Bleomycin hydrolase

Bleomycin hydrolase
PDB rendering based on 1cb5.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1CB5​, 2CB5​
Identifiers
Symbols	BLMH ; BH; BMH
External IDs	OMIM: 602403 MGI: 1345186 HomoloGene: 330 GeneCards: BLMH Gene
EC number	3.4.22.40
Gene ontology Molecular function • aminopeptidase activity • carboxypeptidase activity • cysteine-type endopeptidase activity • protein binding • cysteine-type peptidase activity Cellular component • nucleus • nucleoplasm • cytoplasm • cytosol • extracellular exosome Biological process • protein polyubiquitination • antigen processing and presentation of peptide antigen via MHC class I • proteolysis • response to toxic substance • response to drug Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	642	104184
Ensembl	ENSG00000108578	ENSMUSG00000020840
UniProt	Q13867	Q8R016
RefSeq (mRNA)	NM_000386	NM_178645
RefSeq (protein)	NP_000377	NP_848760
Location (UCSC)	Chr 17: 30.25 – 30.29 Mb	Chr 11: 76.92 – 76.99 Mb
PubMed search

Bleomycin hydrolase is an enzyme that in humans is encoded by the BLMH gene.^[1][2][3]

Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution. Its biological function is hydrolysis of the reactive electrophile homocysteine thiolactone.^[4] Another of its activities is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily.^[3]

Interactions

BLMH has been shown to interact with RPL29,^[5] RPL11,^[5] UBE2I^[6] and Amyloid precursor protein.^[7]

References

1. Ferrando AA, Pendas AM, Llano E, Velasco G, Lidereau R, Lopez-Otin C (January 1998). "Gene characterization, promoter analysis, and chromosomal localization of human bleomycin hydrolase". J Biol Chem 272 (52): 33298–304. doi:10.1074/jbc.272.52.33298. PMID 9407121.
2. Montoya SE, Ferrell RE, Lazo JS (October 1997). "Genomic structure and genetic mapping of the human neutral cysteine protease bleomycin hydrolase". Cancer Res 57 (19): 4191–5. PMID 9331073.
3. "Entrez Gene: BLMH bleomycin hydrolase".
4. Jarosław Zimny, Marta Sikora, Andrzej Guranowski and Hieronim Jakubowski (2006). "Protective Mechanisms against Homocysteine Toxicity". The Journal of Biological Chemistry 281: 22485–22492. doi:10.1074/jbc.M603656200.
5. Koldamova, R P; Lefterov I M; DiSabella M T; Almonte C; Watkins S C; Lazo J S (June 1999). "Human bleomycin hydrolase binds ribosomal proteins". Biochemistry (UNITED STATES) 38 (22): 7111–7. doi:10.1021/bi990135l. ISSN 0006-2960. PMID 10353821.
6. Koldamova, R P; Lefterov I M; DiSabella M T; Lazo J S (December 1998). "An evolutionarily conserved cysteine protease, human bleomycin hydrolase, binds to the human homologue of ubiquitin-conjugating enzyme 9". Mol. Pharmacol. (UNITED STATES) 54 (6): 954–61. ISSN 0026-895X. PMID 9855622.
7. Lefterov, I M; Koldamova R P; Lazo J S (September 2000). "Human bleomycin hydrolase regulates the secretion of amyloid precursor protein". FASEB J. (UNITED STATES) 14 (12): 1837–47. doi:10.1096/fj.99-0938com. ISSN 0892-6638. PMID 10973933.

Further reading

• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1-2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Ferrando AA, Velasco G, Campo E, Lopez-Otin C (1996). "Cloning and expression analysis of human bleomycin hydrolase, a cysteine proteinase involved in chemotherapy resistance.". Cancer Res. 56 (8): 1746–50. PMID 8620487.
• Brömme D, Rossi AB, Smeekens SP; et al. (1996). "Human bleomycin hydrolase: molecular cloning, sequencing, functional expression, and enzymatic characterization.". Biochemistry 35 (21): 6706–14. doi:10.1021/bi960092y. PMID 8639621.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K; et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library.". Gene 200 (1-2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Koldamova RP, Lefterov IM, Gadjeva VG, Lazo JS (1998). "Essential binding and functional domains of human bleomycin hydrolase.". Biochemistry 37 (8): 2282–90. doi:10.1021/bi9722204. PMID 9485374.
• Montoya SE, Aston CE, DeKosky ST; et al. (1998). "Bleomycin hydrolase is associated with risk of sporadic Alzheimer's disease.". Nat. Genet. 18 (3): 211–2. doi:10.1038/ng0398-211. PMID 9500538.
• Zheng W, Johnston SA, Joshua-Tor L (1998). "The unusual active site of Gal6/bleomycin hydrolase can act as a carboxypeptidase, aminopeptidase, and peptide ligase.". Cell 93 (1): 103–9. doi:10.1016/S0092-8674(00)81150-2. PMID 9546396.
• Koldamova RP, Lefterov IM, DiSabella MT, Lazo JS (1999). "An evolutionarily conserved cysteine protease, human bleomycin hydrolase, binds to the human homologue of ubiquitin-conjugating enzyme 9.". Mol. Pharmacol. 54 (6): 954–61. PMID 9855622.
• Takeda A, Nonaka M, Ishikawa A, Higuchi D (1999). "Immunohistochemical localization of the neutral cysteine protease bleomycin hydrolase in human skin.". Arch. Dermatol. Res. 291 (4): 238–40. doi:10.1007/s004030050400. PMID 10335922.
• Koldamova RP, Lefterov IM, DiSabella MT; et al. (1999). "Human bleomycin hydrolase binds ribosomal proteins.". Biochemistry 38 (22): 7111–7. doi:10.1021/bi990135l. PMID 10353821.
• O'Farrell PA, Gonzalez F, Zheng W; et al. (1999). "Crystal structure of human bleomycin hydrolase, a self-compartmentalizing cysteine protease.". Structure 7 (6): 619–27. doi:10.1016/S0969-2126(99)80083-5. PMID 10404591.
• Malherbe P, Faull RL, Richards JG (2000). "Regional and cellular distribution of bleomycin hydrolase mRNA in human brain: comparison between Alzheimer's diseased and control brains.". Neurosci. Lett. 281 (1): 37–40. doi:10.1016/S0304-3940(00)00802-8. PMID 10686410.
• Lefterov IM, Koldamova RP, Lazo JS (2000). "Human bleomycin hydrolase regulates the secretion of amyloid precursor protein.". FASEB J. 14 (12): 1837–47. doi:10.1096/fj.99-0938com. PMID 10973933.
• Strausberg RL, Feingold EA, Grouse LH; et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Nivet-Antoine V, Coulhon MP, Le Denmat C; et al. (2003). "[Apolipoprotein E and bleomycin hydrolase. Polymorphisms: association with neurodegenerative diseases]". Ann. Biol. Clin. (Paris) 61 (1): 61–7. PMID 12604387.
• Gerhard DS, Wagner L, Feingold EA; et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Rual JF, Venkatesan K, Hao T; et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network.". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.