A-1,3-mannosyl-glycoprotein 2-b-N-acetylglucosaminyltransferase

Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
Identifiers
EC number	2.4.1.101
CAS number	102576-81-8
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (EC 2.4.1.101, N-acetylglucosaminyltransferase I, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I, uridine diphosphoacetylglucosamine-alpha-1,3-mannosylglycoprotein beta-1,2-N-acetylglucosaminyltransferase, UDP-N-acetylglucosaminyl:alpha-1,3-D-mannoside-beta-1,2-N-acetylglucosaminyltransferase I, UDP-N-acetylglucosaminyl:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I, alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase, GnTI) is an enzyme with systematic name UDP-N-acetyl-D-glucosamine:3-(alpha-D-mannosyl)-beta-D-mannosyl-glycoprotein 2-beta-N-acetyl-D-glucosaminyltransferase.^{[1][2][3][4][5][6][7][8]} This enzyme catalyses the following chemical reaction

UDP-N-acetyl-D-glucosamine + 3-(alpha-D-mannosyl)-beta-D-mannosyl-R $\rightleftharpoons$ UDP + 3-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R

R represents the remainder of the N-linked oligosaccharide in the glycoprotein acceptor.

References

1. Harpaz, N. and Schachter, H. (1980). "Control of glycoprotein synthesis. Bovine colostrum UDP-N-acetylglucosamine:α-D-mannoside β2-N-acetylglucosaminyltransferase I. Separation from UDP-N-acetylglucosamine:α-D-mannoside β2-N-acetylglucosaminyltransferase II, partial purification, and substrate specificity". J. Biol. Chem. 255 (10): 4885–4893. PMID 6445358.
2. Mendicino, J., Chandrasekaran, E.V., Anumula, K.R. and Davila, M. (1981). "Isolation and properties of α-D-mannose:β-1,2-N-acetylglucosaminyltransferase from trachea mucosa". Biochemistry 20 (4): 967–976. doi:10.1021/bi00507a050. PMID 6452163.
3. Miyagi, T. and Tsuiki, S. (1981). "Studies on UDP-N-acetylglucosamine : α-mannoside β-N-acetylglucosaminyltransferase of rat liver and hepatomas". Biochim. Biophys. Acta 661 (1): 148–157. doi:10.1016/0005-2744(81)90094-2. PMID 6170335.
4. Oppenheimer, C.L., Eckhardt, A.E. and Hill, R.L. (1981). "The nonidentity of porcine N-acetylglucosaminyltransferases I and II". J. Biol. Chem. 256 (22): 11477–11482. PMID 6457827.
5. Oppenheimer, C.L. and Hill, R.L. (1981). "Purification and characterization of a rabbit liver α1→3 mannoside β1→2 N-acetylglucosaminyltransferase". J. Biol. Chem. 256 (2): 799–804. PMID 6450208.
6. Schachter, H., Narasimhan, S., Gleeson, P. and Vella, G. (1983). "Glycosyltransferases involved in elongation of N-glycosidically linked oligosaccharides of the complex or N-acetyllactosamine type". Methods Enzymol. 98: 98–134. doi:10.1016/0076-6879(83)98143-0. PMID 6366476.
7. Vella, G.J., Paulsen, H. and Schachter, H. (1984). "Control of glycoprotein synthesis. IX. A terminal Man alphal-3Man β1- sequence in the substrate is the minimum requirement for UDP-N-acetyl-D-glucosamine: α-D-mannoside (GlcNAc to Man α1-3) β2-N-acetylglucosaminyltransferase I". Can. J. Biochem. Cell Biol. 62 (6): 409–417. doi:10.1139/o84-056. PMID 6235906.
8. Unligil, U.M., Zhou, S., Yuwaraj, S., Sarkar, M., Schachter, H. and Rini, J.M. (2000). "X-ray crystal structure of rabbit N-acetylglucosaminyltransferase I: catalytic mechanism and a new protein superfamily". EMBO J. 19 (20): 5269–5280. doi:10.1093/emboj/19.20.5269. PMID 11032794.

External links

• Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase at the US National Library of Medicine Medical Subject Headings (MeSH)