Glycoside hydrolase family 45

Glycosyl hydrolase family 45

endoglucanase from humicola insolens at 1.7a resolution
Identifiers
Symbol Glyco_hydro_45
Pfam PF02015
Pfam clan CL0199
InterPro IPR000334
PROSITE PDOC00877
SCOP 2eng
SUPERFAMILY 2eng
CAZy GH45

In molecular biology, glycoside hydrolase family 45 is a family of glycoside hydrolases.

Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families.[1][2][3] This classification is available on the CAZy(http://www.cazy.org/GH1.html) web site,[4] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.[5]

Glycoside hydrolase family 45 CAZY GH_45 comprises enzymes with only one known activity; endoglucanase (EC 3.2.1.4). This family is also known as cellulase family K.[6] The best conserved region in these enzymes is located in the N-terminal section. It contains an aspartic acid residue which has been shown[7] to act as a nucleophile in the catalytic mechanism. This also has several cysteines that are involved in forming disulphide bridges.

References

  1. Henrissat B, Callebaut I, Mornon JP, Fabrega S, Lehn P, Davies G (1995). "Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases". Proc. Natl. Acad. Sci. U.S.A. 92 (15): 7090–7094. doi:10.1073/pnas.92.15.7090. PMC 41477. PMID 7624375.
  2. Henrissat B, Davies G (1995). "Structures and mechanisms of glycosyl hydrolases". Structure 3 (9): 853–859. doi:10.1016/S0969-2126(01)00220-9. PMID 8535779.
  3. Bairoch, A. "Classification of glycosyl hydrolase families and index of glycosyl hydrolase entries in SWISS-PROT". 1999.
  4. Henrissat, B. and Coutinho P.M. "Carbohydrate-Active Enzymes server". 1999.
  5. CAZypedia, an online encyclopedia of carbohydrate-active enzymes.
  6. Henrissat B, Bairoch A (1993). "New families in the classification of glycosyl hydrolases based on amino acid sequence similarities". Biochem. J. 293: 781–788. PMC 1134435. PMID 8352747.
  7. Dodson GG, Schulein M, Davies GJ, Dauter Z, Wilson KS, Hubbard RE, Tolley SP, Hjort C, Mikkelsen JM, Rasmussen G (1993). "Structure and function of endoglucanase V". Nature 365 (6444): 362–364. doi:10.1038/365362a0. PMID 8377830.

This article incorporates text from the public domain Pfam and InterPro IPR000334

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