Centromere protein B

Centromere protein B, 80kDa

PDB rendering based on 1bw6.

Available structures

Ortholog search: PDBe, RCSB

List of PDB id codes
1BW6, 1HLV, 1UFI

Identifiers

Symbol

CENPB

External IDs

OMIM: 117140 MGI: 88376 HomoloGene: 1370 GeneCards: CENPB Gene

Gene ontology
Molecular function	• chromatin binding • satellite DNA binding • centromeric DNA binding • sequence-specific DNA binding
Cellular component	• chromosome, centromeric region • nucleus • chromosome
Biological process	• regulation of transcription, DNA-templated
Sources: Amigo / QuickGO

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 20:
3.78 – 3.79 Mb

Chr 2:
131.18 – 131.18 Mb

PubMed search

Centromere protein B also known as major centromere autoantigen B is an autoantigen protein of the cell nucleus. In humans, centromere protein B is encoded by the CENPB gene.^[1]^[2]^[3]

Function

Centromere protein B is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.

Clinical significance

Centromere protein B is a potential biomarker of small-cell lung cancer.^[4]

References

↑ "Entrez Gene: centromere protein B".
↑ Sugimoto K, Yata H, Himeno M (July 1993). "Mapping of the human CENP-B gene to chromosome 20 and the CENP-C gene to chromosome 12 by a rapid cycle DNA amplification procedure". Genomics 17 (1): 240–2. doi:10.1006/geno.1993.1312. PMID 8406460.
↑ Ando S, Yang H, Nozaki N, Okazaki T, Yoda K (April 2002). "CENP-A, -B, and -C Chromatin Complex That Contains the I-Type α-Satellite Array Constitutes the Prekinetochore in HeLa Cells". Mol. Cell. Biol. 22 (7): 2229–41. doi:10.1128/MCB.22.7.2229-2241.2002. PMC 133672. PMID 11884609.
↑ Briasoulis E, Kamposioras K, Tzovaras V, Pafitanis G, Kostoula A, Mavridis A, Pavlidis N (May 2008). "CENP-B specific anti-centromere autoantibodies heralding small-cell lung cancer. A case study and review of the literature". Lung Cancer 60 (2): 302–6. doi:10.1016/j.lungcan.2007.09.014. PMID 17980453.

Saxena A, Wong LH, Kalitsis P, et al. (2002). "Poly(ADP-ribose) polymerase 2 localizes to mammalian active centromeres and interacts with PARP-1, Cenpa, Cenpb and Bub3, but not Cenpc". Hum. Mol. Genet. 11 (19): 2319–29. doi:10.1093/hmg/11.19.2319. PMID 12217960.
Nousiainen M, Silljé HH, Sauer G, et al. (2006). "Phosphoproteome analysis of the human mitotic spindle". Proc. Natl. Acad. Sci. U.S.A. 103 (14): 5391–6. doi:10.1073/pnas.0507066103. PMC 1459365. PMID 16565220.
Orthaus S, Biskup C, Hoffmann B, et al. (2008). "Assembly of the inner kinetochore proteins CENP-A and CENP-B in living human cells". Chembiochem 9 (1): 77–92. doi:10.1002/cbic.200700358. PMID 18072184.
Tawaramoto MS, Park SY, Tanaka Y, et al. (2003). "Crystal structure of the human centromere protein B (CENP-B) dimerization domain at 1.65-A resolution". J. Biol. Chem. 278 (51): 51454–61. doi:10.1074/jbc.M310388200. PMID 14522975.
Tanaka Y, Tachiwana H, Yoda K, et al. (2005). "Human centromere protein B induces translational positioning of nucleosomes on alpha-satellite sequences". J. Biol. Chem. 280 (50): 41609–18. doi:10.1074/jbc.M509666200. PMID 16183641.
Wellcome Trust Case Control Consortium; Clayton, David G.; Cardon, Lon R.; Craddock, Nick; Deloukas, Panos; Duncanson, Audrey; Kwiatkowski, Dominic P.; McCarthy, Mark I.; et al. (2007). "Genome-wide association study of 14,000 cases of seven common diseases and 3,000 shared controls". Nature 447 (7145): 661–78. doi:10.1038/nature05911. PMC 2719288. PMID 17554300.
Tanaka Y, Kurumizaka H, Yokoyama S (2005). "CpG methylation of the CENP-B box reduces human CENP-B binding". FEBS J. 272 (1): 282–9. doi:10.1111/j.1432-1033.2004.04406.x. PMID 15634350.
Sowa ME, Bennett EJ, Gygi SP, Harper JW (2009). "Defining the Human Deubiquitinating Enzyme Interaction Landscape". Cell 138 (2): 389–403. doi:10.1016/j.cell.2009.04.042. PMC 2716422. PMID 19615732.
Lim J, Hao T, Shaw C, et al. (2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell 125 (4): 801–14. doi:10.1016/j.cell.2006.03.032. PMID 16713569.
Lomonte P, Morency E (2007). "Centromeric protein CENP-B proteasomal degradation induced by the viral protein ICP0". FEBS Lett. 581 (4): 658–62. doi:10.1016/j.febslet.2007.01.027. PMID 17258208.
Suzuki N, Nakano M, Nozaki N, et al. (2004). "CENP-B interacts with CENP-C domains containing Mif2 regions responsible for centromere localization". J. Biol. Chem. 279 (7): 5934–46. doi:10.1074/jbc.M306477200. PMID 14612452.
Hellwig D, Münch S, Orthaus S, et al. (2008). "Live-cell imaging reveals sustained centromere binding of CENP-T via CENP-A and CENP-B". J Biophotonics 1 (3): 245–54. doi:10.1002/jbio.200810014. PMID 19412974.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Robitaille G, Hénault J, Christin MS, et al. (2007). "The nuclear autoantigen CENP-B displays cytokine-like activities toward vascular smooth muscle cells". Arthritis Rheum. 56 (11): 3814–26. doi:10.1002/art.22972. PMID 17968937.
Obuse C, Yang H, Nozaki N, et al. (2004). "Proteomics analysis of the centromere complex from HeLa interphase cells: UV-damaged DNA binding protein 1 (DDB-1) is a component of the CEN-complex, while BMI-1 is transiently co-localized with the centromeric region in interphase". Genes Cells 9 (2): 105–20. doi:10.1111/j.1365-2443.2004.00705.x. PMID 15009096.
Perdoni F, Bottone MG, Soldani C, Alpini C, Pellicciari C, Scovassi AI, et al. (2009). "Distribution of centromeric proteins and PARP-1 during mitosis and apoptosis". Ann. N. Y. Acad. Sci. 1171: 32–7. doi:10.1111/j.1749-6632.2009.04729.x. PMID 19723035. Missing |last4= in Authors list (help)
Andersen JS, Lam YW, Leung AK, et al. (2005). "Nucleolar proteome dynamics". Nature 433 (7021): 77–83. doi:10.1038/nature03207. PMID 15635413.
Strausberg RL, Feingold EA, Grouse LH, et al. (2002). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Robitaille G, Christin MS, Clément I, et al. (2009). "Nuclear autoantigen CENP-B transactivation of the epidermal growth factor receptor via chemokine receptor 3 in vascular smooth muscle cells". Arthritis Rheum. 60 (9): 2805–16. doi:10.1002/art.24765. PMID 19714638.