Pullulanase
Pullulanase (EC 3.2.1.41, limit dextrinase, amylopectin 6-glucanohydrolase, bacterial debranching enzyme, debranching enzyme, alpha-dextrin endo-1,6-alpha-glucosidase, R-enzyme, pullulan alpha-1,6-glucanohydrolase) is a specific kind of glucanase, an amylolytic exoenzyme, that degrades pullulan.[1][2][3] It is produced as an extracellular, cell surface-anchored lipoprotein by Gram-negative bacteria of the genus Klebsiella. Type I pullulanases specifically attack α-1,6 linkages, while type II pullulanases are also able to hydrolyse α-1,4 linkages. It is also produced by some other bacteria and archaea. Pullulanase is used as a processing aid in grain processing biotechnology (production of ethanol and sweeteners).
Pullulanase is also known as pullulan-6-glucanohydrolase (Debranching enzyme). Its substrate, pullulan, is regarded as a chain of maltotriose units linked by alpha-1,6-glycosidic bonds. Pullulanase will hydrolytically cleave pullulan (alpha-glucan polysaccharides).
References
- ↑ Lee, E.Y.C. and Whelan, W.J. (1972). "Glycogen and starch debranching enzymes". In Boyer, P.D. The Enzymes 5 (3rd ed.). New York: Academic Press. pp. 191–234.
- ↑ Bender, H. and Wallenfels, K. (1966). "Pullulanase (an amylopectin and glycogen debranching enzyme) from Aerobacter aerogenes". Methods Enzymol. Methods in Enzymology 8: 555–559. doi:10.1016/0076-6879(66)08100-X. ISBN 9780121818081.
- ↑ Manners, D.J. (1997). "Observations on the specificity and nomenclature of starch debranching enzymes". J. Appl. Glycosci. 44: 83–85.
External links
|
|---|
| | Activity | |
|---|
| | Regulation | |
|---|
| | Classification | |
|---|
| | Types | |
|---|
|