CYP2C18

Cytochrome P450, family 2, subfamily C, polypeptide 18
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 2CIK​, 2H6P​
Identifiers
Symbols	CYP2C18 ; CPCI; CYP2C; CYP2C17; P450-6B/29C; P450IIC17
External IDs	OMIM: 601131 MGI: 1919332 HomoloGene: 133567 ChEMBL: 2408 GeneCards: CYP2C18 Gene
EC number	1.14.14.1
Gene ontology Molecular function • monooxygenase activity • iron ion binding • arachidonic acid epoxygenase activity • steroid hydroxylase activity • oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen • oxygen binding • heme binding • aromatase activity Cellular component • cytoplasm • endoplasmic reticulum membrane • intracellular membrane-bounded organelle Biological process • xenobiotic metabolic process • epoxygenase P450 pathway • exogenous drug catabolic process • small molecule metabolic process • oxidation-reduction process Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	1562	72082
Ensembl	ENSG00000108242	ENSMUSG00000025002
UniProt	P33260	Q9D816
RefSeq (mRNA)	NM_000772	NM_028089
RefSeq (protein)	NP_000763	NP_082365
Location (UCSC)	Chr 10: 94.68 – 94.74 Mb	Chr 19: 39.01 – 39.04 Mb
PubMed search

Cytochrome P450 2C18 is a protein that in humans is encoded by the CYP2C18 gene.^[1][2][3]

Function

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum but its specific substrate has not yet been determined. The gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. An additional gene, CYP2C17, was once thought to exist; however, CYP4217 is now considered an artefact based on a chimera of CYP2C18 and CYP2C19.^[3]

CYP2C18 also possesses epoxygenase activitiy: it can attack various long-chain polyunsaturated fatty acids at their double (i.e. alkene) bonds to form epoxide products that act as signaling agents. It metabolizes: 1) arachidonic acid to various epoxyeicosatrienoic acids (also termed EETs); 2) linoleic acid to 9,10-epoxy octadecaenoic acids (also termed vernolic acid, linoleic acid 9:10-oxide, or leukotoxin) and 12,13-epoxy-octadecaenoic (also termed coronaric acid, linoleic acid 12,13-oxide, or isoleukotoxin); 3) docosohexaenoic acid to various epoxydocosapentaenoic acids (also termed EDPs); and 4) eicosapentaenoic acid to various epoxyeicosatetraenoic acids (also termed EEQs).^[4][5][6]

While CYP2C19, CYP2C8, CYP2C9, CYP2J2, and possibly CYP2S1 are the main producers of EETs and, very likely EEQs, EDPs, and the epoxides of linoleic acid, CYP2C18 may contribute to the production of these metabolites in certain tissues.^[7][8]

References

1. Furuya H, Meyer UA, Gelboin HV, Gonzalez FJ (September 1991). "Polymerase chain reaction-directed identification, cloning, and quantification of human CYP2C18 mRNA". Molecular Pharmacology 40 (3): 375–82. PMID 1896026.
2. Romkes M, Faletto MB, Blaisdell JA, Raucy JL, Goldstein JA (April 1991). "Cloning and expression of complementary DNAs for multiple members of the human cytochrome P450IIC subfamily". Biochemistry 30 (13): 3247–55. doi:10.1021/bi00227a012. PMID 2009263.
3. "Entrez Gene: CYP2C18 cytochrome P450, family 2, subfamily C, polypeptide 18".
4. Fleming I (October 2014). "The pharmacology of the cytochrome P450 epoxygenase/soluble epoxide hydrolase axis in the vasculature and cardiovascular disease". Pharmacological Reviews 66 (4): 1106–40. doi:10.1124/pr.113.007781. PMID 25244930.
5. Wagner K, Vito S, Inceoglu B, Hammock BD (October 2014). "The role of long chain fatty acids and their epoxide metabolites in nociceptive signaling". Prostaglandins & Other Lipid Mediators. 113-115: 2–12. doi:10.1016/j.prostaglandins.2014.09.001. PMID 25240260.
6. Fischer R, Konkel A, Mehling H, Blossey K, Gapelyuk A, Wessel N, von Schacky C, Dechend R, Muller DN, Rothe M, Luft FC, Weylandt K, Schunck WH (March 2014). "Dietary omega-3 fatty acids modulate the eicosanoid profile in man primarily via the CYP-epoxygenase pathway". Journal of Lipid Research 55 (6): 1150–1164. doi:10.1194/jlr.M047357. PMID 24634501.
7. Wagner K, Vito S, Inceoglu B, Hammock BD (October 2014). "The role of long chain fatty acids and their epoxide metabolites in nociceptive signaling". Prostaglandins & Other Lipid Mediators. 113-115: 2–12. doi:10.1016/j.prostaglandins.2014.09.001. PMID 25240260.
8. Spector AA, Kim HY (April 2015). "Cytochrome P450 epoxygenase pathway of polyunsaturated fatty acid metabolism". Biochimica et Biophysica Acta 1851 (4): 356–65. doi:10.1016/j.bbalip.2014.07.020. PMID 25093613.

