2-deoxy-scyllo-inosose synthase

2-deoxy-scyllo-inosose synthase
Identifiers
EC number	4.2.3.124
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

2-deoxy-scyllo-inosose synthase (EC 4.2.3.124, btrC (gene), neoC (gene), kanC (gene)) is an enzyme with systematic name D-glucose-6-phosphate phosphate-lyase (2-deoxy-scyllo-inosose-forming).^{[1][2][3][4][5][6][7]} This enzyme catalyses the following chemical reaction

D-glucose 6-phosphate $\rightleftharpoons$ 2-deoxy-L-scyllo-inosose + phosphate

This enzyme requires Co²⁺.

References

1. Kudo, F., Yamauchi, N., Suzuki, R. and Kakinuma, K. (1997). "Kinetic isotope effect and reaction mechanism of 2-deoxy-scyllo-inosose synthase derived from butirosin-producing Bacillus circulans". J. Antibiot. (Tokyo) 50: 424–428. doi:10.7164/antibiotics.50.424. PMID 9207913.
2. Kudo, F., Hosomi, Y., Tamegai, H. and Kakinuma, K. (1999). "Purification and characterization of 2-deoxy-scyllo-inosose synthase derived from Bacillus circulans. A crucial carbocyclization enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics". J. Antibiot. (Tokyo) 52: 81–88. doi:10.7164/antibiotics.52.81. PMID 10344560.
3. Kudo, F., Tamegai, H., Fujiwara, T., Tagami, U., Hirayama, K. and Kakinuma, K. (1999). "Molecular cloning of the gene for the key carbocycle-forming enzyme in the biosynthesis of 2-deoxystreptamine-containing aminocyclitol antibiotics and its comparison with dehydroquinate synthase". J. Antibiot. (Tokyo) 52: 559–571. doi:10.7164/antibiotics.52.559. PMID 10470681.
4. Huang, Z., Kakinuma, K. and Eguchi, T. (2005). "Stereospecificity of hydride transfer in NAD⁺-catalyzed 2-deoxy-scyllo-inosose synthase, the key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminocyclitol antibiotics". Bioorg. Chem. 33: 82–89. doi:10.1016/j.bioorg.2004.09.002. PMID 15788164.
5. Thuy, M.L., Kharel, M.K., Lamichhane, R., Lee, H.C., Suh, J.W., Liou, K. and Sohng, J.K. (2005). "Expression of 2-deoxy-scyllo-inosose synthase (kanA) from kanamycin gene cluster in Streptomyces lividans". Biotechnol. Lett. 27: 465–470. doi:10.1007/s10529-005-2222-y. PMID 15928851.
6. Tamegai, H., Nango, E., Koike-Takeshita, A., Kudo, F. and Kakinuma, K. (2002). "Significance of the 20-kDa subunit of heterodimeric 2-deoxy-scyllo-inosose synthase for the biosynthesis of butirosin antibiotics in Bacillus circulans". Biosci. Biotechnol. Biochem. 66: 1538–1545. doi:10.1271/bbb.66.1538. PMID 12224638.
7. Tamegai, H., Sawada, H., Nango, E., Aoki, R., Hirakawa, H., Iino, T. and Eguchi, T. (2010). "Roles of a 20 kDa protein associated with a carbocycle-forming enzyme involved in aminoglycoside biosynthesis in primary and secondary metabolism". Biosci. Biotechnol. Biochem. 74: 1215–1219. doi:10.1271/bbb.90961. PMID 20530911.

External links

• 2-deoxy-scyllo-inosose synthase at the US National Library of Medicine Medical Subject Headings (MeSH)