snRNP70

Small nuclear ribonucleoprotein 70kDa (U1)
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 2L5I​, 2L5J​, 3CW1​, 3PGW​, 4PJO​, 4PKD​
Identifiers
Symbols	SNRNP70 ; RNPU1Z; RPU1; SNRP70; Snp1; U1-70K; U170K; U1AP; U1RNP
External IDs	OMIM: 180740 MGI: 98341 HomoloGene: 20672 GeneCards: SNRNP70 Gene
Gene ontology Molecular function • nucleotide binding • RNA binding • protein binding • poly(A) RNA binding Cellular component • nucleus • nucleoplasm • spliceosomal complex • U1 snRNP • nuclear speck Biological process • mRNA splicing, via spliceosome • mRNA processing • RNA splicing • gene expression • regulation of RNA splicing • positive regulation of mRNA splicing, via spliceosome Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	6625	20637
Ensembl	ENSG00000104852	ENSMUSG00000063511
UniProt	P08621	Q62376
RefSeq (mRNA)	NM_001009820	NM_009224
RefSeq (protein)	NP_001287998	NP_033250
Location (UCSC)	Chr 19: 49.09 – 49.11 Mb	Chr 7: 45.38 – 45.4 Mb
PubMed search

snRNP70 also known as U1 small nuclear ribonucleoprotein 70 kDa is a protein^[1] that in humans is encoded by the SNRNP70 gene.^[2][3] snRNP70 is a small nuclear ribonucleoprotein that associates with U1 spliceosomal RNA, forming part of the spliceosome.

Interactions

snRNP70 has been shown to interact with ASF/SF2,^[4][5] SRPK1,^[6][7] and ZRANB2.^[8]

Role in autoimmunity

Antibodies towards snRNP70 are associated with mixed connective tissue disease.^[9]

References

1. Spritz RA, Strunk K, Surowy CS, Hoch SO, Barton DE, Francke U (December 1987). "The human U1-70K snRNP protein: cDNA cloning, chromosomal localization, expression, alternative splicing and RNA-binding". Nucleic Acids Res. 15 (24): 10373–91. doi:10.1093/nar/15.24.10373. PMC 339950. PMID 2447561.
2. "Entrez Gene: SNRP70 small nuclear ribonucleoprotein 70kDa polypeptide (RNP antigen)".
3. Spritz RA, Strunk K, Surowy CS, Mohrenweiser HW (October 1990). "Human U1-70K ribonucleoprotein antigen gene: organization, nucleotide sequence, and mapping to locus 19q13.3". Genomics 8 (2): 371–9. doi:10.1016/0888-7543(90)90295-6. PMID 2147422.
4. Xiao SH, Manley JL (November 1998). "Phosphorylation-dephosphorylation differentially affects activities of splicing factor ASF/SF2". EMBO J. 17 (21): 6359–67. doi:10.1093/emboj/17.21.6359. PMC 1170960. PMID 9799243.
5. Cao W, Garcia-Blanco MA (August 1998). "A serine/arginine-rich domain in the human U1 70k protein is necessary and sufficient for ASF/SF2 binding". J. Biol. Chem. 273 (32): 20629–35. doi:10.1074/jbc.273.32.20629. PMID 9685421.
6. Wang HY, Lin W, Dyck JA, Yeakley JM, Songyang Z, Cantley LC, Fu XD (February 1998). "SRPK2: a differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells". J. Cell Biol. 140 (4): 737–50. doi:10.1083/jcb.140.4.737. PMC 2141757. PMID 9472028.
7. Kamachi M, Le TM, Kim SJ, Geiger ME, Anderson P, Utz PJ (November 2002). "Human autoimmune sera as molecular probes for the identification of an autoantigen kinase signaling pathway". J. Exp. Med. 196 (9): 1213–25. doi:10.1084/jem.20021167. PMC 2194102. PMID 12417631.
8. Adams DJ, van der Weyden L, Mayeda A, Stamm S, Morris BJ, Rasko JE (July 2001). "ZNF265--a novel spliceosomal protein able to induce alternative splicing". J. Cell Biol. 154 (1): 25–32. doi:10.1083/jcb.200010059. PMC 2196870. PMID 11448987.
9. Sato, T; Fujii, T; Yokoyama, T; Fujita, Y; Imura, Y; Yukawa, N; Kawabata, D; Nojima, T; Ohmura, K; Usui, T; Mimori, T (Dec 2010). "Anti-U1 RNP antibodies in cerebrospinal fluid are associated with central neuropsychiatric manifestations in systemic lupus erythematosus and mixed connective tissue disease.". Arthritis and rheumatism 62 (12): 3730–40. doi:10.1002/art.27700. PMID 20722023.