Further reading

• Goldstein JA, de Morais SM (December 1994). "Biochemistry and molecular biology of the human CYP2C subfamily". Pharmacogenetics 4 (6): 285–99. doi:10.1097/00008571-199412000-00001. PMID 7704034.
• Smith G, Stubbins MJ, Harries LW, Wolf CR (December 1998). "Molecular genetics of the human cytochrome P450 monooxygenase superfamily". Xenobiotica; The Fate of Foreign Compounds in Biological Systems 28 (12): 1129–65. doi:10.1080/004982598238868. PMID 9890157.
• Ged C, Beaune P (June 1992). "Partial sequence and polymerase chain reaction-mediated analysis of expression of the human CYP2C18 gene". Pharmacogenetics 2 (3): 109–15. doi:10.1097/00008571-199206000-00002. PMID 1306110.
• Romkes M, Faletto MB, Blaisdell JA, Raucy JL, Goldstein JA (February 1993). "Cloning and expression of complementary DNAs for multiple members of the human cytochrome PH50IIC subfamily". Biochemistry 32 (5): 1390. doi:10.1021/bi00056a025. PMID 8095407.
• Goldstein JA, Faletto MB, Romkes-Sparks M, Sullivan T, Kitareewan S, Raucy JL, Lasker JM, Ghanayem BI (February 1994). "Evidence that CYP2C19 is the major (S)-mephenytoin 4'-hydroxylase in humans". Biochemistry 33 (7): 1743–52. doi:10.1021/bi00173a017. PMID 8110777.
• Maruyama K, Sugano S (January 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene 138 (1-2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• de Morais SM, Schweikl H, Blaisdell J, Goldstein JA (July 1993). "Gene structure and upstream regulatory regions of human CYP2C9 and CYP2C18". Biochemical and Biophysical Research Communications 194 (1): 194–201. doi:10.1006/bbrc.1993.1803. PMID 8333835.
• Richardson TH, Griffin KJ, Jung F, Raucy JL, Johnson EF (February 1997). "Targeted antipeptide antibodies to cytochrome P450 2C18 based on epitope mapping of an inhibitory monoclonal antibody to P450 2C51". Archives of Biochemistry and Biophysics 338 (2): 157–64. doi:10.1006/abbi.1996.9817. PMID 9028867.
• Zaphiropoulos PG (June 1997). "Exon skipping and circular RNA formation in transcripts of the human cytochrome P-450 2C18 gene in epidermis and of the rat androgen binding protein gene in testis". Molecular and Cellular Biology 17 (6): 2985–93. PMC 232150. PMID 9154796.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (October 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1-2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Macé K, Bowman ED, Vautravers P, Shields PG, Harris CC, Pfeifer AM (May 1998). "Characterisation of xenobiotic-metabolising enzyme expression in human bronchial mucosa and peripheral lung tissues". European Journal of Cancer 34 (6): 914–20. doi:10.1016/S0959-8049(98)00034-3. PMID 9797707.
• Klose TS, Blaisdell JA, Goldstein JA (1999). "Gene structure of CYP2C8 and extrahepatic distribution of the human CYP2Cs". Journal of Biochemical and Molecular Toxicology 13 (6): 289–95. doi:10.1002/(SICI)1099-0461(1999)13:6<289::AID-JBT1>3.0.CO;2-N. PMID 10487415.
• Finta C, Zaphiropoulos PG (February 2000). "The human CYP2C locus: a prototype for intergenic and exon repetition splicing events". Genomics 63 (3): 433–8. doi:10.1006/geno.1999.6063. PMID 10704292.
• Thum T, Borlak J (March 2000). "Gene expression in distinct regions of the heart". Lancet 355 (9208): 979–83. doi:10.1016/S0140-6736(00)99016-0. PMID 10768437.
• Marill J, Cresteil T, Lanotte M, Chabot GG (December 2000). "Identification of human cytochrome P450s involved in the formation of all-trans-retinoic acid principal metabolites". Molecular Pharmacology 58 (6): 1341–8. PMID 11093772.
• Zhu-Ge J, Yu YN, Qian YL, Li X (October 2002). "Establishment of a transgenic cell line stably expressing human cytochrome P450 2C18 and identification of a CYP2C18 clone with exon 5 missing". World Journal of Gastroenterology 8 (5): 888–92. PMID 12378636.