Arabinogalactan endo-1,4-beta-galactosidase

Arabinogalactan endo-beta-1,4-galactanase (EC 3.2.1.89, endo-1,4-beta-galactanase, galactanase, arabinogalactanase, ganB (gene)) is an enzyme with systematic name arabinogalactan 4-beta-D-galactanohydrolase.^[1]^[2]^[3] This enzyme catalyses the following chemical reaction

The enzyme specifically hydrolyses (1->4)-beta-D-galactosidic linkages in type I arabinogalactans.

This enzyme is isolated from the bacterium Bacillus subtilis.

References

↑ Emi, S. and Yamamoto, T. (1972). "Purification and properties of several galactanases of Bacillus subtilis var. amylosacchariticus". Agric. Biol. Chem. 36: 1945–1954. doi:10.1271/bbb1961.36.1945.
↑ Labavitch, J.M., Freeman, L.E. and Albersheim, P. (1976). "Structure of plant cell walls. Purification and characterization of a β-1,4-galactanase which degrades a structural component of the primary cell walls of dicots". J. Biol. Chem. 251: 5904–5910. PMID 823153.
↑ Shipkowski, S. and Brenchley, J.E. (2006). "Bioinformatic, genetic, and biochemical evidence that some glycoside hydrolase family 42 β-galactosidases are arabinogalactan type I oligomer hydrolases". Appl. Environ. Microbiol. 72: 7730–7738. doi:10.1128/aem.01306-06. PMID 17056685.

External links

Arabinogalactan endo-beta-1,4-galactanase at the US National Library of Medicine Medical Subject Headings (MeSH)

Hydrolase: sugar hydrolases (EC 3.2)

3.2.1: Glycoside hydrolases

Disaccharidase	Sucrase/Sucrase-isomaltase/Invertase Maltase Trehalase Lactase

Glucosidases	Cellulase Alpha-glucosidase Acid Neutral AB Neutral C Beta-glucosidase cytosolic Debranching enzyme

Other	Amylase Alpha-amylase Chitinase Lysozyme Neuraminidase NEU1 NEU2 NEU3 NEU4 Bacterial neuraminidase Viral neuraminidase Galactosidases Alpha Beta alpha-Mannosidase Glucuronidase Hyaluronidase Pullulanase Glucosylceramidase lysosomal non-lysosomal Galactosylceramidase Alpha-N-acetylgalactosaminidase NAGA Alpha-N-acetylglucosaminidase Fucosidase Hexosaminidase HEXA HEXB Iduronidase Maltase-glucoamylase Heparanase HPSE2

3.2.2: Hydrolysing
N-Glycosyl compounds

DNA glycosylases: Oxoguanine glycosylase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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