Calf-intestinal alkaline phosphatase

Calf-intestinal alkaline phosphatase (CIAP/CIP) is a type of alkaline phosphatase that catalyzes the removal of phosphate groups from the 5' end of DNA strands.[1][2] This enzyme is frequently used in DNA sub-cloning, as DNA fragments that lack the 5' phosphate groups cannot ligate. [3] This prevents recircularization of linearized vector DNA and improves the yield of vector containing the appropriate insert.

References

  1. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
  2. Seeburg, P.H. et al. (1977) Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone. Nature 270, 486–94
  3. Ullrich, A. et al. (1977) Rat insulin genes: Construction of plasmids containing the coding sequences. Science 196, 1313–9.



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