Glycoside hydrolase family 32

Glycosyl hydrolases family 32 N-terminal domain
beta-fructosidase from thermotoga maritima in complex with raffinose
Identifiers
Symbol	Glyco_hydro_32N
Pfam	PF00251
Pfam clan	CL0143
InterPro	IPR013148
PROSITE	PDOC00532
CAZy	GH32
Available protein structures: Pfam structures PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

Glycosyl hydrolases family 32 C terminal
crystal structure of exo-inulinase from aspergillus awamori in spacegroup p21
Identifiers
Symbol	Glyco_hydro_32C
Pfam	PF08244
InterPro	IPR013189
PROSITE	PDOC00532
CAZy	GH32
Available protein structures: Pfam structures PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

In molecular biology, glycoside hydrolase family 32 is a family of glycoside hydrolases.

Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families.^[1][2][3] This classification is available on the CAZy(http://www.cazy.org/GH1.html) web site,^[4] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.^[5]

Family 32 glycosyl hydrolases comprise two distinct domains. The N-terminal domain, which forms a five bladed beta propeller, and the C-terminal domain, which forms a beta sandwich structure.^[6]

References

1. Henrissat B, Callebaut I, Mornon JP, Fabrega S, Lehn P, Davies G (1995). "Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases". Proc. Natl. Acad. Sci. U.S.A. 92 (15): 7090–7094. doi:10.1073/pnas.92.15.7090. PMC 41477. PMID 7624375.
2. Henrissat B, Davies G (1995). "Structures and mechanisms of glycosyl hydrolases". Structure 3 (9): 853–859. doi:10.1016/S0969-2126(01)00220-9. PMID 8535779.
3. Bairoch, A. "Classification of glycosyl hydrolase families and index of glycosyl hydrolase entries in SWISS-PROT". 1999.
4. Henrissat, B. and Coutinho P.M. "Carbohydrate-Active Enzymes server". 1999.
5. CAZypedia, an online encyclopedia of carbohydrate-active enzymes.
6. Alberto F, Bignon C, Sulzenbacher G, Henrissat B, Czjzek M (2004). "The three-dimensional structure of invertase (beta-fructosidase) from Thermotoga maritima reveals a bimodular arrangement and an evolutionary relationship between retaining and inverting glycosidases.". J Biol Chem 279 (18): 18903–10. doi:10.1074/jbc.M313911200. PMID 14973124.

This article incorporates text from the public domain Pfam and InterPro IPR013189

This article incorporates text from the public domain Pfam and InterPro IPR013148