(-)-germacrene D synthase

(-)-germacrene D synthase (EC 4.2.3.75) is an enzyme with systematic name (2E,6E)-farnesyl-diphosphate diphosphate-lyase ((-)-germacrene-D-forming).^[1]^[2]^[3]^[4] This enzyme catalyses the following chemical reaction

(2E,6E)-farnesyl diphosphate

\rightleftharpoons

(-)-germacrene D + diphosphate

In Solidago canadensis the biosynthesis results in the pro-R hydrogen at C-1 of the farnesy diphosphate.

References

↑ Schmidt, C.O., Bouwmeester, H.J., Franke, S. and König, W.A. (1999). "Mechanisms of the biosynthesis of sesquiterpene enantiomers (+)- and (-)-germacrene D in Solidago canadensis". Chirality 11: 353–362. doi:10.1002/(sici)1520-636x(1999)11:5/6<353::aid-chir2>3.0.co;2-l.
↑ He, X. and Cane, D.E. (2004). "Mechanism and stereochemistry of the germacradienol/germacrene D synthase of Streptomyces coelicolor A3(2)". J. Am. Chem. Soc. 126: 2678–2679. doi:10.1021/ja039929k. PMID 14995166.
↑ Lucker, J., Bowen, P. and Bohlmann, J. (2004). "Vitis vinifera terpenoid cyclases: functional identification of two sesquiterpene synthase cDNAs encoding (+)-valencene synthase and (-)-germacrene D synthase and expression of mono- and sesquiterpene synthases in grapevine flowers and berries". Phytochemistry 65 (19): 2649–2659. doi:10.1016/j.phytochem.2004.08.017. PMID 15464152.
↑ Prosser, I., Altug, I.G., Phillips, A.L., Konig, W.A., Bouwmeester, H.J. and Beale, M.H. (2004). "Enantiospecific (+)- and (-)-germacrene D synthases, cloned from goldenrod, reveal a functionally active variant of the universal isoprenoid-biosynthesis aspartate-rich motif". Arch. Biochem. Biophys. 432 (2): 136–144. doi:10.1016/j.abb.2004.06.030. PMID 15542052.

External links

(-)-germacrene D synthase at the US National Library of Medicine Medical Subject Headings (MeSH)

Carbon-oxygen lyases (EC 4.2) (primarily dehydratases)

4.2.1: Hydro-Lyases	Carbonic anhydrase Fumarase Aconitase Enolase Alpha Enolase 2 Enoyl-CoA hydratase/3-Hydroxyacyl ACP dehydrase Methylglutaconyl-CoA hydratase Tryptophan synthase Cystathionine beta synthase Porphobilinogen synthase 3-Isopropylmalate dehydratase Urocanase Uroporphyrinogen III synthase Nitrile hydratase

4.2.2: Acting on polysaccharides	Hyaluronate lyase

4.2.3: Acting on phosphates	Threonine synthase

4.2.99: Other	Carboxymethyloxysuccinate lyase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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