Exo-1,4-beta-D-glucosaminidase

Exo-1,4-beta-D-glucosaminidase (EC 3.2.1.165, CsxA, GlcNase, exochitosanase, GlmA, exo-beta-D-glucosaminidase, chitosan exo-1,4-beta-D-glucosaminidase) is an enzyme with systematic name chitosan exo-(1->4)-beta-D-glucosaminidase.^[1]^[2]^[3]^[4]^[5] This enzyme catalyses the following chemical reaction

Hydrolysis of chitosan or chitosan oligosaccharides to remove successive D-glucosamine residues from the non-reducing termini

Chitosan is a partially or totally N-deacetylated chitin derivative that is found in the cell walls of some phytopathogenic fungi .

References

↑ Nanjo, F., Katsumi, R. and Sakai, K. (1990). "Purification and characterization of an exo-β-D-glucosaminidase, a novel type of enzyme, from Nocardia orientalis". J. Biol. Chem. 265: 10088–10094. PMID 2351651.
↑ Nogawa, M., Takahashi, H., Kashiwagi, A., Ohshima, K., Okada, H. and Morikawa, Y. (1998). "Purification and characterization of exo-β-D-glucosaminidase from a cellulolytic fungus, Trichoderma reesei PC-3-7". Appl. Environ. Microbiol. 64: 890–895. PMID 16349528.
↑ Fukamizo, T., Fleury, A., Côté, N., Mitsutomi, M. and Brzezinski, R. (2006). "Exo-β-D-glucosaminidase from Amycolatopsis orientalis: catalytic residues, sugar recognition specificity, kinetics, and synergism". Glycobiology 16: 1064–1072. doi:10.1093/glycob/cwl026. PMID 16877749.
↑ Côté, N., Fleury, A., Dumont-Blanchette, E., Fukamizo, T., Mitsutomi, M. and Brzezinski, R. (2006). "Two exo-β-D-glucosaminidases/exochitosanases from actinomycetes define a new subfamily within family 2 of glycoside hydrolases". Biochem. J. 394: 675–686. doi:10.1042/bj20051436. PMID 16316314.
↑ Ike, M., Isami, K., Tanabe, Y., Nogawa, M., Ogasawara, W., Okada, H. and Morikawa, Y. (2006). "Cloning and heterologous expression of the exo-β-D-glucosaminidase-encoding gene (gls93) from a filamentous fungus, Trichoderma reesei PC-3-7". Appl. Microbiol. Biotechnol. 72: 687–695. doi:10.1007/s00253-006-0320-y. PMID 16636831.

External links

Exo-1,4-beta-D-glucosaminidase at the US National Library of Medicine Medical Subject Headings (MeSH)

Hydrolase: sugar hydrolases (EC 3.2)

3.2.1: Glycoside hydrolases

Disaccharidase	Sucrase/Sucrase-isomaltase/Invertase Maltase Trehalase Lactase

Glucosidases	Cellulase Alpha-glucosidase Acid Neutral AB Neutral C Beta-glucosidase cytosolic Debranching enzyme

Other	Amylase Alpha-amylase Chitinase Lysozyme Neuraminidase NEU1 NEU2 NEU3 NEU4 Bacterial neuraminidase Viral neuraminidase Galactosidases Alpha Beta alpha-Mannosidase Glucuronidase Hyaluronidase Pullulanase Glucosylceramidase lysosomal non-lysosomal Galactosylceramidase Alpha-N-acetylgalactosaminidase NAGA Alpha-N-acetylglucosaminidase Fucosidase Hexosaminidase HEXA HEXB Iduronidase Maltase-glucoamylase Heparanase HPSE2

3.2.2: Hydrolysing
N-Glycosyl compounds

DNA glycosylases: Oxoguanine glycosylase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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