Glucosylceramidase

glucosylceramidase
Identifiers
EC number	3.2.1.45
CAS number	37228-64-1
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / EGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, a glucosylceramidase (EC 3.2.1.45) is an enzyme that catalyzes the chemical reaction

D-glucosyl-N-acylsphingosine + H₂O $\rightleftharpoons$ D-glucose + N-acylsphingosine

Thus, the two substrates of this enzyme are D-glucosyl-N-acylsphingosine and H₂O, whereas its two products are D-glucose and N-acylsphingosine.

This enzyme belongs to the family of hydrolases, specifically those glycosidases that hydrolyse O- and S-glycosyl compounds. The systematic name of this enzyme class is D-glucosyl-N-acylsphingosine glucohydrolase. Other names in common use include:

• psychosine hydrolase,
• glucosphingosine glucosylhydrolase,
• GlcCer-beta-glucosidase,
• beta-D-glucocerebrosidase,
• glucosylcerebrosidase,
• beta-glucosylceramidase,
• ceramide glucosidase,
• glucocerebrosidase,
• glucosylsphingosine beta-glucosidase,
• and glucosylsphingosine beta-D-glucosidase.

This enzyme participates in sphingolipid metabolism and degradation of glycan structures.

Human proteins containing this domain

• GBA belongs to Glycoside hydrolase family 30 , GBA2 belongs to Glycoside hydrolase family 116.

References

Further reading

• Brady RO, Kanfer J, and Shapiro D (1965). "The metabolism of glucocerebrosides: I. Purification and properties of a glucocerebroside-cleaving enzyme from spleen tissue". J. Biol. Chem. 240: 39–43.
• Vaccaro AM, Muscillo M, Suzuki K (1985). "Characterization of human glucosylsphingosine glucosyl hydrolase and comparison with glucosylceramidase". Eur. J. Biochem. 146 (2): 315–21. doi:10.1111/j.1432-1033.1985.tb08655.x. PMID 3967661.