RNASE1

Ribonuclease, RNase A family, 1 (pancreatic)
PDB rendering based on 1dza.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1DZA​, 1E21​, 1H8X​, 1Z7X​, 2E0J​, 2E0L​, 2E0M​, 2E0O​, 2K11​, 2Q4G​, 3F8G​, 4KXH​
Identifiers
Symbols	RNASE1 ; RAC1; RIB1; RNS1
External IDs	OMIM: 180440 MGI: 97919 HomoloGene: 7919 ChEMBL: 5425 GeneCards: RNASE1 Gene
EC number	3.1.27.5
Gene ontology Molecular function • nucleic acid binding • ribonuclease A activity • protein binding Cellular component • extracellular exosome Biological process • RNA phosphodiester bond hydrolysis, endonucleolytic Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	6035	19752
Ensembl	ENSG00000129538	ENSMUSG00000035896
UniProt	P07998	P00683
RefSeq (mRNA)	NM_002933	NM_011271
RefSeq (protein)	NP_002924	NP_035401
Location (UCSC)	Chr 14: 20.8 – 20.8 Mb	Chr 14: 51.15 – 51.15 Mb
PubMed search

Ribonuclease pancreatic is an enzyme that in humans is encoded by the RNASE1 gene.^[1][2]

Function

This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. The encoded protein is monomeric and more commonly acts to degrade ss-RNA over ds-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.^[2]

References

1. Kochetov AV, Lukasheva VV, Filipenko ML, Mertvetsov NP, Rivkin MI (Sep 1995). "[Primary structure of the coding part of the gene for human pancreatic ribonuclease and its chromosomal location]". Bioorganicheskaia Khimiia 21 (9): 691–4. PMID 8588814.
2. "Entrez Gene: RNASE1 ribonuclease, RNase A family, 1 (pancreatic)".

