Nuclear-inclusion-a endopeptidase

Nuclear-inclusion-a endopeptidase
Identifiers
EC number	3.4.22.44
CAS number	139946-51-3
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Nuclear-inclusion-a endopeptidase (EC 3.4.22.44, potyvirus NIa protease) is a protease enzyme found in potyviruses.^[1][2][3][4] This enzyme catalyses the following chemical reaction:

Hydrolyses glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus.

e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln \ (Ser or Gly) for the enzyme from tobacco etch virus.

The enzyme is used encoded in vectors for the artificial expression of recombinant fusion proteins (see TEV protease).

References

1. Fellers, J.P., Collins, G.B. and Hunt, A.G. (1998). "The NIa-proteinase of different plant potyviruses provides specific resistance to viral infection". Crop Sci. 38: 1309–1319. doi:10.2135/cropsci1998.0011183x003800050030x.
2. Kim, D.-H. and Choi, K.Y. (1998). "Potyvirus NIa protease". In Barrett, A.J., Rawlings, N.D. and Woessner, J.F. Handbook of Proteolytic Enzymes. London: Academic Press. pp. 721–723.
3. Takahashi, T., Nakanishi, M., Yao, Y., Uyeda, I. and Serizawa, N. (1998). "Direct formation of human interleukin-11 by cis-acting system of plant virus protease in Escherichia coli". Biosci. Biotechnol. Biochem. 62: 953–958. doi:10.1271/bbb.62.953. PMID 9648226.
4. Kim, D.H., Hwang, D.C., Kang, B.H., Lew, J., Han, J.S., Song, B.O.D. and Choi, K.Y. (1996). "Effects of internal cleavages and mutations in the C-terminal region of NIa protease of turnip mosaic potyvirus on the catalytic activity". Virology 226: 183–190. doi:10.1006/viro.1996.0645. PMID 8955037.

External links

• Nuclear-inclusion-a endopeptidase at the US National Library of Medicine Medical Subject Headings (MeSH)