Poly(alpha-L-guluronate) lyase

Eliminative cleavage of polysaccharides containing a terminal alpha-L-guluronate group, to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enuronosyl groups at their non-reducing ends

This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on polysaccharides. The systematic name of this enzyme class is poly(alpha-L-1,4-guluronide) exo-lyase. Other names in common use include alginase II, guluronate lyase, L-guluronan lyase, L-guluronate lyase, poly-alpha-L-guluronate lyase, and polyguluronate-specific alginate lyase.

Structural studies

As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code 1UAI.

References

Boyd J, Turvey JR (1977). "Isolation of poly-alpha-L-guluronate lyase from Klebsiella aerogenes". Carbohydr. Res. 57: 163–71. doi:10.1016/S0008-6215(00)81928-X. PMID 332364.
Davidson IW, Sutherland IW, Lawson CJ (1976). "Purification and properties of an alginate lyase from a marine bacterium". Biochem. J. 159 (3): 707–13. PMC 1164172. PMID 1008828.

Carbon-oxygen lyases (EC 4.2) (primarily dehydratases)

4.2.1: Hydro-Lyases	Carbonic anhydrase Fumarase Aconitase Enolase Alpha Enolase 2 Enoyl-CoA hydratase/3-Hydroxyacyl ACP dehydrase Methylglutaconyl-CoA hydratase Tryptophan synthase Cystathionine beta synthase Porphobilinogen synthase 3-Isopropylmalate dehydratase Urocanase Uroporphyrinogen III synthase Nitrile hydratase

4.2.2: Acting on polysaccharides	Hyaluronate lyase

4.2.3: Acting on phosphates	Threonine synthase

4.2.99: Other	Carboxymethyloxysuccinate lyase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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