Protein O-GlcNAcase

Protein O-GlcNAcase
Identifiers
EC number	3.2.1.169
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Protein O-GlcNAcase (EC 3.2.1.169, OGA, glycoside hydrolase O-GlcNAcase, O-GlcNAcase, BtGH84, O-GlcNAc hydrolase) is an enzyme with systematic name (protein)-3-O-(N-acetyl-D-glucosaminyl)-L-serine/threonine N-acetylglucosaminyl hydrolase.^{[1][2][3][4][5][6]} This enzyme catalyses the following chemical reaction

(1) [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H₂O $\rightleftharpoons$ [protein]-L-serine + N-acetyl-D-glucosamine

(2) [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H₂O $\rightleftharpoons$ [protein]-L-threonine + N-acetyl-D-glucosamine

References

1. Gao, Y., Wells, L., Comer, F.I., Parker, G.J. and Hart, G.W. (2001). "Dynamic O-glycosylation of nuclear and cytosolic proteins: cloning and characterization of a neutral, cytosolic β-N-acetylglucosaminidase from human brain". J. Biol. Chem. 276: 9838–9845. doi:10.1074/jbc.M010420200. PMID 11148210.
2. Wells, L., Gao, Y., Mahoney, J.A., Vosseller, K., Chen, C., Rosen, A. and Hart, G.W. (2002). "Dynamic O-glycosylation of nuclear and cytosolic proteins: further characterization of the nucleocytoplasmic β-N-acetylglucosaminidase, O-GlcNAcase". J. Biol. Chem. 277 (3): 1755–1761. doi:10.1074/jbc.M109656200. PMID 11788610.
3. Cetinbas, N., Macauley, M.S., Stubbs, K.A., Drapala, R. and Vocadlo, D.J. (2006). "Identification of Asp¹⁷⁴ and Asp¹⁷⁵ as the key catalytic residues of human O-GlcNAcase by functional analysis of site-directed mutants". Biochemistry 45 (11): 3835–3844. doi:10.1021/bi052370b. PMID 16533067.
4. Dennis, R.J., Taylor, E.J., Macauley, M.S., Stubbs, K.A., Turkenburg, J.P., Hart, S.J., Black, G.N., Vocadlo, D.J. and Davies, G.J. (2006). "Structure and mechanism of a bacterial β-glucosaminidase having O-GlcNAcase activity". Nat. Struct. Mol. Biol. 13 (4): 365–371. doi:10.1038/nsmb1079. PMID 16565725.
5. Kim, E.J., Kang, D.O., Love, D.C. and Hanover, J.A. (2006). "Enzymatic characterization of O-GlcNAcase isoforms using a fluorogenic GlcNAc substrate". Carbohydr. Res. 341 (8): 971–982. doi:10.1016/j.carres.2006.03.004. PMID 16584714.
6. Dong, D.L. and Hart, G.W. (1994). "Purification and characterization of an O-GlcNAc selective N-acetyl-β-D-glucosaminidase from rat spleen cytosol". J. Biol. Chem. 269 (30): 19321–19330. PMID 8034696.

External links

• Protein O-GlcNAcase at the US National Library of Medicine Medical Subject Headings (MeSH)