ALG1

ALG1, chitobiosyldiphosphodolichol beta-mannosyltransferase
Identifiers
Symbols	ALG1 ; CDG1K; HMAT1; HMT-1; HMT1; MT-1; Mat-1; hMat-1
External IDs	OMIM: 605907 MGI: 2384774 HomoloGene: 5387 GeneCards: ALG1 Gene
EC number	2.4.1.142
Gene ontology Molecular function • mannosyltransferase activity • chitobiosyldiphosphodolichol beta-mannosyltransferase activity Cellular component • endoplasmic reticulum • endoplasmic reticulum membrane • membrane • integral component of membrane Biological process • protein glycosylation • dolichol-linked oligosaccharide biosynthetic process • lipopolysaccharide biosynthetic process • protein N-linked glycosylation via asparagine • post-translational protein modification • cellular protein metabolic process • mannosylation Sources: Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	56052	208211
Ensembl	ENSG00000033011	ENSMUSG00000039427
UniProt	Q9BT22	Q921Q3
RefSeq (mRNA)	NM_019109	NM_145362
RefSeq (protein)	NP_061982	NP_663337
Location (UCSC)	Chr 16: 5.03 – 5.09 Mb	Chr 16: 5.23 – 5.24 Mb
PubMed search

Chitobiosyldiphosphodolichol beta-mannosyltransferase is an enzyme that is encoded by the ALG1 gene ^[1] whose structure and function has been conserved from yeast to man.^[2][3]

Function

The biosynthesis of lipid-linked oligosaccharides is highly conserved among eukaryotes and is catalyzed by 14 glycosyltransferases in an ordered stepwise manner. The Alg1 mannosyltransferase I (MT I) catalyzes the first mannosylation step in this process.^[2]

References

1. Couto JR, Huffaker TC, Robbins PW (1984). "Cloning and expression in Escherichia coli of a yeast mannosyltransferase from the asparagine-linked glycosylation pathway". J.Biol. Chem 259 (1): 378–82. PMID 6368538.
2. "Entrez Gene: ALG1 asparagine-linked glycosylation 1 homolog (S. cerevisiae, beta-1,4-mannosyltransferase)".
3. Takahashi T, Honda R, Nishikawa Y (Mar 2000). "Cloning of the human cDNA which can complement the defect of the yeast mannosyltransferase I-deficient mutant alg 1". Glycobiology 10 (3): 321–7. doi:10.1093/glycob/10.3.321. PMID 10704531.

Further reading

• Couto JR, Huffaker TC, Robbins PW (January 1984). "Cloning and expression in Escherichia coli of a yeast mannosyltransferase from the asparagine-linked glycosylation pathway". J. Biol. Chem. 259 (1): 378–82. PMID 6368538.
• Gao XD, Nishikawa A, Dean N (June 2004). "Physical interactions between the Alg1, Alg2, and Alg11 mannosyltransferases of the endoplasmic reticulum". Glycobiology 14 (6): 559–70. doi:10.1093/glycob/cwh072. PMID 15044395.
• Otsuki T, Ota T, Nishikawa T; et al. (2007). "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries.". DNA Res. 12 (2): 117–26. doi:10.1093/dnares/12.2.117. PMID 16303743.
• Gerhard DS, Wagner L, Feingold EA; et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Kranz C, Denecke J, Lehle L; et al. (2004). "Congenital Disorder of Glycosylation Type Ik (CDG-Ik): A Defect of Mannosyltransferase I". Am. J. Hum. Genet. 74 (3): 545–51. doi:10.1086/382493. PMC 1182267. PMID 14973782.
• Schwarz M, Thiel C, Lübbehusen J; et al. (2004). "Deficiency of GDP-Man:GlcNAc2-PP-Dolichol Mannosyltransferase Causes Congenital Disorder of Glycosylation Type Ik". Am. J. Hum. Genet. 74 (3): 472–81. doi:10.1086/382492. PMC 1182261. PMID 14973778.
• Grubenmann CE, Frank CG, Hülsmeier AJ; et al. (2004). "Deficiency of the first mannosylation step in the N-glycosylation pathway causes congenital disorder of glycosylation type Ik". Hum. Mol. Genet. 13 (5): 535–42. doi:10.1093/hmg/ddh050. PMID 14709599.
• Clark HF, Gurney AL, Abaya E; et al. (2003). "The Secreted Protein Discovery Initiative (SPDI), a Large-Scale Effort to Identify Novel Human Secreted and Transmembrane Proteins: A Bioinformatics Assessment". Genome Res. 13 (10): 2265–70. doi:10.1101/gr.1293003. PMC 403697. PMID 12975309.
• Strausberg RL, Feingold EA, Grouse LH; et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.

External links

• GeneReviews/NCBI/NIH/UW entry on Congenital Disorders of Glycosylation Overview