Lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase

Lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase
Identifiers
EC number	2.4.2.43
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase (EC 2.4.2.43, undecaprenyl phosphate-alpha-L-Ara4N transferase, 4-amino-4-deoxy-L-arabinose lipid A transferase, polymyxin resistance protein PmrK, arnT (gene)) is an enzyme with systematic name 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate:lipid IVA 4-amino-4-deoxy-L-arabinopyranosyltransferase.^{[1][2][3][4][5]} This enzyme catalyses the following chemical reaction

(1) 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate + alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid A $\rightleftharpoons$ alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[4-P-L-Ara4N]-lipid A + ditrans,octacis-undecaprenyl phosphate

(2) 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate + lipid IVA $\rightleftharpoons$ lipid IIA + ditrans,octacis-undecaprenyl phosphate

(3) 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate + alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA $\rightleftharpoons$ 4'-alpha-L-Ara4N-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA + ditrans,octacis-undecaprenyl phosphate

This integral membrane protein is present in the inner membrane of certain Gram negative endobacteria.

References

1. Trent, M.S., Ribeiro, A.A., Lin, S., Cotter, R.J. and Raetz, C.R. (2001). "An inner membrane enzyme in Salmonella and Escherichia coli that transfers 4-amino-4-deoxy-L-arabinose to lipid A: induction on polymyxin-resistant mutants and role of a novel lipid-linked donor". J. Biol. Chem. 276: 43122–43131. doi:10.1074/jbc.m106961200. PMID 11535604.
2. Trent, M.S., Ribeiro, A.A., Doerrler, W.T., Lin, S., Cotter, R.J. and Raetz, C.R. (2001). "Accumulation of a polyisoprene-linked amino sugar in polymyxin-resistant Salmonella typhimurium and Escherichia coli: structural characterization and transfer to lipid A in the periplasm". J. Biol. Chem. 276: 43132–43144. doi:10.1074/jbc.m106962200. PMID 11535605.
3. Zhou, Z., Ribeiro, A.A., Lin, S., Cotter, R.J., Miller, S.I. and Raetz, C.R. (2001). "Lipid A modifications in polymyxin-resistant Salmonella typhimurium: PMRA-dependent 4-amino-4-deoxy-L-arabinose, and phosphoethanolamine incorporation". J. Biol. Chem. 276: 43111–43121. doi:10.1074/jbc.m106960200. PMID 11535603.
4. Bretscher, L.E., Morrell, M.T., Funk, A.L. and Klug, C.S. (2006). "Purification and characterization of the L-Ara4N transferase protein ArnT from Salmonella typhimurium". Protein Expr. Purif. 46: 33–39. doi:10.1016/j.pep.2005.08.028. PMID 16226890.
5. Impellitteri, N.A., Merten, J.A., Bretscher, L.E. and Klug, C.S. (2010). "Identification of a functionally important loop in Salmonella typhimurium ArnT". Biochemistry 49: 29–35. doi:10.1021/bi901572f. PMID 19947657.

External links

• Lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase at the US National Library of Medicine Medical Subject Headings (MeSH)