NEU3

Sialidase 3 (membrane sialidase)
Identifiers
Symbols	NEU3 ; SIAL3
External IDs	OMIM: 604617 MGI: 1355305 HomoloGene: 4847 ChEMBL: 3046 GeneCards: NEU3 Gene
EC number	3.2.1.18
Gene ontology Molecular function • catalytic activity • hydrolase activity, acting on glycosyl bonds • alpha-sialidase activity • exo-alpha-(2->3)-sialidase activity • exo-alpha-(2->6)-sialidase activity • exo-alpha-(2->8)-sialidase activity Cellular component • plasma membrane • plasma membrane • integral to plasma membrane Biological process • carbohydrate metabolic process • sphingolipid metabolic process • glycosphingolipid metabolic process • ganglioside catabolic process • small molecule metabolic process Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	10825	50877
Ensembl	ENSG00000162139	ENSMUSG00000035239
UniProt	Q9UQ49	Q9JMH7
RefSeq (mRNA)	NM_006656.5	NM_016720.2
RefSeq (protein)	NP_006647.3	NP_057929.1
Location (UCSC)	Chr 11: 74.7 – 74.73 Mb	Chr 7: 106.96 – 106.98 Mb
PubMed search

Sialidase-3 is an enzyme that in humans is encoded by the NEU3 gene.^[1][2]

Function

This gene product belongs to a family of glycohydrolytic enzymes which remove sialic acid residues from glycoproteins and glycolipids. It is localized in the plasma membrane, and its activity is specific for gangliosides. It may play a role in modulating the ganglioside content of the lipid bilayer.^[2]

Interactions

NEU3 has been shown to interact with Grb2.^[3]

References

1. Wada T, Yoshikawa Y, Tokuyama S, Kuwabara M, Akita H, Miyagi T (Aug 1999). "Cloning, expression, and chromosomal mapping of a human ganglioside sialidase". Biochem Biophys Res Commun 261 (1): 21–7. doi:10.1006/bbrc.1999.0973. PMID 10405317.
2. "Entrez Gene: NEU3 sialidase 3 (membrane sialidase)".
3. Sasaki A, Hata K, Suzuki S, Sawada M, Wada T, Yamaguchi K, Obinata M, Tateno H, Suzuki H, Miyagi T (Jul 2003). "Overexpression of plasma membrane-associated sialidase attenuates insulin signaling in transgenic mice". J. Biol. Chem. 278 (30): 27896–902. doi:10.1074/jbc.M212200200. PMID 12730204.

