Patchoulol synthase

Patchoulol synthase (EC 4.2.3.70) is an enzyme with systematic name (2E,6E)-farnesyl-diphosphate diphosphate-lyase (patchoulol-forming).^[1]^[2]^[3] This enzyme catalyses the following chemical reaction

(2E,6E)-farnesyl diphosphate + H₂O

\rightleftharpoons

patchoulol + diphosphate

References

↑ Croteau, R., Munck, S.L., Akoh, C.C., Fisk, H.J. and Satterwhite, D.M. (1987). "Biosynthesis of the sesquiterpene patchoulol from farnesyl pyrophosphate in leaf extracts of Pogostemon cablin (patchouli): mechanistic considerations". Arch. Biochem. Biophys. 256: 56–68. doi:10.1016/0003-9861(87)90425-5. PMID 3038029.
↑ Munck, S.L. and Croteau, R. (1990). "Purification and characterization of the sesquiterpene cyclase patchoulol synthase from Pogostemon cablin". Arch. Biochem. Biophys. 282: 58–64. doi:10.1016/0003-9861(90)90086-e. PMID 2171435.
↑ Faraldos, J.A., Wu, S., Chappell, J. and Coates, R.M. (2010). "Doubly deuterium-labeled patchouli alcohol from cyclization of singly labeled [2-²H₁]farnesyl diphosphate catalyzed by recombinant patchoulol synthase". J. Am. Chem. Soc. 132: 2998–3008. doi:10.1021/ja909251r. PMID 20148554.

External links

Patchoulol synthase at the US National Library of Medicine Medical Subject Headings (MeSH)

Carbon-oxygen lyases (EC 4.2) (primarily dehydratases)

4.2.1: Hydro-Lyases	Carbonic anhydrase Fumarase Aconitase Enolase Alpha Enolase 2 Enoyl-CoA hydratase/3-Hydroxyacyl ACP dehydrase Methylglutaconyl-CoA hydratase Tryptophan synthase Cystathionine beta synthase Porphobilinogen synthase 3-Isopropylmalate dehydratase Urocanase Uroporphyrinogen III synthase Nitrile hydratase

4.2.2: Acting on polysaccharides	Hyaluronate lyase

4.2.3: Acting on phosphates	Threonine synthase

4.2.99: Other	Carboxymethyloxysuccinate lyase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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