Centromere protein E

Centromere protein E, 312kDa
PDB rendering based on 1t5c.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1T5C​
Identifiers
Symbols	CENPE ; CENP-E; KIF10; MCPH13; PPP1R61
External IDs	OMIM: 117143 MGI: 1098230 HomoloGene: 20429 ChEMBL: 5870 GeneCards: CENPE Gene
Gene ontology Molecular function • microtubule motor activity • protein binding • ATP binding • microtubule binding • ATPase activity • kinetochore binding Cellular component • chromosome, centromeric region • kinetochore • condensed chromosome, centromeric region • condensed chromosome outer kinetochore • nucleus • chromosome • cytoplasm • cytosol • kinesin complex • microtubule • microtubule cytoskeleton • membrane • midbody • mitotic spindle midzone Biological process • mitotic cell cycle • microtubule-based movement • chromosome segregation • mitotic chromosome movement towards spindle pole • mitotic metaphase plate congression • small GTPase mediated signal transduction • multicellular organismal development • blood coagulation • metabolic process • antigen processing and presentation of exogenous peptide antigen via MHC class II • regulation of mitotic metaphase/anaphase transition • positive regulation of protein kinase activity • cell division • attachment of mitotic spindle microtubules to kinetochore • kinetochore assembly Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	1062	229841
Ensembl	ENSG00000138778	ENSMUSG00000045328
UniProt	Q02224	Q6RT24
RefSeq (mRNA)	NM_001286734	NM_173762
RefSeq (protein)	NP_001273663	NP_776123
Location (UCSC)	Chr 4: 103.11 – 103.2 Mb	Chr 3: 135.21 – 135.27 Mb
PubMed search

Centromere-associated protein E is a protein that in humans is encoded by the CENPE gene.^[1][2]

Centromere-associated protein E is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centromere-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. CENPE is proposed to be one of the motors responsible for mammalian chromosome movement and/or spindle elongation.^[2]

References

1. Testa JR, Zhou JY, Bell DW, Yen TJ (Mar 1995). "Chromosomal localization of the genes encoding the kinetochore proteins CENPE and CENPF to human chromosomes 4q24→q25 and 1q32→q41, respectively, by fluorescence in situ hybridization". Genomics 23 (3): 691–3. doi:10.1006/geno.1994.1558. PMID 7851898.
2. "Entrez Gene: CENPE centromere protein E, 312kDa".

Further reading

• Yen TJ, Li G, Schaar BT, et al. (1992). "CENP-E is a putative kinetochore motor that accumulates just before mitosis". Nature 359 (6395): 536–9. doi:10.1038/359536a0. PMID 1406971.
• Yen TJ, Compton DA, Wise D, et al. (1991). "CENP-E, a novel human centromere-associated protein required for progression from metaphase to anaphase". EMBO J. 10 (5): 1245–54. PMC 452779. PMID 2022189.
• Thrower DA, Jordan MA, Schaar BT, et al. (1995). "Mitotic HeLa cells contain a CENP-E-associated minus end-directed microtubule motor". EMBO J. 14 (5): 918–26. PMC 398164. PMID 7889940.
• Wood KW, Sakowicz R, Goldstein LS, Cleveland DW (1997). "CENP-E is a plus end-directed kinetochore motor required for metaphase chromosome alignment". Cell 91 (3): 357–66. doi:10.1016/S0092-8674(00)80419-5. PMID 9363944.
• Cooke CA, Schaar B, Yen TJ, Earnshaw WC (1998). "Localization of CENP-E in the fibrous corona and outer plate of mammalian kinetochores from prometaphase through anaphase". Chromosoma 106 (7): 446–55. doi:10.1007/s004120050266. PMID 9391217.
• Zecevic M, Catling AD, Eblen ST, et al. (1998). "Active MAP kinase in mitosis: localization at kinetochores and association with the motor protein CENP-E". J. Cell Biol. 142 (6): 1547–58. doi:10.1083/jcb.142.6.1547. PMC 2141767. PMID 9744883.
• Chan GK, Schaar BT, Yen TJ (1998). "Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1". J. Cell Biol. 143 (1): 49–63. doi:10.1083/jcb.143.1.49. PMC 2132809. PMID 9763420.
• Chan GK, Jablonski SA, Sudakin V, et al. (1999). "Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC". J. Cell Biol. 146 (5): 941–54. doi:10.1083/jcb.146.5.941. PMC 2169490. PMID 10477750.
• Ashar HR, James L, Gray K, et al. (2000). "Farnesyl transferase inhibitors block the farnesylation of CENP-E and CENP-F and alter the association of CENP-E with the microtubules". J. Biol. Chem. 275 (39): 30451–7. doi:10.1074/jbc.M003469200. PMID 10852915.
• Uren AG, Wong L, Pakusch M, et al. (2001). "Survivin and the inner centromere protein INCENP show similar cell-cycle localization and gene knockout phenotype". Curr. Biol. 10 (21): 1319–28. doi:10.1016/S0960-9822(00)00769-7. PMID 11084331.
• Suzuki Y, Tsunoda T, Sese J, et al. (2001). "Identification and characterization of the potential promoter regions of 1031 kinds of human genes". Genome Res. 11 (5): 677–84. doi:10.1101/gr.164001. PMC 311086. PMID 11337467.
• Liu ST, Chan GK, Hittle JC, et al. (2003). "Human MPS1 kinase is required for mitotic arrest induced by the loss of CENP-E from kinetochores". Mol. Biol. Cell 14 (4): 1638–51. doi:10.1091/mbc.02-05-0074. PMC 153128. PMID 12686615.
• Brandenberger R, Wei H, Zhang S, et al. (2005). "Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation". Nat. Biotechnol. 22 (6): 707–16. doi:10.1038/nbt971. PMID 15146197.
• Garcia-Saez I, Blot D, Kahn R, Kozielski F (2005). "Crystallization and preliminary crystallographic analysis of the motor domain of human kinetochore-associated protein CENP-E using an automated crystallization procedure". Acta Crystallogr. D Biol. Crystallogr. 60 (Pt 6): 1158–60. doi:10.1107/S0907444904009564. PMID 15159587.
• Tanudji M, Shoemaker J, L'Italien L, et al. (2005). "Gene silencing of CENP-E by small interfering RNA in HeLa cells leads to missegregation of chromosomes after a mitotic delay". Mol. Biol. Cell 15 (8): 3771–81. doi:10.1091/mbc.E03-07-0482. PMC 491836. PMID 15181147.
• Garcia-Saez I, Yen T, Wade RH, Kozielski F (2004). "Crystal structure of the motor domain of the human kinetochore protein CENP-E". J. Mol. Biol. 340 (5): 1107–16. doi:10.1016/j.jmb.2004.05.053. PMID 15236970.
• Kurasawa Y, Earnshaw WC, Mochizuki Y, et al. (2005). "Essential roles of KIF4 and its binding partner PRC1 in organized central spindle midzone formation". EMBO J. 23 (16): 3237–48. doi:10.1038/sj.emboj.7600347. PMC 514520. PMID 15297875.
• Mao Y, Desai A, Cleveland DW (2005). "Microtubule capture by CENP-E silences BubR1-dependent mitotic checkpoint signaling". J. Cell Biol. 170 (6): 873–80. doi:10.1083/jcb.200505040. PMC 2171436. PMID 16144904.
• Nousiainen M, Silljé HH, Sauer G, et al. (2006). "Phosphoproteome analysis of the human mitotic spindle". Proc. Natl. Acad. Sci. U.S.A. 103 (14): 5391–6. doi:10.1073/pnas.0507066103. PMC 1459365. PMID 16565220.