Further reading

• Nogués MV, Vilanova M, Cuchillo CM (Nov 1995). "Bovine pancreatic ribonuclease A as a model of an enzyme with multiple substrate binding sites". Biochimica et Biophysica Acta 1253 (1): 16–24. doi:10.1016/0167-4838(95)00138-k. PMID 7492594.
• Schienman JE, Holt RA, Auerbach MR, Stewart CB (Aug 2006). "Duplication and divergence of 2 distinct pancreatic ribonuclease genes in leaf-eating African and Asian colobine monkeys". Molecular Biology and Evolution 23 (8): 1465–79. doi:10.1093/molbev/msl025. PMID 16751256.
• Sakakibara R, Hashida K, Kitahara T, Ishiguro M (Mar 1992). "Characterization of a unique nonsecretory ribonuclease from urine of pregnant women". Journal of Biochemistry 111 (3): 325–30. PMID 1587793.
• Haugg M, Schein CH (Feb 1992). "The DNA sequences of the human and hamster secretory ribonucleases determined with the polymerase chain reaction (PCR)". Nucleic Acids Research 20 (3): 612. doi:10.1093/nar/20.3.612. PMC 310435. PMID 1741299.
• Sakakibara R, Hashida K, Tominaga N, Sakai K, Ishiguro M, Imamura S, Ohmatsu F, Sato E (Jan 1991). "A putative mouse oocyte maturation inhibitory protein from urine of pregnant women: N-terminal sequence homology with human nonsecretory ribonuclease". Chemical & Pharmaceutical Bulletin 39 (1): 146–9. doi:10.1248/cpb.39.146. PMID 2049798.
• Mizuta K, Awazu S, Yasuda T, Kishi K (Aug 1990). "Purification and characterization of three ribonucleases from human kidney: comparison with urine ribonucleases". Archives of Biochemistry and Biophysics 281 (1): 144–51. doi:10.1016/0003-9861(90)90424-W. PMID 2383019.
• Beintema JJ, Blank A, Schieven GL, Dekker CA, Sorrentino S, Libonati M (Oct 1988). "Differences in glycosylation pattern of human secretory ribonucleases". The Biochemical Journal 255 (2): 501–5. PMC 1135256. PMID 3202829.
• Beintema JJ, Wietzes P, Weickmann JL, Glitz DG (Jan 1984). "The amino acid sequence of human pancreatic ribonuclease". Analytical Biochemistry 136 (1): 48–64. doi:10.1016/0003-2697(84)90306-3. PMID 6201087.
• Russo N, de Nigris M, Ciardiello A, Di Donato A, D'Alessio G (Aug 1995). "Expression in mammalian cells, purification and characterization of recombinant human pancreatic ribonuclease". FEBS Letters 369 (2-3): 352. doi:10.1016/0014-5793(95)96890-G. PMID 7649283.
• Seno M, Futami J, Kosaka M, Seno S, Yamada H (Aug 1994). "Nucleotide sequence encoding human pancreatic ribonuclease". Biochimica et Biophysica Acta 1218 (3): 466–8. doi:10.1016/0167-4781(94)90208-9. PMID 8049276.
• Yasuda T, Nadano D, Takeshita H, Kishi K (Dec 1993). "Two distinct secretory ribonucleases from human cerebrum: purification, characterization and relationships to other ribonucleases". The Biochemical Journal 296 (3): 617–25. doi:10.1042/bj2960617. PMC 1137742. PMID 8280059.
• Papageorgiou AC, Shapiro R, Acharya KR (Sep 1997). "Molecular recognition of human angiogenin by placental ribonuclease inhibitor--an X-ray crystallographic study at 2.0 A resolution". The EMBO Journal 16 (17): 5162–77. doi:10.1093/emboj/16.17.5162. PMC 1170149. PMID 9311977.
• Klink TA, Woycechowsky KJ, Taylor KM, Raines RT (Jan 2000). "Contribution of disulfide bonds to the conformational stability and catalytic activity of ribonuclease A". European Journal of Biochemistry / FEBS 267 (2): 566–72. doi:10.1046/j.1432-1327.2000.01037.x. PMID 10632727.
• Pous J, Canals A, Terzyan SS, Guasch A, Benito A, Ribó M, Vilanova M, Coll M (Oct 2000). "Three-dimensional structure of a human pancreatic ribonuclease variant, a step forward in the design of cytotoxic ribonucleases". Journal of Molecular Biology 303 (1): 49–60. doi:10.1006/jmbi.2000.4506. PMID 11021969.
• Pous J, Mallorquí-Fernández G, Peracaula R, Terzyan SS, Futami J, Tada H, Yamada H, Seno M, de Llorens R, Gomis-Rüth FX, Coll M (Apr 2001). "Three-dimensional structure of human RNase 1 delta N7 at 1.9 A resolution". Acta Crystallographica. Section D, Biological Crystallography 57 (Pt 4): 498–505. doi:10.1107/S0907444901001147. PMID 11264578.
• Gaur D, Swaminathan S, Batra JK (Jul 2001). "Interaction of human pancreatic ribonuclease with human ribonuclease inhibitor. Generation of inhibitor-resistant cytotoxic variants". The Journal of Biological Chemistry 276 (27): 24978–84. doi:10.1074/jbc.M102440200. PMID 11342552.
• Futami J, Maeda T, Kitazoe M, Nukui E, Tada H, Seno M, Kosaka M, Yamada H (Jun 2001). "Preparation of potent cytotoxic ribonucleases by cationization: enhanced cellular uptake and decreased interaction with ribonuclease inhibitor by chemical modification of carboxyl groups". Biochemistry 40 (25): 7518–24. doi:10.1021/bi010248g. PMID 11412105.
• Canals A, Pous J, Guasch A, Benito A, Ribó M, Vilanova M, Coll M (Oct 2001). "The structure of an engineered domain-swapped ribonuclease dimer and its implications for the evolution of proteins toward oligomerization". Structure 9 (10): 967–76. doi:10.1016/S0969-2126(01)00659-1. PMID 11591351.