Clostripain

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Clostripain (EC 3.4.22.8, clostridiopeptidase B, clostridium histolyticum proteinase B, alpha-clostridipain, clostridiopeptidase, Endoproteinase Arg-C) is a proteinase that cleaves proteins on the carboxyl peptide bond of arginine.^[1]^[2] It was isolated from Clostridium histolyticum. The isoelectric point of the enzyme is 4.8-4.9 (at 8°C), and optimum pH is 7.4~7.8 (against α-benzoyl-arginine ethyl ester). The composition of the enzyme is indicated to be of two chains of relative molecular mass 45,000 and 12,500.^[3]

References

↑ Mitchell, W.M. (1977). "Cleavage at arginine residues by clostripain". Methods Enzymol. 47: 165–170. doi:10.1016/0076-6879(77)47020-4. PMID 927173.
↑ Gilles, A.-M., Imhoff, J.-M. and Keil, B. (1979). "α-Clostripain. Chemical characterization, activity, and thiol content of the highly active form of clostripain". J. Biol. Chem. 254: 1462–1468. PMID 762145.
↑ Gilles, A.-M., Lecroisey, A. and Keil, B. (1984). "Primary structure of α-clostripain light chain". Eur. J. Biochem. 145: 469–476. doi:10.1111/j.1432-1033.1984.tb08579.x. PMID 6391922.

External links

clostripain at the US National Library of Medicine Medical Subject Headings (MeSH)
EC 3.4.22.8

Proteases: cysteine proteases (EC 3.4.22)

Caspase	Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase 13 Caspase 14

Fruit-derived	Papain Ficain Bromelain Actinidain

Calpain	CAPN1 CAPN2 CAPN3 CAPN5 CAPN6 CAPN7 CAPN8 CAPN9 CAPN10 CAPN11 CAPN12 CAPN13 CAPN14 CAPNS1 CAPNS2

Cathepsin	B C F H K L1/L2 O S W Z

Other	Clostripain Cancer procoagulant Separase Autophagin Cruzipain

Proteins: enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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