Levan fructotransferase (DFA-IV-forming)

Produces di-beta-D-fructofuranose 2,6':2',6-dianhydride (DFA IV) by successively eliminating the diminishing (2->6)-beta-D-fructan (levan) chain from the terminal D-fructosyl-D-fructosyl disaccharide

This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on polysaccharides. The systematic name of this enzyme class is 2,6-beta-D-fructan lyase (di-beta-D-fructofuranose-2,6':2',6-dianhydride-forming). Other names in common use include 2,6-beta-D-fructan D-fructosyl-D-fructosyltransferase (forming, di-beta-D-fructofuranose 2,6':2',6-dianhydride), and levan fructotransferase.

References

Song KB, Bae KS, Lee YB, Lee KY and Rhee SK (2000). "Characteristics of levan fructotransferase from Arthrobacter ureafaciens K2032 and difructose anhydride IV formation from levan". Enzyme and Microbial Technology 27 (3–5): 212–218. doi:10.1016/S0141-0229(00)00135-6.
Rhee SK; Ryu, EJ; Park, BS; Song, KB; Kang, SA; Kim, CH; Uhm, TB; Park, YI; Rhee, SK (2003). "Levan fructotransferase from Arthrobacter oxydans J17-21 catalyzes the formation of the di-D-fructose dianhydride IV from levan". J. Agric. Food. Chem. 51 (9): 2632–6. doi:10.1021/jf026207o. PMID 12696949.
Saito K, Tomita F (2000). "Difructose anhydrides: their mass-production and physiological functions". Biosci. Biotechnol. Biochem. 64 (7): 1321–7. doi:10.1271/bbb.64.1321. PMID 10945246.

Carbon-oxygen lyases (EC 4.2) (primarily dehydratases)

4.2.1: Hydro-Lyases	Carbonic anhydrase Fumarase Aconitase Enolase Alpha Enolase 2 Enoyl-CoA hydratase/3-Hydroxyacyl ACP dehydrase Methylglutaconyl-CoA hydratase Tryptophan synthase Cystathionine beta synthase Porphobilinogen synthase 3-Isopropylmalate dehydratase Urocanase Uroporphyrinogen III synthase Nitrile hydratase

4.2.2: Acting on polysaccharides	Hyaluronate lyase

4.2.3: Acting on phosphates	Threonine synthase

4.2.99: Other	Carboxymethyloxysuccinate lyase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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