(+)-car-3-ene synthase

(+)-car-3-ene synthase (EC 4.2.3.107, 3-carene cyclase, 3-carene synthase, 3CAR, (+)-3-carene synthase) is an enzyme with systematic name geranyl-diphosphate diphosphate-lyase (cyclizing, (+)-car-3-ene-forming).^[1]^[2]^[3]^[4]^[5]^[6] This enzyme catalyses the following chemical reaction

geranyl diphosphate

\rightleftharpoons

(+)-car-3-ene + diphosphate

The enzyme reacts with (3S)-linalyl diphosphate twice as rapidly as geranyl diphosphate.

References

↑ Savage, T.J. and Croteau, R. (1993). "Biosynthesis of monoterpenes: regio- and stereochemistry of (+)-3-carene biosynthesis". Arch. Biochem. Biophys. 305: 581–587. doi:10.1006/abbi.1993.1464. PMID 8373196.
↑ Savage, T.J., Ichii, H., Hume, S.D., Little, D.B. and Croteau, R. (1995). "Monoterpene synthases from gymnosperms and angiosperms: stereospecificity and inactivation by cysteinyl- and arginyl-directed modifying reagents". Arch. Biochem. Biophys. 320: 257–265. doi:10.1016/0003-9861(95)90008-x. PMID 7625832.
↑ Savage, T.J., Hatch, M.W. and Croteau, R. (1994). "Monoterpene synthases of Pinus contorta and related conifers. A new class of terpenoid cyclase". J. Biol. Chem. 269: 4012–4020. PMID 8307957.
↑ Faldt, J., Martin, D., Miller, B., Rawat, S. and Bohlmann, J. (2003). "Traumatic resin defense in Norway spruce (Picea abies): methyl jasmonate-induced terpene synthase gene expression, and cDNA cloning and functional characterization of (+)-3-carene synthase". Plant Mol. Biol. 51: 119–133. doi:10.1023/A:1020714403780. PMID 12602896.
↑ Hamberger, B., Hall, D., Yuen, M., Oddy, C., Hamberger, B., Keeling, C.I., Ritland, C., Ritland, K. and Bohlmann, J. (2009). "Targeted isolation, sequence assembly and characterization of two white spruce (Picea glauca) BAC clones for terpenoid synthase and cytochrome P₄₅₀ genes involved in conifer defence reveal insights into a conifer genome". BMC Plant Biol. 9: #106–106. doi:10.1186/1471-2229-9-106. PMID 19656416.
↑ Hall, D.E., Robert, J.A., Keeling, C.I., Domanski, D., Quesada, A.L., Jancsik, S., Kuzyk, M.A., Hamberger, B., Borchers, C.H. and Bohlmann, J. (2011). "An integrated genomic, proteomic and biochemical analysis of (+)-3-carene biosynthesis in Sitka spruce (Picea sitchensis) genotypes that are resistant or susceptible to white pine weevil". Plant J. 65: 936–948. doi:10.1111/j.1365-313x.2010.04478.x. PMID 21323772.

External links

( )-car-3-ene synthase at the US National Library of Medicine Medical Subject Headings (MeSH)

Carbon-oxygen lyases (EC 4.2) (primarily dehydratases)

4.2.1: Hydro-Lyases	Carbonic anhydrase Fumarase Aconitase Enolase Alpha Enolase 2 Enoyl-CoA hydratase/3-Hydroxyacyl ACP dehydrase Methylglutaconyl-CoA hydratase Tryptophan synthase Cystathionine beta synthase Porphobilinogen synthase 3-Isopropylmalate dehydratase Urocanase Uroporphyrinogen III synthase Nitrile hydratase

4.2.2: Acting on polysaccharides	Hyaluronate lyase

4.2.3: Acting on phosphates	Threonine synthase

4.2.99: Other	Carboxymethyloxysuccinate lyase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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