Further reading

• Wilk HE, Schaefer KP, Agris PF, et al. (1991). "U1 SnRNP association with HnRNP involves an initial non-specific splice-site independent interaction of U1 SnRNP protein with HnRNA.". Mol. Cell. Biochem. 106 (1): 55–66. doi:10.1007/BF00231189. PMID 1833625.
• Query CC, Bentley RC, Keene JD (1989). "A common RNA recognition motif identified within a defined U1 RNA binding domain of the 70K U1 snRNP protein.". Cell 57 (1): 89–101. doi:10.1016/0092-8674(89)90175-X. PMID 2467746.
• Du Clos TW (1989). "C-reactive protein reacts with the U1 small nuclear ribonucleoprotein.". J. Immunol. 143 (8): 2553–9. PMID 2477447.
• Theissen H, Etzerodt M, Reuter R, et al. (1987). "Cloning of the human cDNA for the U1 RNA-associated 70K protein.". EMBO J. 5 (12): 3209–17. PMC 1167314. PMID 3028775.
• Romac JM, Graff DH, Keene JD (1994). "The U1 small nuclear ribonucleoprotein (snRNP) 70K protein is transported independently of U1 snRNP particles via a nuclear localization signal in the RNA-binding domain.". Mol. Cell. Biol. 14 (7): 4662–70. PMC 358839. PMID 7516470.
• Wu JY, Maniatis T (1994). "Specific interactions between proteins implicated in splice site selection and regulated alternative splicing.". Cell 75 (6): 1061–70. doi:10.1016/0092-8674(93)90316-I. PMID 8261509.
• Woppmann A, Will CL, Kornstädt U, et al. (1993). "Identification of an snRNP-associated kinase activity that phosphorylates arginine/serine rich domains typical of splicing factors.". Nucleic Acids Res. 21 (12): 2815–22. doi:10.1093/nar/21.12.2815. PMC 309659. PMID 8332490.
• Northemann W, Berg H, Stahnke G, et al. (1996). "Identification of an inhibitory element within the human 68-kDa (U1) ribonucleoprotein antigen.". Protein Expr. Purif. 6 (6): 748–56. doi:10.1006/prep.1995.0005. PMID 8746626.
• Zhang WJ, Wu JY (1996). "Functional properties of p54, a novel SR protein active in constitutive and alternative splicing.". Mol. Cell. Biol. 16 (10): 5400–8. PMC 231539. PMID 8816452.
• Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery.". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
• Hong W, Bennett M, Xiao Y, et al. (1997). "Association of U2 snRNP with the spliceosomal complex E.". Nucleic Acids Res. 25 (2): 354–61. doi:10.1093/nar/25.2.354. PMC 146436. PMID 9016565.
• Klein Gunnewiek JM, van Aarssen Y, van der Kemp A, et al. (1997). "Nuclear accumulation of the U1 snRNP-specific protein C is due to diffusion and retention in the nucleus.". Exp. Cell Res. 235 (1): 265–73. doi:10.1006/excr.1997.3663. PMID 9281376.
• Zhang WJ, Wu JY (1998). "Sip1, a novel RS domain-containing protein essential for pre-mRNA splicing.". Mol. Cell. Biol. 18 (2): 676–84. PMC 108778. PMID 9447963.
• Blencowe BJ, Issner R, Nickerson JA, Sharp PA (1998). "A coactivator of pre-mRNA splicing.". Genes Dev. 12 (7): 996–1009. doi:10.1101/gad.12.7.996. PMC 316672. PMID 9531537.
• Cao W, Garcia-Blanco MA (1998). "A serine/arginine-rich domain in the human U1 70k protein is necessary and sufficient for ASF/SF2 binding.". J. Biol. Chem. 273 (32): 20629–35. doi:10.1074/jbc.273.32.20629. PMID 9685421.
• Neubauer G, King A, Rappsilber J, et al. (1998). "Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex.". Nat. Genet. 20 (1): 46–50. doi:10.1038/1700. PMID 9731529.
• Xiao SH, Manley JL (1999). "Phosphorylation-dephosphorylation differentially affects activities of splicing factor ASF/SF2.". EMBO J. 17 (21): 6359–67. doi:10.1093/emboj/17.21.6359. PMC 1170960. PMID 9799243.
• Dumortier H, Klein Gunnewiek J, Roussel JP, et al. (1999). "At least three linear regions but not the zinc-finger domain of U1C protein are exposed at the surface of the protein in solution and on the human spliceosomal U1 snRNP particle.". Nucleic Acids Res. 26 (23): 5486–91. doi:10.1093/nar/26.23.5486. PMC 147990. PMID 9826776.