Exodeoxyribonuclease I

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Exodeoxyribonuclease I (EC 3.1.11.1, Escherichia coli exonuclease I, E. coli exonuclease I, exonuclease I) is an enzyme.^[1]^[2]^[3] This enzyme catalyses the following chemical reaction

Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates

Preference for single-stranded DNA. The Escherichia coli enzyme hydrolyses glucosylated DNA.

References

↑ Blakesley, R.W., Dodgson, J.B., Nes, I.F. and Wells, R.D. (1977). "Duplex regions in single-stranded phiX174 DNA are cleaved by a restriction endonuclease from Haemophilus aegyptius". J. Biol. Chem. 252: 7300–7306. PMID 71298.
↑ Kelley, R.B., Atkinson, M.R., Huberman, J.A. and Kornberg, A. (1969). "Excision of thymine dimers and other mismatched sequences by DNA polymerases of Escherichia coli". Nature 224: 495–501. doi:10.1038/224495a0.
↑ Lehman, I.R. and Nussbaum, A.L. (1964). "The deoxyribonucleases of Escherichia coli. V. On the specificity of exonuclease I (phosphodiesterase)". J. Biol. Chem. 239: 2628–2636. PMID 14235546.

External links

Exodeoxyribonuclease I at the US National Library of Medicine Medical Subject Headings (MeSH)

Hydrolase: esterases (EC 3.1)

3.1.1: Carboxylic
ester hydrolases

Lipase

Phospholipase
- A1
- A2
- B

3.1.2: Thioesterase

3.1.3: Phosphatase

3.1.4: Phosphodiesterase

3.1.6: Sulfatase

Nuclease (includes
deoxyribonuclease and
ribonuclease)

3.1.11-16: Exonuclease

Exodeoxyribonuclease	RecBCD

Exoribonuclease	Oligonucleotidase

3.1.21-31: Endonuclease

Endodeoxyribonuclease	Deoxyribonuclease I Deoxyribonuclease II Deoxyribonuclease IV Restriction enzyme UvrABC endonuclease

Endoribonuclease	RNase III RNase H 1 2A 2B 2C RNase P RNase A 1 2 3 4/5 RNase T1 RNA-induced silencing complex

either deoxy- or ribo-	Aspergillus nuclease S1 Micrococcal nuclease

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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