Rhamnogalacturonan hydrolase

Rhamnogalacturonan hydrolase (EC 3.2.1.171, rhamnogalacturonase A, RGase A, RG-hydrolase) is an enzyme with systematic name rhamnogalacturonan alpha-D-GalA-(1->2)-alpha-L-Rha hydrolase.^[1]^[2]^[3]^[4]^[5] This enzyme catalyses the following chemical reaction

Endohydrolysis of alpha-D-GalA-(1->2)-alpha-L-Rha glycosidic bond in the rhamnogalacturonan I backbone with initial inversion of anomeric configuration releasing oligosaccharides with beta-D-GalA at the reducing end.

The enzyme is part of the degradation system for rhamnogalacturonan I in Aspergillus aculeatus.

References

↑ Petersen, T.N., Kauppinen, S. and Larsen, S. (1997). "The crystal structure of rhamnogalacturonase A from Aspergillus aculeatus: a right-handed parallel β helix". Structure 5: 533–544. doi:10.1016/s0969-2126(97)00209-8. PMID 9115442.
↑ Kofod, L.V., Kauppinen, S., Christgau, S., Andersen, L.N., Heldt-Hansen, H.P., Dorreich, K. and Dalboge, H. (1994). "Cloning and characterization of two structurally and functionally divergent rhamnogalacturonases from Aspergillus aculeatus". J. Biol. Chem. 269: 29182–29189. PMID 7961884.
↑ Azadi, P., O'Neill, M.A., Bergmann, C., Darvill, A.G. and Albersheim, P. (1995). "The backbone of the pectic polysaccharide rhamnogalacturonan I is cleaved by an endohydrolase and an endolyase". Glycobiology 5: 783–789. doi:10.1093/glycob/5.8.783. PMID 8720076.
↑ Petersen, T.N., Christgau, S., Kofod, L.V., Kauppinen, S., Johnson, A.H. and Larsen, S. (1997). "Crystallization and preliminary X-ray studies of rhamnogalacturonase A from Aspergillus aculeatus". Acta Crystallogr. D Biol. Crystallogr. 53: 105–107. doi:10.1107/s0907444996010785. PMID 15299976.
↑ Pitson, S.M., Mutter, M., van den Broek, L.A., Voragen, A.G. and Beldman, G. (1998). "Stereochemical course of hydrolysis catalysed by α-L-rhamnosyl and α-D-galacturonosyl hydrolases from Aspergillus aculeatus". Biochem. Biophys. Res. Commun. 242: 552–559. doi:10.1006/bbrc.1997.8009. PMID 9464254.

External links

Rhamnogalacturonan hydrolase at the US National Library of Medicine Medical Subject Headings (MeSH)

Hydrolase: sugar hydrolases (EC 3.2)

3.2.1: Glycoside hydrolases

Disaccharidase	Sucrase/Sucrase-isomaltase/Invertase Maltase Trehalase Lactase

Glucosidases	Cellulase Alpha-glucosidase Acid Neutral AB Neutral C Beta-glucosidase cytosolic Debranching enzyme

Other	Amylase Alpha-amylase Chitinase Lysozyme Neuraminidase NEU1 NEU2 NEU3 NEU4 Bacterial neuraminidase Viral neuraminidase Galactosidases Alpha Beta alpha-Mannosidase Glucuronidase Hyaluronidase Pullulanase Glucosylceramidase lysosomal non-lysosomal Galactosylceramidase Alpha-N-acetylgalactosaminidase NAGA Alpha-N-acetylglucosaminidase Fucosidase Hexosaminidase HEXA HEXB Iduronidase Maltase-glucoamylase Heparanase HPSE2

3.2.2: Hydrolysing
N-Glycosyl compounds

DNA glycosylases: Oxoguanine glycosylase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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