Rhamnogalacturonan exolyase

Rhamnogalacturonan exolyase (EC 4.2.2.24, YesX) is an enzyme with systematic name alpha-L-rhamnopyranosyl-(1->4)-alpha-D-galactopyranosyluronate exolyase.^[1]^[2] This enzyme catalyses the following chemical reaction

Exotype eliminative cleavage of alpha-L-rhamnopyranosyl-(1->4)-alpha-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I oligosacharides containing alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end.

The enzyme is part of the degradation system for rhamnogalacturonan I in Bacillus subtilis strain 168.

References

↑ Ochiai, A., Itoh, T., Mikami, B., Hashimoto, W. and Murata, K. (2009). "Structural determinants responsible for substrate recognition and mode of action in family 11 polysaccharide lyases". J. Biol. Chem. 284: 10181–10189. doi:10.1074/jbc.m807799200. PMID 19193638.
↑ Ochiai, A., Itoh, T., Kawamata, A., Hashimoto, W. and Murata, K. (2007). "Plant cell wall degradation by saprophytic Bacillus subtilis strains: gene clusters responsible for rhamnogalacturonan depolymerization". Appl. Environ. Microbiol. 73: 3803–3813. doi:10.1128/aem.00147-07. PMID 17449691.

External links

Rhamnogalacturonan exolyase at the US National Library of Medicine Medical Subject Headings (MeSH)

Carbon-oxygen lyases (EC 4.2) (primarily dehydratases)

4.2.1: Hydro-Lyases	Carbonic anhydrase Fumarase Aconitase Enolase Alpha Enolase 2 Enoyl-CoA hydratase/3-Hydroxyacyl ACP dehydrase Methylglutaconyl-CoA hydratase Tryptophan synthase Cystathionine beta synthase Porphobilinogen synthase 3-Isopropylmalate dehydratase Urocanase Uroporphyrinogen III synthase Nitrile hydratase

4.2.2: Acting on polysaccharides	Hyaluronate lyase

4.2.3: Acting on phosphates	Threonine synthase

4.2.99: Other	Carboxymethyloxysuccinate lyase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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