Galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase

Galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase
Identifiers
EC number	2.4.1.288
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase (EC 2.4.1.288, GlfT2) is an enzyme with systematic name UDP-alpha-D-galactofuranose:beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol 4-beta/5-beta-D-galactofuranosyltransferase.^[1][2][3] This enzyme catalyses the following chemical reaction

28 UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol $\rightleftharpoons$ 28 UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol

This enzyme is solated from Mycobacterium tuberculosis.

References

1. Rose, N.L., Zheng, R.B., Pearcey, J., Zhou, R., Completo, G.C. and Lowary, T.L. (2008). "Development of a coupled spectrophotometric assay for GlfT2, a bifunctional mycobacterial galactofuranosyltransferase". Carbohydr. Res. 343 (12): 2130–2139. doi:10.1016/j.carres.2008.03.023. PMID 18423586.
2. May, J.F., Splain, R.A., Brotschi, C. and Kiessling, L.L.; Splain; Brotschi; Kiessling (2009). "A tethering mechanism for length control in a processive carbohydrate polymerization". Proc. Natl. Acad. Sci. USA 106 (29): 11851–11856. Bibcode:2009PNAS..10611851M. doi:10.1073/pnas.0901407106. PMC 2715488. PMID 19571009.
3. Wheatley, R.W., Zheng, R.B., Richards, M.R., Lowary, T.L. and Ng, K.K. (2012). "Tetrameric structure of GlfT2 reveals a scaffold for the assembly of mycobacterial arabinogalactan". J. Biol. Chem. 287 (33): 28132–43. doi:10.1074/jbc.M112.347484. PMC 3431634. PMID 22707726.

External links

• Galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase at the US National Library of Medicine Medical Subject Headings (MeSH)