Methanogen homoaconitase

Methanogen homoaconitase (EC 4.2.1.114, methanogen HACN) is an enzyme with systematic name (R)-2-hydroxybutane-1,2,4-tricarboxylate hydro-lyase ((1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate-forming).^[1] This enzyme catalyses the following chemical reaction

(R)-2-hydroxybutane-1,2,4-tricarboxylate

\rightleftharpoons

(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate (overall reaction)

(1a) (R)-2-hydroxybutane-1,2,4-tricarboxylate

\rightleftharpoons

(Z)-but-1-ene-1,2,4-tricarboxylate + H₂O

(1b) (Z)-but-1-ene-1,2,4-tricarboxylate + H₂O

\rightleftharpoons

(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate

This enzyme catalyses several reactions in the pathway of coenzyme-B biosynthesis in methanogenic archaea.

References

↑ Drevland, R.M., Jia, Y., Palmer, D.R. and Graham, D.E. (2008). "Methanogen homoaconitase catalyzes both hydrolyase reactions in coenzyme B biosynthesis". J. Biol. Chem. 283: 28888–28896. doi:10.1074/jbc.m802159200. PMID 18765671.

External links

Methanogen homoaconitase at the US National Library of Medicine Medical Subject Headings (MeSH)

Carbon-oxygen lyases (EC 4.2) (primarily dehydratases)

4.2.1: Hydro-Lyases	Carbonic anhydrase Fumarase Aconitase Enolase Alpha Enolase 2 Enoyl-CoA hydratase/3-Hydroxyacyl ACP dehydrase Methylglutaconyl-CoA hydratase Tryptophan synthase Cystathionine beta synthase Porphobilinogen synthase 3-Isopropylmalate dehydratase Urocanase Uroporphyrinogen III synthase Nitrile hydratase

4.2.2: Acting on polysaccharides	Hyaluronate lyase

4.2.3: Acting on phosphates	Threonine synthase

4.2.99: Other	Carboxymethyloxysuccinate lyase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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