large-scale production of wheat and triticale double haploids through the use of a single-anther culture method". Plant Breeding (Svalöv, Sweden: Svalöf Weibull AB) 119 (6): 455–459. doi:10.1046/j.1439-0523.2000.00536.x. ISSN 1439-0523.
  18. ↑ Konzak, Calvin; Zhou, Huaping (1 December 1992). "Genetic control of green plant regeneration from anther culture of wheat". Genome (Ottawa, Canada: National Research Council of Canada Research Press) 35 (6): 957–961. doi:10.1139/g92-146. ISSN 1480-3321. Retrieved 2009-06-23.
  19. ↑ Andersen, S. B.; Tuvesson, I. K. D.; Pedersen, S. (9 August 1989). "Nuclear genes affecting albinism in wheat (Triticum aestivum L.) anther culture". TAG Theoretical and Applied Genetics (Berlin / Heidelberg: Springer) 78 (6 / December, 1989): 879–889. doi:10.1007/BF00266675. ISSN 0040-5752. Retrieved 2009-06-19.
  20. ↑ Bennett, M. D.; Laurie, D. A.; O'Donoughue, L. S. (13–15 March 1989). "Wheat x maize and other wide sexual hybrids: their potential for crop improvement and genetic manipulations.". Gene Manipulation in Plant Improvement II: Proceedings of the 19th Stadler Genetics Symposium. New York: Plenum Press. pp. 95–126.
  21. ↑ Bennett, M. D.; Laurie, D. A. (31 August 1986). "The effect of the crossability loci Kr1 and Kr2 on fertilization frequency in hexaploid wheat x maize crosses". TAG Theoretical and Applied Genetics (Berlin / Heidelberg: Springer) 73 (3 / January, 1987): 403–409. doi:10.1007/BF00262508. ISSN 0040-5752. Retrieved 2009-06-19.
  22. ↑ Marcińska, I.; Wodzony, M.; Ponitka, A.; Ślusarkiewicz-Jarzina, A.; Woźna, J. (26 January 1998). "Production of doubled haploids in triticale (×Triticosecale Wittm.) by means of crosses with maize (Zea mays L.) using picloram and dicamba". Plant Breeding (Kraków, Poland: Polish Academy of Sciences, Dept. of Plant Physiology) 117 (3): 211–215. doi:10.1111/j.1439-0523.1998.tb01928.x. ISSN 1439-0523. Retrieved 2009-06-23.
  23. ↑ Chaudhary, H. K.; Pratap, A.; Sethi, G. S. (August 16, 2004). "Relative efficiency of different Gramineae genera for haploid induction in triticale and triticale x wheat hybrids through the chromosome elimination technique". Plant Breeding (Berlin: Blackwell) 124 (2 / April, 2005): 147–153. doi:10.1111/j.1439-0523.2004.01059.x. ISSN 0179-9541. Retrieved 2009-06-19.
  24. ↑ Góral, H.; et al. (1999). "Heterosis and Combining Ability in Spring Triticale (x Triticosecale, Wittm.)". Plant Breeding and Seed Science (Blonie, Poland: Plant Breeding and Acclimatization Institute at Radzikow) 43: 25–34. Retrieved 2009-06-22.
  25. ↑ Becker, H. C.; Oettler, G.; Hoppe G. (March 8, 2001). "Heterosis for yield and other agronomic traits of winter triticale F1 and F2 hybrids". Plant Breeding (Berlin: Blackwell) 120 (4): 351–353. doi:10.1111/j.1439-0523.2001.tb02016.x. ISSN 0179-9541. Retrieved 2009-06-19.
  26. ↑ Burger, H.; Oettler, G.; Melchinger, A. E. (August 2003). "Heterosis and combining ability for grain yield and other agronomic traits in winter triticale". Plant Breeding (Berlin: Blackwell) 122 (4): 318–321. doi:10.1046/j.1439-0523.2003.00877.x. ISSN 0179-9541. Retrieved 2009-06-19.
  27. ↑ Góral, Halina; Tyrka, Miroslaw; Spiss, Ludwik (2 March 2005). "Assessing genetic variation to predict the breeding value of winter triticale cultivars and lines". Journal of Applied Genetics (Poznañ, Poland: Journal of Applied Genetics) 46 (2): 125–131. PMID 15876679. Retrieved 2009-06-22.
  28. 1 2 3 4 Baenziger, P. S.; Kuleung, C.; Dweikat I. (5 December 2003). "Transferability of SSR markers among wheat, rye, and triticale". TAG Theoretical and Applied Genetics (Berlin / Heidelberg: Springer) 108 (6 / April, 2004): 1147–1150. doi:10.1007/s00122-003-1532-5. ISSN 0040-5752. PMID 15067402. Retrieved 2009-06-19.
  29. ↑ Zinny, J.; Becker, D.; Brettschneider, R.; Lörz, H. (10 October 1994). "Fertile, transgenicTriticale ( ×Triticosecale Wittmack)". Molecular Breeding (Netherlands: Springer) 1 (2): 155–164. doi:10.1007/BF01249700. ISSN 1380-3743. Retrieved 2009-06-23.
  30. ↑ Binka, A.; Nadolska-Orczyk, A.; Przetakiewicz, A.; Kopera, K.; Orczyk, W. (28 July 2005). "Efficient Method of Agrobacterium-mediated Transformation for Triticale (x Triticosecale Wittmack)". Journal of Plant Growth Regulation (New York: Springer) 24 (1 / March, 2005): 2–10. doi:10.1007/s00344-004-0046-y. ISSN 0721-7595. Retrieved 2009-06-19.
  31. ↑ Tovoli F, Masi C, Guidetti E, Negrini G, Paterini P, Bolondi L (Mar 16, 2015). "Clinical and diagnostic aspects of gluten related disorders". World J Clin Cases 3 (3): 275–84. doi:10.12998/wjcc.v3.i3.275. PMC 4360499. PMID 25789300.
  32. ↑ Government of Alberta, Alberta Agriculture and Rural Development, Industry Development and Food Safety, Agriculture Research, Feed Crops. "Triticale". Government of Alberta, Agriculture and Rural Development. Retrieved 11 September 2011.

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