Cellobiose phosphorylase

cellobiose phosphorylase
Identifiers
EC number	2.4.1.20
CAS number	9030-20-0
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / EGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, a cellobiose phosphorylase (EC 2.4.1.20) is an enzyme that catalyzes the chemical reaction

cellobiose + phosphate $\rightleftharpoons$ alpha-D-glucose 1-phosphate + D-glucose

Thus, the two substrates of this enzyme are cellobiose and phosphate, whereas its two products are alpha-D-glucose 1-phosphate and D-glucose.

This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is cellobiose:phosphate alpha-D-glucosyltransferase. This enzyme participates in starch and sucrose metabolism.

Structural studies

As of late 2006, two structures have been solved for this class of enzymes, with PDB accession codes 2CQS and 2CQT.

References

• Alexander JK (1968). "Purification and specificity of cellobiose phosphorylase from Clostridium thermocellum". J. Biol. Chem. 243 (11): 2899–904. PMID 5653182.
• AYERS WA (1959). "Phosphorolysis and synthesis of cellobiose by cell extracts from Ruminococcus flavefaciens". J. Biol. Chem. 234: 2819–22. PMID 13795349.