Glycoside hydrolase family 25

Glycosyl hydrolases family 25
crystal structure of the bacterial lysozyme from streptomyces coelicolor at 1.65 a resolution
Identifiers
Symbol	Glyco_hydro_25
Pfam	PF01183
Pfam clan	CL0058
InterPro	IPR002053
PROSITE	PDOC00737
SCOP	1jfx
SUPERFAMILY	1jfx
CAZy	GH25
Available protein structures: Pfam structures PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

In molecular biology, glycoside hydrolase family 25 is a family of glycoside hydrolases.

Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families.^[1][2][3] This classification is available on the CAZy(http://www.cazy.org/GH1.html) web site,^[4] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.^[5]

Glycoside hydrolase family 25 CAZY GH_25 comprises enzymes with only one known activity; lysozyme (EC 3.2.1.17). It has been shown^[6][7] that a number of cell-wall lytic enzymes are evolutionary related and can be classified into a single family. Two residues, an aspartate and a glutamate, have been shown^[8] to be important for the catalytic activity of the Charalopsis enzyme. These residues as well as some others in their vicinity are conserved in all proteins from this family.

References

1. Henrissat B, Callebaut I, Mornon JP, Fabrega S, Lehn P, Davies G (1995). "Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases". Proc. Natl. Acad. Sci. U.S.A. 92 (15): 7090–7094. doi:10.1073/pnas.92.15.7090. PMC 41477. PMID 7624375.
2. Henrissat B, Davies G (1995). "Structures and mechanisms of glycosyl hydrolases". Structure 3 (9): 853–859. doi:10.1016/S0969-2126(01)00220-9. PMID 8535779.
3. Bairoch, A. "Classification of glycosyl hydrolase families and index of glycosyl hydrolase entries in SWISS-PROT". 1999.
4. Henrissat, B. and Coutinho P.M. "Carbohydrate-Active Enzymes server". 1999.
5. CAZypedia, an online encyclopedia of carbohydrate-active enzymes.
6. Henrissat B (December 1991). "A classification of glycosyl hydrolases based on amino acid sequence similarities". Biochem. J. 280 (2): 309–16. PMC 1130547. PMID 1747104.
7. Croux C, Garcia JL (1991). "Sequence of the lyc gene encoding the autolytic lysozyme of Clostridium acetobutylicum ATCC824: comparison with other lytic enzymes". Gene 104 (1): 25–31. doi:10.1016/0378-1119(91)90460-S. PMID 1916274.
8. Fouche PB, Hash JH (1978). "The N,O-diacetylmuramidase of Chalaropsis species. Identification of aspartyl and glutamyl residues in the active site". J. Biol. Chem. 253 (19): 6787–6793. PMID 567645.

This article incorporates text from the public domain Pfam and InterPro IPR002053