Galactan endo-1,6-beta-galactosidase

Galactan endo-1,6-beta-galactosidase (EC 3.2.1.164, endo-1,6-beta-galactanase) is an enzyme with systematic name endo-beta-(1->6)-galactanase.^[1]^[2]^[3] This enzyme catalyses the following chemical reaction

Endohydrolysis of (1->6)-beta-D-galactosidic linkages in arabinogalactan proteins and (1->3):(1->6)-beta-galactans to yield galactose and (1->6)-beta-galactobiose as the final products

The enzyme specifically hydrolyses 1,6-beta-D-galactooligosaccharides with a degree of polymerization (DP) higher than 3.

References

↑ Brillouet, J.-M., Williams, P. and Moutounet, M. (1991). "Purification and some properties of a novel endo-β-(1→6)-D-galactanase from Aspergillus niger". Agric. Biol. Chem. 55: 1565–1571. doi:10.1271/bbb1961.55.1565.
↑ Okemoto, K., Uekita, T., Tsumuraya, Y., Hashimoto, Y. and Kasama, T. (2003). "Purification and characterization of an endo-β-(1→6)-galactanase from Trichoderma viride". Carbohydr. Res. 338 (3): 219–230. doi:10.1016/s0008-6215(02)00405-6. PMID 12543554.
↑ Kotake, T., Kaneko, S., Kubomoto, A., Haque, M.A., Kobayashi, H. and Tsumuraya, Y. (2004). "Molecular cloning and expression in Escherichia coli of a Trichoderma viride endo-β-(1→6)-galactanase gene". Biochem. J. 377 (Pt 3): 749–755. doi:10.1042/BJ20031145. PMID 14565843.

External links

Galactan endo-1,6-beta-galactosidase at the US National Library of Medicine Medical Subject Headings (MeSH)

Hydrolase: sugar hydrolases (EC 3.2)

3.2.1: Glycoside hydrolases

Disaccharidase	Sucrase/Sucrase-isomaltase/Invertase Maltase Trehalase Lactase

Glucosidases	Cellulase Alpha-glucosidase Acid Neutral AB Neutral C Beta-glucosidase cytosolic Debranching enzyme

Other	Amylase Alpha-amylase Chitinase Lysozyme Neuraminidase NEU1 NEU2 NEU3 NEU4 Bacterial neuraminidase Viral neuraminidase Galactosidases Alpha Beta alpha-Mannosidase Glucuronidase Hyaluronidase Pullulanase Glucosylceramidase lysosomal non-lysosomal Galactosylceramidase Alpha-N-acetylgalactosaminidase NAGA Alpha-N-acetylglucosaminidase Fucosidase Hexosaminidase HEXA HEXB Iduronidase Maltase-glucoamylase Heparanase HPSE2

3.2.2: Hydrolysing
N-Glycosyl compounds

DNA glycosylases: Oxoguanine glycosylase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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