Further reading

• Hu H, Shioda T, Moriya C, Xin X, Hasan MK, Miyake K, Shimada T, Nagai Y (1996). "Infectivities of human and other primate lentiviruses are activated by desialylation of the virion surface". J. Virol. 70 (11): 7462–70. PMC 190813. PMID 8892864.
• Monti E, Bassi MT, Papini N, Riboni M, Manzoni M, Venerando B, Croci G, Preti A, Ballabio A, Tettamanti G, Borsani G (2001). "Identification and expression of NEU3, a novel human sialidase associated to the plasma membrane". Biochem. J. 349 (Pt 1): 343–51. doi:10.1042/0264-6021:3490343. PMC 1221155. PMID 10861246.
• Wang Y, Yamaguchi K, Wada T, Hata K, Zhao X, Fujimoto T, Miyagi T (2002). "A close association of the ganglioside-specific sialidase Neu3 with caveolin in membrane microdomains". J. Biol. Chem. 277 (29): 26252–9. doi:10.1074/jbc.M110515200. PMID 12011038.
• Kakugawa Y, Wada T, Yamaguchi K, Yamanami H, Ouchi K, Sato I, Miyagi T (2002). "Up-regulation of plasma membrane-associated ganglioside sialidase (Neu3) in human colon cancer and its involvement in apoptosis suppression". Proc. Natl. Acad. Sci. U.S.A. 99 (16): 10718–23. doi:10.1073/pnas.152597199. PMC 125023. PMID 12149448.
• Hart ML, Saifuddin M, Uemura K, Bremer EG, Hooker B, Kawasaki T, Spear GT (2003). "High mannose glycans and sialic acid on gp120 regulate binding of mannose-binding lectin (MBL) to HIV type 1". AIDS Res. Hum. Retroviruses 18 (17): 1311–7. doi:10.1089/088922202320886352. PMID 12487819.
• Oehler C, Kopitz J, Cantz M (2003). "Substrate specificity and inhibitor studies of a membrane-bound ganglioside sialidase isolated from human brain tissue". Biol. Chem. 383 (11): 1735–42. doi:10.1515/BC.2002.194. PMID 12530538.
• Sasaki A, Hata K, Suzuki S, Sawada M, Wada T, Yamaguchi K, Obinata M, Tateno H, Suzuki H, Miyagi T (2003). "Overexpression of plasma membrane-associated sialidase attenuates insulin signaling in transgenic mice". J. Biol. Chem. 278 (30): 27896–902. doi:10.1074/jbc.M212200200. PMID 12730204.
• Brandenberger R, Wei H, Zhang S, Lei S, Murage J, Fisk GJ, Li Y, Xu C, Fang R, Guegler K, Rao MS, Mandalam R, Lebkowski J, Stanton LW (2005). "Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation". Nat. Biotechnol. 22 (6): 707–16. doi:10.1038/nbt971. PMID 15146197.
• Ha KT, Lee YC, Cho SH, Kim JK, Kim CH (2004). "Molecular characterization of membrane type and ganglioside-specific sialidase (Neu3) expressed in E. coli". Mol. Cells 17 (2): 267–73. PMID 15179041.
• Stamatos NM, Liang F, Nan X, Landry K, Cross AS, Wang LX, Pshezhetsky AV (2005). "Differential expression of endogenous sialidases of human monocytes during cellular differentiation into macrophages". FEBS J. 272 (10): 2545–56. doi:10.1111/j.1742-4658.2005.04679.x. PMID 15885103.
• Kato K, Shiga K, Yamaguchi K, Hata K, Kobayashi T, Miyazaki K, Saijo S, Miyagi T (2006). "Plasma-membrane-associated sialidase (NEU3) differentially regulates integrin-mediated cell proliferation through laminin- and fibronectin-derived signalling". Biochem. J. 394 (Pt 3): 647–56. doi:10.1042/BJ20050737. PMC 1383714. PMID 16241905.
• Kimura K, Wakamatsu A, Suzuki Y, Ota T, Nishikawa T, Yamashita R, Yamamoto J, Sekine M, Tsuritani K, Wakaguri H, Ishii S, Sugiyama T, Saito K, Isono Y, Irie R, Kushida N, Yoneyama T, Otsuka R, Kanda K, Yokoi T, Kondo H, Wagatsuma M, Murakawa K, Ishida S, Ishibashi T, Takahashi-Fujii A, Tanase T, Nagai K, Kikuchi H, Nakai K, Isogai T, Sugano S (2006). "Diversification of transcriptional modulation: Large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.
• Ueno S, Saito S, Wada T, Yamaguchi K, Satoh M, Arai Y, Miyagi T (2006). "Plasma membrane-associated sialidase is up-regulated in renal cell carcinoma and promotes interleukin-6-induced apoptosis suppression and cell motility". J. Biol. Chem. 281 (12): 7756–64. doi:10.1074/jbc.M509668200. PMID 16428383.
• Yamaguchi K, Hata K, Wada T, Moriya S, Miyagi T (2006). "Epidermal growth factor-induced mobilization of a ganglioside-specific sialidase (NEU3) to membrane ruffles". Biochem. Biophys. Res. Commun. 346 (2): 484–90. doi:10.1016/j.bbrc.2006.05.136. PMID 16765317.
• Nan X, Carubelli I, Stamatos NM (2007). "Sialidase expression in activated human T lymphocytes influences production of IFN-gamma". J. Leukoc. Biol. 81 (1): 284–96. doi:10.1189/jlb.1105692. PMID 17028199.
• Yoshizumi S, Suzuki S, Hirai M, Hinokio Y, Yamada T, Yamada T, Tsunoda U, Aburatani H, Yamaguchi K, Miyagi T, Oka Y (2007). "Increased hepatic expression of ganglioside-specific sialidase, NEU3, improves insulin sensitivity and glucose tolerance in mice". Metab. Clin. Exp. 56 (3): 420–9. doi:10.1016/j.metabol.2006.10.027. PMID 17292733.
• Wada T, Hata K, Yamaguchi K, Shiozaki K, Koseki K, Moriya S, Miyagi T (2007). "A crucial role of plasma membrane-associated sialidase in the survival of human cancer cells". Oncogene 26 (17): 2483–90. doi:10.1038/sj.onc.1210341. PMID 17334392.
• Azuma Y, Sato H, Higai K, Matsumoto K (2007). "Enhanced expression of membrane-associated sialidase Neu3 decreases GD3 and increases GM3 on the surface of Jurkat cells during etoposide-induced apoptosis". Biol. Pharm. Bull. 30 (9): 1680–4. doi:10.1248/bpb.30.1680. PMID 17827720.