Double-stranded uracil-DNA glycosylase

Double-stranded uracil-DNA glycosylase (EC 3.2.2.28, Mug, double-strand uracil-DNA glycosylase, Dug, dsUDG, double-stranded DNA specific UDG, dsDNA specific UDG, UdgB, G:T/U mismatch-specific DNA glycosylase, UDG) is an enzyme with systematic name uracil-double-stranded DNA deoxyribohydrolase (uracil-releasing).^[1]^[2]^[3] This enzyme catalyses the following chemical reaction

Specifically hydrolyses mismatched double-stranded DNA and polynucleotides, releasing free uracil

This enzyme is not active on DNA containing a T/G mispair or single-stranded DNA.

References

↑ Barrett, T.E., Scharer, O.D., Savva, R., Brown, T., Jiricny, J., Verdine, G.L. and Pearl, L.H. (1999). "Crystal structure of a thwarted mismatch glycosylase DNA repair complex". EMBO J. 18: 6599–6609. doi:10.1093/emboj/18.23.6599. PMID 10581234.
↑ Sung, J.S. and Mosbaugh, D.W. (2000). "Escherichia coli double-strand uracil-DNA glycosylase: involvement in uracil-mediated DNA base excision repair and stimulation of activity by endonuclease IV". Biochemistry 39: 10224–10235. doi:10.1021/bi0007066. PMID 10956012.
↑ Mokkapati, S.K., Fernandez de Henestrosa, A.R. and Bhagwat, A.S. (2001). "Escherichia coli DNA glycosylase Mug: a growth-regulated enzyme required for mutation avoidance in stationary-phase cells". Mol. Microbiol. 41: 1101–1111. doi:10.1046/j.1365-2958.2001.02559.x. PMID 11555290.

External links

Double-stranded uracil-DNA glycosylase at the US National Library of Medicine Medical Subject Headings (MeSH)

Hydrolase: sugar hydrolases (EC 3.2)

3.2.1: Glycoside hydrolases

Disaccharidase	Sucrase/Sucrase-isomaltase/Invertase Maltase Trehalase Lactase

Glucosidases	Cellulase Alpha-glucosidase Acid Neutral AB Neutral C Beta-glucosidase cytosolic Debranching enzyme

Other	Amylase Alpha-amylase Chitinase Lysozyme Neuraminidase NEU1 NEU2 NEU3 NEU4 Bacterial neuraminidase Viral neuraminidase Galactosidases Alpha Beta alpha-Mannosidase Glucuronidase Hyaluronidase Pullulanase Glucosylceramidase lysosomal non-lysosomal Galactosylceramidase Alpha-N-acetylgalactosaminidase NAGA Alpha-N-acetylglucosaminidase Fucosidase Hexosaminidase HEXA HEXB Iduronidase Maltase-glucoamylase Heparanase HPSE2

3.2.2: Hydrolysing
N-Glycosyl compounds

DNA glycosylases: Oxoguanine glycosylase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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