Glycoside hydrolase family 4

Family 4 glycosyl hydrolase
structure of the 6-phospho-beta glucosidase from thermotoga maritima at 2.55 angstrom resolution in the tetragonal form with manganese, nad+ and glucose-6-phosphate
Identifiers
Symbol	Glyco_hydro_4
Pfam	PF02056
Pfam clan	CL0063
InterPro	IPR001088
PROSITE	PDOC01027
SCOP	1obb
SUPERFAMILY	1obb
CAZy	GH4
Available protein structures: Pfam structures PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

Family 4 glycosyl hydrolase C-terminal domain
Identifiers
Symbol	Glyco_hydro_4C
Pfam	PF11975
Pfam clan	CL0341
PROSITE	PDOC01027
SCOP	1obb
SUPERFAMILY	1obb
CAZy	GH4
Available protein structures: Pfam structures PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

In molecular biology, glycoside hydrolase family 4 is a family of glycoside hydrolases.

Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families.^[1][2][3] This classification is available on the CAZy(http://www.cazy.org/GH1.html) web site,^[4] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.^[5]

Glycoside hydrolase family 4 CAZY GH_4 comprises enzymes with several known activities; 6-phospho-beta-glucosidase (EC 3.2.1.86); 6-phospho-alpha-glucosidase (EC 3.2.1.122); alpha-galactosidase (EC 3.2.1.22). 6-phospho-alpha-glucosidase requires both NAD(H) and divalent metal (Mn2+, Fe2+, Co2+, or Ni2+) for activity.^[6]

External references

GH4 in CAZypedia

References

1. Henrissat B, Callebaut I, Mornon JP, Fabrega S, Lehn P, Davies G (1995). "Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases". Proc. Natl. Acad. Sci. U.S.A. 92 (15): 7090–7094. doi:10.1073/pnas.92.15.7090. PMC 41477. PMID 7624375.
2. Henrissat B, Davies G (1995). "Structures and mechanisms of glycosyl hydrolases". Structure 3 (9): 853–859. doi:10.1016/S0969-2126(01)00220-9. PMID 8535779.
3. Bairoch, A. "Classification of glycosyl hydrolase families and index of glycosyl hydrolase entries in SWISS-PROT". 1999.
4. Henrissat, B. and Coutinho P.M. "Carbohydrate-Active Enzymes server". 1999.
5. CAZypedia, an online encyclopedia of carbohydrate-active enzymes.
6. Henrissat B, Thompson J, Yamamoto H, Pikis A, Ruvinov SB, Sekiguchi J (1998). "The gene glvA of Bacillus subtilis 168 encodes a metal-requiring, NAD(H)-dependent 6-phospho-alpha-glucosidase. Assignment to family 4 of the glycosylhydrolase superfamily". J. Biol. Chem. 273 (42): 27347–27356. doi:10.1074/jbc.273.42.27347. PMID 9765262.

This article incorporates text from the public domain Pfam and InterPro IPR001088