D-Man-alpha-(1-3)-D-Glc-beta-(1-4)-D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronyltransferase

D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronyltransferase
Identifiers
EC number	2.4.1.264
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronyltransferase (EC 2.4.1.264, GumK) is an enzyme with systematic name UDP-glucuronate:D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol beta-1,2-glucuronyltransferase.^{[1][2][3][4][5][6][7]} This enzyme catalyses the following chemical reaction

UDP-glucuronate + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol $\rightleftharpoons$ UDP + D-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol

The enzyme is involved in the biosynthesis of the exopolysaccharides xanthan (in the bacterium Xanthomonas campestris) and acetan (in the bacterium Gluconacetobacter xylinus).

References

1. Katzen, F., Ferreiro, D.U., Oddo, C.G., Ielmini, M.V., Becker, A., Puhler, A. and Ielpi, L. (1998). "Xanthomonas campestris pv. campestris gum mutants: effects on xanthan biosynthesis and plant virulence". J. Bacteriol. 180 (7): 1607–1617. PMC 107069. PMID 9537354.
2. Ielpi, L., Couso, R.O. and Dankert, M.A. (1993). "Sequential assembly and polymerization of the polyprenol-linked pentasaccharide repeating unit of the xanthan polysaccharide in Xanthomonas campestris". J. Bacteriol. 175 (9): 2490–2500. PMC 204549. PMID 7683019.
3. Kim, S.Y., Kim, J.G., Lee, B.M. and Cho, J.Y. (2009). "Mutational analysis of the gum gene cluster required for xanthan biosynthesis in Xanthomonas oryzae pv oryzae". Biotechnol. Lett. 31 (2): 265–270. doi:10.1007/s10529-008-9858-3. PMID 18854951.
4. Barreras, M., Bianchet, M.A. and Ielpi, L. (2006). "Crystallization and preliminary crystallographic characterization of GumK, a membrane-associated glucuronosyltransferase from Xanthomonas campestris required for xanthan polysaccharide synthesis". Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 62 (Pt 9): 880–883. doi:10.1107/S1744309106028764. PMC 2242869. PMID 16946469.
5. Barreras, M., Salinas, S.R., Abdian, P.L., Kampel, M.A. and Ielpi, L. (2008). "Structure and mechanism of GumK, a membrane-associated glucuronosyltransferase". J. Biol. Chem. 283 (36): 25027–25035. doi:10.1074/jbc.M801227200. PMC 3259810. PMID 18596046.
6. Vojnov, A.A., Bassi, D.E., Daniels, M.J. and Dankert, M.A. (2002). "Biosynthesis of a substituted cellulose from a mutant strain of Xanthomonas campestris". Carbohydr. Res. 337 (4): 315–326. doi:10.1016/S0008-6215(01)00322-6. PMID 11841812.
7. Barreras, M., Abdian, P.L. and Ielpi, L. (2004). "Functional characterization of GumK, a membrane-associated β-glucuronosyltransferase from Xanthomonas campestris required for xanthan polysaccharide synthesis". Glycobiology 14 (3): 233–241. doi:10.1093/glycob/cwh056. PMID 14736729.

External links

• [//www.nlm.nih.gov/cgi/mesh/2011/MB_cgi?mode=&term=D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol+2-beta-glucuronyltransferase D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronyltransferase] at the US National Library of Medicine Medical Subject Headings (MeSH)